Protein Redox State Monitoring Studies of Thiol Reactivity
Abstract
1. Introduction
2. Methods
2.1. Protein Expression and Purification
2.2. Cell Culture
2.3. Protein Redox State Monitoring
2.4. Statistical Analysis
3. Results
3.1. -SulfoBiotics- Protein Redox State Monitoring Kit to Assess Thiol States of Purified Proteins
3.2. Redox Studies of Prx6 in Cultured Cells Using -Sulfobiotics- Protein Redox State Monitoring Kit Plus
3.3. H2O2 Can Decrease Disulfide Bonds in the Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Suzuki, Y.J.; Marcocci, L.; Shimomura, T.; Tatenaka, Y.; Ohuchi, Y.; Brelidze, T.I. Protein Redox State Monitoring Studies of Thiol Reactivity. Antioxidants 2019, 8, 143. https://doi.org/10.3390/antiox8050143
Suzuki YJ, Marcocci L, Shimomura T, Tatenaka Y, Ohuchi Y, Brelidze TI. Protein Redox State Monitoring Studies of Thiol Reactivity. Antioxidants. 2019; 8(5):143. https://doi.org/10.3390/antiox8050143
Chicago/Turabian StyleSuzuki, Yuichiro J., Lucia Marcocci, Takashi Shimomura, Yuki Tatenaka, Yuya Ohuchi, and Tinatin I. Brelidze. 2019. "Protein Redox State Monitoring Studies of Thiol Reactivity" Antioxidants 8, no. 5: 143. https://doi.org/10.3390/antiox8050143
APA StyleSuzuki, Y. J., Marcocci, L., Shimomura, T., Tatenaka, Y., Ohuchi, Y., & Brelidze, T. I. (2019). Protein Redox State Monitoring Studies of Thiol Reactivity. Antioxidants, 8(5), 143. https://doi.org/10.3390/antiox8050143