Unbiased Identification of Dengue Virus Non-Structural Protein 1 Peptides for Use in Vaccine Design
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of NS1 Peptides
2.2. Qβ VLP Vaccine Production
2.3. Enzyme-Linked Immunosorbent Assays (ELISAs)
2.4. Animals
2.5. Cells and Viral Stocks
2.6. Anti-NS1 Antiserum Binding to DENV-Infected HEK-293 Cells
3. Results
3.1. Strategy for Unbiased Identification of NS1 Peptide Eptiopes of Interest
3.2. Immunogenicity of Qβ VLPs Displaying Unbiased NS1 Peptide Epitopes
3.3. Structural Locations of Down-Selected NS1 Peptide Epitopes
3.4. Binding Characteristics of IgG Elicited by Qβ VLPs Displaying NS1 Peptides Epitopes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Warner, N.L.; Core, S.B.; Frietze, K.M. Unbiased Identification of Dengue Virus Non-Structural Protein 1 Peptides for Use in Vaccine Design. Vaccines 2022, 10, 2028. https://doi.org/10.3390/vaccines10122028
Warner NL, Core SB, Frietze KM. Unbiased Identification of Dengue Virus Non-Structural Protein 1 Peptides for Use in Vaccine Design. Vaccines. 2022; 10(12):2028. https://doi.org/10.3390/vaccines10122028
Chicago/Turabian StyleWarner, Nikole L., Susan B. Core, and Kathryn M. Frietze. 2022. "Unbiased Identification of Dengue Virus Non-Structural Protein 1 Peptides for Use in Vaccine Design" Vaccines 10, no. 12: 2028. https://doi.org/10.3390/vaccines10122028
APA StyleWarner, N. L., Core, S. B., & Frietze, K. M. (2022). Unbiased Identification of Dengue Virus Non-Structural Protein 1 Peptides for Use in Vaccine Design. Vaccines, 10(12), 2028. https://doi.org/10.3390/vaccines10122028