Next Article in Journal
Long-Term Antibody Response against SARS-CoV-2 in Health Care Workers: Effectiveness of Homologous and Heterologous Regimens and Their Relation to Systemic Vaccine-Associated Symptoms
Previous Article in Journal
Identification of Shared Neoantigens in BRCA1-Related Breast Cancer
Previous Article in Special Issue
Multi-Epitope-Based Vaccine Candidate for Monkeypox: An In Silico Approach
 
 

This is an early access version, the complete PDF, HTML, and XML versions will be available soon.

Article

Immunoinformatics Studies and Design of a Potential Multi-Epitope Peptide Vaccine to Combat the Fatal Visceral Leishmaniasis

1
Biotechnology Programme, Department of Biological Sciences, Elizade University, P.M.B, 002 Ilara-Mokin, Ilara-Mokin 340271, Nigeria
2
Division of Immunology, Faculty of Health Sciences, Institute of Infectious Diseases and Molecular Medicine (IDM), University of Cape Town, Cape Town 7925, South Africa
3
Department of Chemistry, Bayelsa Medical University, Yenagoa, Yenagoa 560001, Nigeria
4
Department of Biochemistry, School of Life Sciences (SLS), Federal University of Technology Akure, Akure 340110, Nigeria
5
Microbiology Programme, Department of Biological Sciences, Elizade University, Ilara-Mokin 340271, Nigeria
6
Department of Molecular and Cell Biology, Faculty of Science, University of Cape Town, Cape Town 7701, South Africa
*
Authors to whom correspondence should be addressed.
Vaccines 2022, 10(10), 1598; https://doi.org/10.3390/vaccines10101598
Received: 9 August 2022 / Revised: 12 September 2022 / Accepted: 13 September 2022 / Published: 22 September 2022
(This article belongs to the Collection Vaccines against Infectious Diseases)
Leishmaniasis is a neglected tropical disease caused by parasitic intracellular protozoa of the genus Leishmania. The visceral form of this disease caused by Leishmania donovani continues to constitute a major public health crisis, especially in countries of endemicity. In some cases, it is asymptomatic and comes with acute and chronic clinical outcomes such as weight loss, pancytopenia, hepatosplenomegaly, and death if left untreated. Over the years, the treatment of VL has relied solely on chemotherapeutic agents, but unfortunately, these drugs are now faced with challenges. Despite all efforts, no successful vaccine has been approved for VL. This could be as a result of limited knowledge/understanding of the immune mechanisms necessary to regulate parasite growth. Using a computational approach, this study explored the prospect of harnessing the properties of a disulfide isomerase protein of L. donovani amastigotses to develop a multi-epitope subunit vaccine candidate against the parasite. We designed a 248-amino acid multi-epitope vaccine with a predicted antigenicity probability of 0.897372. Analyses of immunogenicity, allergenicity, and multiple physiochemical parameters indicated that the constructed vaccine candidate was stable, non-allergenic, and immunogenic, making it compatible with humans and hence, a potentially viable and safe vaccine candidate against Leishmania spp. Parasites.
Keywords: visceral leishmaniasis; Leishmania donovani; vaccine; epitopes; cytotoxic T-cells; helper T-cells; TLRs visceral leishmaniasis; Leishmania donovani; vaccine; epitopes; cytotoxic T-cells; helper T-cells; TLRs
MDPI and ACS Style

Onile, O.S.; Musaigwa, F.; Ayawei, N.; Omoboyede, V.; Onile, T.A.; Oghenevovwero, E.; Aruleba, R.T. Immunoinformatics Studies and Design of a Potential Multi-Epitope Peptide Vaccine to Combat the Fatal Visceral Leishmaniasis. Vaccines 2022, 10, 1598. https://doi.org/10.3390/vaccines10101598

AMA Style

Onile OS, Musaigwa F, Ayawei N, Omoboyede V, Onile TA, Oghenevovwero E, Aruleba RT. Immunoinformatics Studies and Design of a Potential Multi-Epitope Peptide Vaccine to Combat the Fatal Visceral Leishmaniasis. Vaccines. 2022; 10(10):1598. https://doi.org/10.3390/vaccines10101598

Chicago/Turabian Style

Onile, Olugbenga Samson, Fungai Musaigwa, Nimibofa Ayawei, Victor Omoboyede, Tolulope Adelonpe Onile, Eyarefe Oghenevovwero, and Raphael Taiwo Aruleba. 2022. "Immunoinformatics Studies and Design of a Potential Multi-Epitope Peptide Vaccine to Combat the Fatal Visceral Leishmaniasis" Vaccines 10, no. 10: 1598. https://doi.org/10.3390/vaccines10101598

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop