Next Article in Journal
The Promising Role of Antioxidant Phytochemicals in the Prevention and Treatment of Periodontal Disease via the Inhibition of Oxidative Stress Pathways: Updated Insights
Next Article in Special Issue
Chronic Metabolic Acidosis Elicits Hypertension via Upregulation of Intrarenal Angiotensin II and Induction of Oxidative Stress
Previous Article in Journal
Protein Carbonylation and Lipid Peroxidation in Hematological Malignancies
Previous Article in Special Issue
Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes
 
 
Article

Oxidized Low-Density Lipoprotein Associates with Ventricular Stress in Young Adults and Triggers Intracellular Ca2+ Alterations in Adult Ventricular Cardiomyocytes

1
Cardiorenal Translational Laboratory, Institute of Research i+12, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain
2
Department of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, 45004 Toledo, Spain
3
IBERMUTUA, 28043 Madrid, Spain
4
IdiPAZ Institute for Health Research/Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares, CIBER-CV, 28029 Madrid, Spain
5
Departament of Immunology, IIS-Fundación Jimenez Diaz, 28040 Madrid, Spain
6
Hypertension Unit, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain
7
CIBER-CV, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain
8
European University of Madrid, Madrid, Spain
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(12), 1213; https://doi.org/10.3390/antiox9121213
Received: 31 October 2020 / Revised: 20 November 2020 / Accepted: 26 November 2020 / Published: 1 December 2020
(This article belongs to the Special Issue Oxidative Stress and Inflammation in Cardiovascular Diseases)
Oxidized low-density lipoprotein (oxLDL) is associated with cardiac damage and causes injury to multiple cell types. We aimed to investigate the role of oxLDL in ventricular stress. We first examined the association between circulating oxLDL and N-terminal pro-brain natriuretic peptide (NT-proBNP), a marker of myocardial stress, in young subjects (30–50 years) with or without stable coronary artery disease (SCAD). oxLDL and NT-proBNP were significantly higher in subjects at high cardiovascular risk (CVR) than in subjects at low CVR and were associated independently of traditional CVR factors and C-reactive protein. Furthermore, the levels of oxLDL and NT-proBNP were significantly lower in subjects with SCAD than in peers at high CVR. To determine the intracellular mechanisms involved in the cardiac effects of oxLDL, we analyzed the in vitro effect of oxLDL on intracellular Ca2+ handling in adult rat ventricular cardiomyocytes using confocal microscopy. Acute challenge of adult ventricular cardiomyocytes to oxLDL reduced systolic Ca2+ transients and sarcoplasmic reticulum Ca2+ load. Moreover, diastolic spontaneous Ca2+ leak increased significantly after acute exposure to oxLDL. Thus, we demonstrate that oxLDL associates with NT-proBNP in young subjects, and can directly induce Ca2+ mishandling in adult ventricular cardiomyoyctes, predisposing cardiomyocytes to cardiac dysfunction and arrhythmogenicity. View Full-Text
Keywords: oxidized LDL; NT-proBNP; native adult ventricular cardiomyocytes; Ca2+ handling oxidized LDL; NT-proBNP; native adult ventricular cardiomyocytes; Ca2+ handling
Show Figures

Figure 1

MDPI and ACS Style

Rodríguez-Sánchez, E.; Navarro-García, J.A.; González-Lafuente, L.; Aceves-Ripoll, J.; Vázquez-Sánchez, S.; Poveda, J.; Mercado-García, E.; Corbacho-Alonso, N.; Calvo-Bonacho, E.; Fernández-Velasco, M.; Álvarez-Llamas, G.; Barderas, M.G.; Ruilope, L.M.; Ruiz-Hurtado, G. Oxidized Low-Density Lipoprotein Associates with Ventricular Stress in Young Adults and Triggers Intracellular Ca2+ Alterations in Adult Ventricular Cardiomyocytes. Antioxidants 2020, 9, 1213. https://doi.org/10.3390/antiox9121213

AMA Style

Rodríguez-Sánchez E, Navarro-García JA, González-Lafuente L, Aceves-Ripoll J, Vázquez-Sánchez S, Poveda J, Mercado-García E, Corbacho-Alonso N, Calvo-Bonacho E, Fernández-Velasco M, Álvarez-Llamas G, Barderas MG, Ruilope LM, Ruiz-Hurtado G. Oxidized Low-Density Lipoprotein Associates with Ventricular Stress in Young Adults and Triggers Intracellular Ca2+ Alterations in Adult Ventricular Cardiomyocytes. Antioxidants. 2020; 9(12):1213. https://doi.org/10.3390/antiox9121213

Chicago/Turabian Style

Rodríguez-Sánchez, Elena, José Alberto Navarro-García, Laura González-Lafuente, Jennifer Aceves-Ripoll, Sara Vázquez-Sánchez, Jonay Poveda, Elisa Mercado-García, Nerea Corbacho-Alonso, Eva Calvo-Bonacho, María Fernández-Velasco, Gloria Álvarez-Llamas, María G. Barderas, Luis M. Ruilope, and Gema Ruiz-Hurtado. 2020. "Oxidized Low-Density Lipoprotein Associates with Ventricular Stress in Young Adults and Triggers Intracellular Ca2+ Alterations in Adult Ventricular Cardiomyocytes" Antioxidants 9, no. 12: 1213. https://doi.org/10.3390/antiox9121213

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop