Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Chemicals
2.2. Preparation of Extract Fraction from F. Velutipes
2.3. Determination of Total Sugar, Uronic Acid, β-Glucan, and Protein Contents
2.4. Determination of Weight Average Molecular Weight
2.5. Monosaccharide Components Analysis
2.6. FT-IR Spectroscopic Analysis
2.7. Determination of Antioxidant Activities in Vitro
2.7.1. DPPH Radical Scavenging Assay
2.7.2. ABTS Radical Scavenging Assay
2.8. Cell Viability Assay
2.9. Intracellular ROS Content
2.10. Statistical Analysis
3. Results and Discussion
3.1. Physicochemical Properties of FVYs and FVWs
3.2. Monosaccharide Compositions analysis of FVYs and FVWs
3.3. FTIR Spectra of FVYs and FVWs
3.4. DPPH and ABTS Radical Scavenging Assay
3.5. Effect of FVYs and FVWs on Cell Viability and ROS Production of H2O2-Induced L929 Cells
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample | Total Sugar Content (%) | Uronic Acid Content (%) | β-Glucan Content (%) | Protein Content (%) |
---|---|---|---|---|
FVY-40 | 83.13 ± 1.29 Aa | 2.89 ± 0.36 Aa | 44.56 ± 1.77 Aa | 15.52 ± 3.14 Cb |
FVY-60 | 75.94 ± 5.03 ABa | 2.10 ± 017 Bb | 18.45 ± 0.49 Cb | 17.15 ± 3.45 Cb |
FVY-80 | 41.10 ± 2.09 Db | 1.71 ± 0.31 Bb | 16.68 ± 1.62 Cb | 35.85 ± 2.91 Aa |
FVW-40 | 77.99 ± 0.87 ABd | 2.02 ± 0.05 Bd | 44.18 ± 0.27 Ad | 14.16 ± 1.38 Ce |
FVW-60 | 60.54 ± 3.64 Ce | 1.81 ± 0.06 Be | 26.73 ± 2.11 Be | 23.60 ± 0.99 Bd |
FVW-80 | 40.87 ± 5.21 Df | 1.68 ± 0.14 Be | 13.55 ± 0.34 Df | 25.95 ± 0.25 Bd |
40% | 60% | 80% | |
---|---|---|---|
Yellow strain | 2377.04 kDa | 18.32 kDa | 3.84 kDa |
White strain | 2782.00 kDa | 19.09 kDa | 2.76 kDa |
Monosaccharide Composition | Yellow Strain | White Strain | |||||
---|---|---|---|---|---|---|---|
40% | 60% | 80% | 40% | 60% | 80% | ||
Glucose | 87.90 | 54.50 | 75.00 | 66.49 | 58.02 | 64.22 | |
Fructose | - | - | - | - | 0.25 | - | |
Molar | Mannose | 5.55 | 11.76 | 14.29 | 11.89 | 14.80 | 9.88 |
Percentage | Rhamnose | 0.81 | - | - | - | - | - |
(%) | Fucose | 1.36 | 11.90 | - | 3.73 | 7.24 | 13.55 |
Galactose | 2.59 | 21.03 | 10.71 | 15.37 | 18.79 | 12.35 | |
Arabinose | 0.29 | 0.82 | - | - | 0.10 | - | |
Xylose | 1.49 | - | - | 2.53 | 0.81 | - |
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Hu, Y.-N.; Sung, T.-J.; Chou, C.-H.; Liu, K.-L.; Hsieh, L.-P.; Hsieh, C.-W. Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell. Antioxidants 2019, 8, 298. https://doi.org/10.3390/antiox8080298
Hu Y-N, Sung T-J, Chou C-H, Liu K-L, Hsieh L-P, Hsieh C-W. Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell. Antioxidants. 2019; 8(8):298. https://doi.org/10.3390/antiox8080298
Chicago/Turabian StyleHu, Yu-Ning, Tzu-Jung Sung, Chun-Hsu Chou, Kai-Lun Liu, Liang-Po Hsieh, and Chang-Wei Hsieh. 2019. "Characterization and Antioxidant Activities of Yellow Strain Flammulina velutipes (Jinhua Mushroom) Polysaccharides and Their Effects on ROS Content in L929 Cell" Antioxidants 8, no. 8: 298. https://doi.org/10.3390/antiox8080298