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Open AccessFeature PaperArticle

Biological Effects of Scattered Versus Scanned Proton Beams on Normal Tissues in Total Body Irradiated Mice: Survival, Genotoxicity, Oxidative Stress and Inflammation

1
Cancer Centre François Baclesse, Normandy University, UNICAEN, UNIROUEN, ABTE-EA4651, 14076 Caen, France
2
Translational Research Department, Experimental Radiotherapy Platform Institut Curie, PSL Research University, 91401 Orsay, France
3
Radiation Oncology Department, Proton Therapy Centre, Centre University, Institut Curie, PSL Research University, 91898 Orsay, France
4
Laboratoire d’Imagerie Translationnelle en Oncologie, INSERM, Institut Curie, PSL Research University, University Paris Saclay, 91401 Orsay, France
5
INSERM U1196/CNRS UMR 9187, Institut Curie-Recherche, University Paris-Saclay, 91405 Orsay, France
6
Radiotherapy Department, Cancer Centre François Baclesse, 14000 Caen, France
7
SAPHYN/ARCHADE (Advanced Resource Centre for HADrontherapy in Europe), 14000 Caen, France
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(12), 1170; https://doi.org/10.3390/antiox9121170
Received: 30 October 2020 / Revised: 16 November 2020 / Accepted: 18 November 2020 / Published: 24 November 2020
Side effects of proton therapy are poorly studied. Moreover, the differences in the method of dose delivery on normal tissues are not taken into account when proton beams are scanned instead of being scattered. We proposed here to study the effects of both modalities of proton beam delivery on blood; skin; lung and heart in a murine model. In that purpose; C57BL/6 mice were total body irradiated by 190.6 MeV proton beams either by Double Scattering (DS) or by Pencil Beam Scanning (PBS) in the plateau phase before the Bragg Peak. Mouse survival was evaluated. Blood and organs were removed three months after irradiation. Biomarkers of genotoxicity; oxidative stress and inflammation were measured. Proton irradiation was shown to increase lymphocyte micronucleus frequency; lung superoxide dismutase activity; erythrocyte and skin glutathione peroxidase activity; erythrocyte catalase activity; lung; heart and skin oxidized glutathione level; erythrocyte and lung lipid peroxidation and erythrocyte protein carbonylation even 3 months post-irradiation. When comparing both methods of proton beam delivery; mouse survival was not different. However, PBS significantly increased lymphocyte micronucleus frequency; erythrocyte glutathione peroxidase activity and heart oxidized glutathione level compared to DS. These results point out the necessity to take into account the way of delivering dose in PT as it could influence late side effects. View Full-Text
Keywords: proton therapy; double scattering; pencil beam scanning; side effects; healthy tissues; genotoxicity; oxidative stress; inflammation; mice proton therapy; double scattering; pencil beam scanning; side effects; healthy tissues; genotoxicity; oxidative stress; inflammation; mice
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MDPI and ACS Style

Chaouni, S.; Leduc, A.; Pouzoulet, F.; De Marzi, L.; Megnin-Chanet, F.; Stefan, D.; Habrand, J.-L.; Sichel, F.; Laurent, C. Biological Effects of Scattered Versus Scanned Proton Beams on Normal Tissues in Total Body Irradiated Mice: Survival, Genotoxicity, Oxidative Stress and Inflammation. Antioxidants 2020, 9, 1170.

AMA Style

Chaouni S, Leduc A, Pouzoulet F, De Marzi L, Megnin-Chanet F, Stefan D, Habrand J-L, Sichel F, Laurent C. Biological Effects of Scattered Versus Scanned Proton Beams on Normal Tissues in Total Body Irradiated Mice: Survival, Genotoxicity, Oxidative Stress and Inflammation. Antioxidants. 2020; 9(12):1170.

Chicago/Turabian Style

Chaouni, Samia; Leduc, Alexandre; Pouzoulet, Frédéric; De Marzi, Ludovic; Megnin-Chanet, Frédérique; Stefan, Dinu; Habrand, Jean-Louis; Sichel, François; Laurent, Carine. 2020. "Biological Effects of Scattered Versus Scanned Proton Beams on Normal Tissues in Total Body Irradiated Mice: Survival, Genotoxicity, Oxidative Stress and Inflammation" Antioxidants 9, no. 12: 1170.

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