Next Article in Journal
Maternal Antioxidant Status in Early Pregnancy and Development of Fetal Complications in Twin Pregnancies: A Pilot Study
Next Article in Special Issue
Natural Phenolic Compounds for Health, Food and Cosmetic Applications
Previous Article in Journal
In Vitro and In Vivo Nutraceutical Characterization of Two Chickpea Accessions: Differential Effects on Hepatic Lipid Over-Accumulation
Previous Article in Special Issue
Gelatin-Based Hydrogels for the Controlled Release of 5,6-Dihydroxyindole-2-Carboxylic Acid, a Melanin-Related Metabolite with Potent Antioxidant Activity
Open AccessArticle

A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications

Department of Chemical Sciences, University of Naples “Federico II”, Via Cintia 4, I-80126 Naples, Italy
*
Authors to whom correspondence should be addressed.
Antioxidants 2020, 9(4), 270; https://doi.org/10.3390/antiox9040270
Received: 26 February 2020 / Revised: 16 March 2020 / Accepted: 23 March 2020 / Published: 25 March 2020
(This article belongs to the Special Issue Natural Phenolic Compounds for Health, Food and Cosmetic Applications)
Eumelanins, the dark variant of skin pigments, are endowed with a remarkable antioxidant activity and well-recognized photoprotective properties that have been ascribed to pigment components derived from the biosynthetic precursor 5,6-dihydroxyindole-2-carboxylic acid (DHICA). Herein, we report the protective effect of a polymer obtained starting from the methyl ester of DHICA (MeDHICA-melanin) against Ultraviolet A (UVA)-induced oxidative stress in immortalized human keratinocytes (HaCaT). MeDHICA-melanin was prepared by aerial oxidation of MeDHICA. At concentrations as low as 10 µg/mL, MeDHICA-melanin prevented reactive oxygen species accumulation and partially reduced glutathione oxidation in UVA-irradiated keratinocytes. Western blot experiments revealed that the polymer is able to induce the translocation of nuclear factor erythroid 2–related factor 2 (Nrf-2) to the nucleus with the activation of the transcription of antioxidant enzymes, such as heme-oxygenase 1. Spectrophotometric and HPLC analysis of cell lysate allowed to conclude that a significant fraction (ca. 7%), consisting mainly of the 4,4′-dimer of MeDHICA (ca. 2 μM), was internalized in the cells. Overall these data point to the potential use of MeDHICA-melanin as an antioxidant for the treatment of skin damage, photoaging and skin cancers. View Full-Text
Keywords: melanins; 5,6-dihydroxyindole-2-carboxylic acid; antioxidant; photoprotection; UVA; HaCaT cells; reactive oxygen species; glutathione; Nrf-2 melanins; 5,6-dihydroxyindole-2-carboxylic acid; antioxidant; photoprotection; UVA; HaCaT cells; reactive oxygen species; glutathione; Nrf-2
Show Figures

Figure 1

MDPI and ACS Style

Liberti, D.; Alfieri, M.L.; Monti, D.M.; Panzella, L.; Napolitano, A. A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications. Antioxidants 2020, 9, 270. https://doi.org/10.3390/antiox9040270

AMA Style

Liberti D, Alfieri ML, Monti DM, Panzella L, Napolitano A. A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications. Antioxidants. 2020; 9(4):270. https://doi.org/10.3390/antiox9040270

Chicago/Turabian Style

Liberti, Davide; Alfieri, Maria L.; Monti, Daria M.; Panzella, Lucia; Napolitano, Alessandra. 2020. "A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications" Antioxidants 9, no. 4: 270. https://doi.org/10.3390/antiox9040270

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop