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Open AccessArticle

Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line

1
Department of Biochemistry, Cell & Molecular Biology of Plants, Estación Experimental del Zaidín, Spanish National Research Council (CSIC), Profesor Albareda 1, Granada E-18008, Spain
2
Proteomic Research Department, San Cecilio University Hospital, Biosanitary Research Institute of Granada (Ibs.GRANADA), Av. Dr. Olóriz 16, Granada E-18012, Spain
3
Department of Physiology and Biochemistry of Animal Nutrition, Estación Experimental del Zaidín, Spanish National Research Council (CSIC), Camino del Jueves, Granada E-18100, Spain
4
The UWA Institute of Agriculture and School of Agriculture and Environment, The University of Western Australia, CRAWLEY Perth, WA 6019, Australia
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(1), 12; https://doi.org/10.3390/antiox9010012
Received: 3 November 2019 / Revised: 18 December 2019 / Accepted: 19 December 2019 / Published: 23 December 2019
(This article belongs to the Special Issue Antioxidants in Foods)
(1) Background: Inflammation molecular cues and insulin resistance development are some of the main contributors for the development and advance of the pathogenesis of inflammatory-related diseases; (2) Methods: We isolated and purified γ-conglutin protein from narrow-leafed lupin (NLL or blue lupin) mature seeds using affinity-chromatography to evaluate its anti-inflammatory activities at molecular level using both, a bacterial lipopolysaccharide (LPS)-induced inflammation and an insulin resistance pancreatic cell models; (3) Results: NLL γ-conglutin achieved a plethora of functional effects as the strong reduction of cell oxidative stress induced by inflammation through decreasing proteins carbonylation, nitric oxide synthesis and inducible nitric oxide synthase (iNOS) transcriptional levels, and raising glutathione (GSH) levels and modulation of superoxide dismutase (SOD) and catalase enzymes activities. γ-conglutin induced up-regulated transcriptomic and protein levels of insulin signalling pathway IRS-1, Glut-4, and PI3K, improving glucose uptake, while decreasing pro-inflammatory mediators as iNOs, TNFα, IL-1β, INFγ, IL-6, IL-12, IL-17, and IL-27; (4) Conclusion: These results suggest a promising use of NLL γ-conglutin protein in functional foods, which could also be implemented in alternative diagnosis and therapeutic molecular tools helping to prevent and treat inflammatory-related diseases. View Full-Text
Keywords: 7S basic globulins; anti-inflammatory protein; antioxidant protein; cytokines; glutathione; iNOS; nitric oxide; oxidative stress; sweet lupins group 7S basic globulins; anti-inflammatory protein; antioxidant protein; cytokines; glutathione; iNOS; nitric oxide; oxidative stress; sweet lupins group
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Lima-Cabello, E.; Alché, J.D.; Morales-Santana, S.; Clemente, A.; Jimenez-Lopez, J.C. Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line. Antioxidants 2020, 9, 12.

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