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Open AccessArticle

Andrographis paniculata and Its Bioactive Diterpenoids Protect Dermal Fibroblasts against Inflammation and Oxidative Stress

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Laboratory I3MTO, EA 4708, Université d’Orléans, CEDEX 2, 45067 Orléans, France
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NOVAXIA-6 Rue des Champs Godin, 41220 St Laurent Nouan, France
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Service de Rhumatologie, Centre Hospitalier Régional d’Orléans CHRO, 14 Avenue de l’Hôpital, 45100 Orléans, France
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Plateforme Recherche Innovation Médicale Mutualisée d’Orléans, Centre Hospitalier Régional d’Orléans 14 Avenue de l’Hôpital, 45100 Orléans, France
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Service de Dermatologie, Centre Hospitalier Régional d′Orléans 14 Avenue de l’Hôpital, 45100 Orléans, France
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Institut de Chimie Organique et Analytique ICOA, Université d’Orléans-Pôle de Chimie, UMR CNRS 7311, Rue de Chartres-BP 6759, CEDEX 2, 45067 Orléans, France
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Author to whom correspondence should be addressed.
Antioxidants 2020, 9(5), 432; https://doi.org/10.3390/antiox9050432
Received: 22 April 2020 / Revised: 8 May 2020 / Accepted: 13 May 2020 / Published: 15 May 2020
Andrographis paniculata (Burm.f.) has long been used in ayurvedic medicine through its anti-inflammatory properties. However, its protective effect of skin aging has not been studied in vitro. This study aimed to investigate the anti-aging effects of methanolic extract (ME), andrographolide (ANDRO), neoandrographolide (NEO), 14-deoxyandrographolide (14DAP) and 14-deoxy-11,12-didehydroandrographolide (14DAP11-12) on human dermal fibroblasts (HDFa) under pro-oxidant or pro-inflammatory condition. The in vitro anti-aging capacity of ME, ANDRO, NEO, 14DAP, and 14DAP11-12 (1, 2.5 and 5 µg/mL) was performed in HDFa. Oxidative stress and inflammation were induced by hydrogen peroxide and lipopolysaccharide/TNF-α, respectively. Reactive oxygen species (ROS) production was measured by the fluorescence of DCF-DA probe and cytokines were quantified by ELISA (IL6 and IL8) or RTqPCR (TNF-α). Procollagen type I production was determined by an ELISA. Our results showed a decrease in ROS production with ME and 14DAP at 5 µg/mL and 1 µg/mL, respectively. Furthermore, IL-6 production and TNF-α expression decreased under ANDRO and ME at 5 µg/mL. Our data indicated that ME and 14DAP protect from oxidative stress. Additionally, ME and ANDRO decreased an inflammation marker, IL-6. This suggests their potential natural treatment against skin damage. Hence, their applications could be of interest in cosmetics for preventing skin ageing. View Full-Text
Keywords: Andrographis paniculata; dermal fibroblast; inflammation; oxidative stress; skin aging; biomolecules; natural products Andrographis paniculata; dermal fibroblast; inflammation; oxidative stress; skin aging; biomolecules; natural products
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MDPI and ACS Style

Mussard, E.; Jousselin, S.; Cesaro, A.; Legrain, B.; Lespessailles, E.; Esteve, E.; Berteina-Raboin, S.; Toumi, H. Andrographis paniculata and Its Bioactive Diterpenoids Protect Dermal Fibroblasts against Inflammation and Oxidative Stress. Antioxidants 2020, 9, 432.

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