Modulation of Apoptotic Cell Death and Neuroprotective Effects of Glutathione—L-Dopa Codrug Against H2O2-Induced Cellular Toxicity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Treatment
2.2. Effects of Drugs on the Cytotoxicity of U937 Cell Supernatants
2.3. Cell Apoptosis Assessment
2.4. Western Blot Analysis
2.5. Caspase 3 Assay
2.6. ROS Detection
2.7. Activity of Antioxidant Enzymes
2.8. GSH Assay
2.9. Statistical Analysis
3. Results
3.1. Effect of GSH-LD on Cell Viability
3.2. Effect of GSH-LD on H2O2-Induced Apoptosis in Undifferentiated and Differentiated U937 Cells
3.3. Effect of GSH-LD on H2O2-Induced Apoptosis in Undifferentiated and Differentiated SH-SY5Y Cells
3.4. Effect of GSH-LD on the U937 Cell Mediated Cytotoxic in SH-SY5Y Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Franceschelli, S.; Lanuti, P.; Ferrone, A.; Gatta, D.M.P.; Speranza, L.; Pesce, M.; Grilli, A.; Cacciatore, I.; Ricciotti, E.; Di Stefano, A.; et al. Modulation of Apoptotic Cell Death and Neuroprotective Effects of Glutathione—L-Dopa Codrug Against H2O2-Induced Cellular Toxicity. Antioxidants 2019, 8, 319. https://doi.org/10.3390/antiox8080319
Franceschelli S, Lanuti P, Ferrone A, Gatta DMP, Speranza L, Pesce M, Grilli A, Cacciatore I, Ricciotti E, Di Stefano A, et al. Modulation of Apoptotic Cell Death and Neuroprotective Effects of Glutathione—L-Dopa Codrug Against H2O2-Induced Cellular Toxicity. Antioxidants. 2019; 8(8):319. https://doi.org/10.3390/antiox8080319
Chicago/Turabian StyleFranceschelli, Sara, Paola Lanuti, Alessio Ferrone, Daniela Maria Pia Gatta, Lorenza Speranza, Mirko Pesce, Alfredo Grilli, Ivana Cacciatore, Emanuela Ricciotti, Antonio Di Stefano, and et al. 2019. "Modulation of Apoptotic Cell Death and Neuroprotective Effects of Glutathione—L-Dopa Codrug Against H2O2-Induced Cellular Toxicity" Antioxidants 8, no. 8: 319. https://doi.org/10.3390/antiox8080319
APA StyleFranceschelli, S., Lanuti, P., Ferrone, A., Gatta, D. M. P., Speranza, L., Pesce, M., Grilli, A., Cacciatore, I., Ricciotti, E., Di Stefano, A., Miscia, S., Felaco, M., & Patruno, A. (2019). Modulation of Apoptotic Cell Death and Neuroprotective Effects of Glutathione—L-Dopa Codrug Against H2O2-Induced Cellular Toxicity. Antioxidants, 8(8), 319. https://doi.org/10.3390/antiox8080319