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Open AccessArticle

Isolation of Phytochemicals from Bauhinia variegata L. Bark and Their In Vitro Antioxidant and Cytotoxic Potential

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Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, India
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State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China
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Department of Biophysics, Panjab University, Chandigarh 160014, India
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Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar 143005, India
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Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, Italy
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Department of Plant Physiology, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra 94976, Slovakia
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Department of Botany and Plant Physiology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 16500 Prague, Czech Republic
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Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, India
*
Authors to whom correspondence should be addressed.
Antioxidants 2019, 8(10), 492; https://doi.org/10.3390/antiox8100492
Received: 6 September 2019 / Revised: 7 October 2019 / Accepted: 16 October 2019 / Published: 17 October 2019
(This article belongs to the Special Issue Phenolic Profiling and Antioxidant Capacity in Plants)
Plants have been the basis of traditional medicine since the dawn of civilizations. Different plant parts possess various phytochemicals, playing important roles in preventing and curing diseases. Scientists, through extensive experimental studies, are playing an important part in establishing the use of phytochemicals in medicine. However, there are still a large number of medicinal plants which need to be studied for their phytochemical profile. In this study, the objective was to isolate phytochemicals from bark of Bauhinia variegata L. and to study them for their antioxidant and cytotoxic activities. The bark was extracted with methanol, followed by column chromatography and thus isolating kaempferol, stigmasterol, protocatechuic acid-methyl ester (PCA-ME) and protocatechuic acid (PCA). 2,2-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 2, 2’-diphenyl-1-picrylhydrazyl radical (DPPH) radical scavenging assays were utilized for assessment of antioxidant activity, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dye reduction assay was used to determine cytotoxic activity against C-6 glioma rat brain, MCF-7 breast cancer, and HCT-15 colon cancer cell lines. The compounds were found to have significant antioxidant and cytotoxic activity. Since there is a considerable increase in characterizing novel chemical compounds from plant parts, the present study might be helpful for chemotaxonomic determinations, for understanding of medicinal properties as well as for the quality assessment of herbal supplements containing B. variegata bark, thus establishing its use in traditional medicine. View Full-Text
Keywords: antioxidative system; anti-cancer compounds; oxidative stress; polyphenols; phytochemicals antioxidative system; anti-cancer compounds; oxidative stress; polyphenols; phytochemicals
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Sharma, N.; Sharma, A.; Bhatia, G.; Landi, M.; Brestic, M.; Singh, B.; Singh, J.; Kaur, S.; Bhardwaj, R. Isolation of Phytochemicals from Bauhinia variegata L. Bark and Their In Vitro Antioxidant and Cytotoxic Potential. Antioxidants 2019, 8, 492.

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