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Open AccessArticle

Development and Validation of Conditions for Extracting Flavonoids Content and Evaluation of Antioxidant and Cytoprotective Activities from Bougainvillea x buttiana Bracteas (var. Rose)

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Facultad de Medicina de la, Universidad Autónoma del Estado de Morelos (UAEM), Calle: Leñeros, esquina Iztaccíhuatl s/n. Col. Volcanes, Cuernavaca, Mor. C.P. 62350, Mexico
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Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social (IMSS), Argentina No. 1, Xochitepec CP 62790, Morelos, Mexico
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(8), 264; https://doi.org/10.3390/antiox8080264
Received: 18 July 2019 / Revised: 26 July 2019 / Accepted: 31 July 2019 / Published: 1 August 2019
In this study the effect of the ethanol concentration of Bougainvillea x buttiana extracts on the flavonoids content, and its antioxidant and cytoprotective activities in vitro were determined and compared. For the elucidation of the chemical constituents, the high-performance liquid chromatography method (HPLC) was used, and verification of the antioxidant activity was carried out using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method. The cytoprotective effects of extracts were determined by exposure to hydrogen peroxide. The HPLC analysis showed the presence of rutin, quercetin-3-glucoside and quercetin rhamnoside. Among the extracts investigated the best recuperation of the rutin content was observed in extracts with 80% ethanol (83 ± 5 mg/mL). The amounts of rutin present in all extracts contribute to the antioxidant capacity and the IC50 was 427.49 (0%), 275.41 (50%), 271.61 (80%), and 272.14 (100%) µg/mL. The lowest percentage of viability was found in the cultures exposed to 100% ethanol (92%). In cultures exposed to hydrogen peroxide the percentages of protection were 25%, 33%, 78%, and 65% for cultures treated for 72 h at 0%, 50%, 80%, and 100% ethanol, respectively. The ethanolic extract of B. x buttiana was confirmed to have high rutin content with potent antioxidant activity, low cytotoxic and strong cytoprotective effects. View Full-Text
Keywords: rutin; quercetin-O-glucoside; quercetin-rhamnoside; extraction; Bougainvillea x buttiana; antioxidant activity; cytoprotective activity rutin; quercetin-O-glucoside; quercetin-rhamnoside; extraction; Bougainvillea x buttiana; antioxidant activity; cytoprotective activity
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Abarca-Vargas, R.; Zamilpa, A.; Petricevich, V.L. Development and Validation of Conditions for Extracting Flavonoids Content and Evaluation of Antioxidant and Cytoprotective Activities from Bougainvillea x buttiana Bracteas (var. Rose). Antioxidants 2019, 8, 264.

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