Induction of Antioxidant Protein HO-1 Through Nrf2-ARE Signaling Due to Pteryxin in Peucedanum Japonicum Thunb in RAW264.7 Macrophage Cells
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Isolation of Pteryxin
2.3. Analysis of Pteryxin
2.4. Cell Culture
2.5. Cell Survaival
2.6. Activity of Nrf2-ARE Signaling
2.7. Nuclear Extraction and Determination of Nrf2
2.8. Protein Expression by Western Blot Analysis
3. Results
3.1. Determination of Pteryxin
3.2. Nrf2-ARE Signaling
3.3. Nrf2 Expression in Cytoplasm and Nuclei
3.4. HO-1 Expression
3.5. Nrf2-ARE Signaling Activity and HO-1 Protein Expression by Coumarin Derivatives
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Taira, J.; Ogi, T. Induction of Antioxidant Protein HO-1 Through Nrf2-ARE Signaling Due to Pteryxin in Peucedanum Japonicum Thunb in RAW264.7 Macrophage Cells. Antioxidants 2019, 8, 621. https://doi.org/10.3390/antiox8120621
Taira J, Ogi T. Induction of Antioxidant Protein HO-1 Through Nrf2-ARE Signaling Due to Pteryxin in Peucedanum Japonicum Thunb in RAW264.7 Macrophage Cells. Antioxidants. 2019; 8(12):621. https://doi.org/10.3390/antiox8120621
Chicago/Turabian StyleTaira, Junsei, and Takayuki Ogi. 2019. "Induction of Antioxidant Protein HO-1 Through Nrf2-ARE Signaling Due to Pteryxin in Peucedanum Japonicum Thunb in RAW264.7 Macrophage Cells" Antioxidants 8, no. 12: 621. https://doi.org/10.3390/antiox8120621
APA StyleTaira, J., & Ogi, T. (2019). Induction of Antioxidant Protein HO-1 Through Nrf2-ARE Signaling Due to Pteryxin in Peucedanum Japonicum Thunb in RAW264.7 Macrophage Cells. Antioxidants, 8(12), 621. https://doi.org/10.3390/antiox8120621