Adjuvantation of Pulmonary-Administered Influenza Vaccine with GPI-0100 Primarily Stimulates Antibody Production and Memory B Cell Proliferation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Virus and Vaccine Preparation
2.2. Immunization, Challenge and Sample Collection
2.3. ELISA
2.4. Virus Titration
2.5. ELISpot
2.6. Flow Cytometry
2.7. Statistical Analysis
3. Results
3.1. Systemic Antibody Responses
3.2. Mucosal Antibody Responses
3.3. Analysis of B Cell Responses
3.4. Analysis of Cytokine Responses
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Appendix A
References
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Patil, H.P.; Herrera Rodriguez, J.; De Vries-Idema, J.; Meijerhof, T.; Frijlink, H.W.; Hinrichs, W.L.J.; Huckriede, A. Adjuvantation of Pulmonary-Administered Influenza Vaccine with GPI-0100 Primarily Stimulates Antibody Production and Memory B Cell Proliferation. Vaccines 2017, 5, 19. https://doi.org/10.3390/vaccines5030019
Patil HP, Herrera Rodriguez J, De Vries-Idema J, Meijerhof T, Frijlink HW, Hinrichs WLJ, Huckriede A. Adjuvantation of Pulmonary-Administered Influenza Vaccine with GPI-0100 Primarily Stimulates Antibody Production and Memory B Cell Proliferation. Vaccines. 2017; 5(3):19. https://doi.org/10.3390/vaccines5030019
Chicago/Turabian StylePatil, Harshad P., José Herrera Rodriguez, Jacqueline De Vries-Idema, Tjarko Meijerhof, Henderik W. Frijlink, Wouter L. J. Hinrichs, and Anke Huckriede. 2017. "Adjuvantation of Pulmonary-Administered Influenza Vaccine with GPI-0100 Primarily Stimulates Antibody Production and Memory B Cell Proliferation" Vaccines 5, no. 3: 19. https://doi.org/10.3390/vaccines5030019
APA StylePatil, H. P., Herrera Rodriguez, J., De Vries-Idema, J., Meijerhof, T., Frijlink, H. W., Hinrichs, W. L. J., & Huckriede, A. (2017). Adjuvantation of Pulmonary-Administered Influenza Vaccine with GPI-0100 Primarily Stimulates Antibody Production and Memory B Cell Proliferation. Vaccines, 5(3), 19. https://doi.org/10.3390/vaccines5030019