Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas
Abstract
:1. Introduction
2. Materials and Methods
2.1. HCMV Neutralizing Monoclonal and Control Antibodies
2.2. Primary Cells and Human Placentas
2.3. Virus Neutralization Assays
2.4. Virus Spread Inhibition Assays
2.5. HCMV Titration
2.6. Antibodies and Reagents
2.7. Immunofluorescence and Imaging
3. Results
3.1. mAbs Have Potent HCMV Neutralizing Activities in Primary Placental Cells
3.1.1. Human Placental Fibroblast (HPF) Infection Is Blocked by mAbs to gB and gH/gL
3.1.2. Trophoblast Progenitor Cell (TBPC) Infection Is Blocked by mAbs to gB and gH/gL
3.1.3. Amniotic Epithelial Cell (AmEpC) Infection Is Strongly Inhibited by Anti-Pentamer mAbs
3.2. mAbs Specific to HCMV Proteins Neutralize Infection of Cell Column CTBs in Anchoring Villus Explants
3.3. Hofbauer Cells in Villus Cores Phagocytose IgG-Virion Complexes
3.4. Antibody Inhibition of Cell–Cell Spread of HCMV and Apoptosis in CTB Cell Columns in Anchoring Villus Explants
3.5. Neutralizing Antibodies Inhibit Cell–Cell Spread of HCMV in ARPE-19 Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Tabata, T.; Petitt, M.; Fang-Hoover, J.; Freed, D.C.; Li, F.; An, Z.; Wang, D.; Fu, T.-M.; Pereira, L. Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas. Vaccines 2019, 7, 135. https://doi.org/10.3390/vaccines7040135
Tabata T, Petitt M, Fang-Hoover J, Freed DC, Li F, An Z, Wang D, Fu T-M, Pereira L. Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas. Vaccines. 2019; 7(4):135. https://doi.org/10.3390/vaccines7040135
Chicago/Turabian StyleTabata, Takako, Matthew Petitt, June Fang-Hoover, Daniel C. Freed, Fengsheng Li, Zhiqiang An, Dai Wang, Tong-Ming Fu, and Lenore Pereira. 2019. "Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas" Vaccines 7, no. 4: 135. https://doi.org/10.3390/vaccines7040135
APA StyleTabata, T., Petitt, M., Fang-Hoover, J., Freed, D. C., Li, F., An, Z., Wang, D., Fu, T. -M., & Pereira, L. (2019). Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas. Vaccines, 7(4), 135. https://doi.org/10.3390/vaccines7040135