Next Article in Journal / Special Issue
Berry Fruit Consumption and Metabolic Syndrome
Previous Article in Journal
A Review of Antioxidant Peptides Derived from Meat Muscle and By-Products
Previous Article in Special Issue
Cranberries and Cancer: An Update of Preclinical Studies Evaluating the Cancer Inhibitory Potential of Cranberry and Cranberry Derived Constituents
Open AccessArticle

Tart Cherry Extracts Reduce Inflammatory and Oxidative Stress Signaling in Microglial Cells

USDA-ARS, Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, MA 02111, USA
Author to whom correspondence should be addressed.
Academic Editor: Dorothy Klimis-Zacas
Antioxidants 2016, 5(4), 33;
Received: 7 July 2016 / Revised: 2 September 2016 / Accepted: 9 September 2016 / Published: 22 September 2016
(This article belongs to the Special Issue Berry Antioxidants in Health and Disease)
Tart cherries contain an array of polyphenols that can decrease inflammation and oxidative stress (OS), which contribute to cognitive declines seen in aging populations. Previous studies have shown that polyphenols from dark-colored fruits can reduce stress-mediated signaling in BV-2 mouse microglial cells, leading to decreases in nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression. Thus, the present study sought to determine if tart cherries—which improved cognitive behavior in aged rats—would be efficacious in reducing inflammatory and OS signaling in HAPI rat microglial cells. Cells were pretreated with different concentrations (0–1.0 mg/mL) of Montmorency tart cherry powder for 1–4 h, then treated with 0 or 100 ng/mL lipopolysaccharide (LPS) overnight. LPS application increased extracellular levels of NO and tumor necrosis factor-alpha (TNF-α), and intracellular levels of iNOS and cyclooxygenase-2 (COX-2). Pretreatment with tart cherry decreased levels of NO, TNF-α, and COX-2 in a dose- and time-dependent manner versus those without pretreatment; the optimal combination was between 0.125 and 0.25 mg/mL tart cherry for 2 h. Higher concentrations of tart cherry powder and longer exposure times negatively affected cell viability. Therefore, tart cherries (like other dark-colored fruits), may be effective in reducing inflammatory and OS-mediated signals. View Full-Text
Keywords: antioxidant; anti-inflammatory; polyphenols; anthocyanins; cytokines antioxidant; anti-inflammatory; polyphenols; anthocyanins; cytokines
Show Figures

Figure 1

MDPI and ACS Style

Shukitt-Hale, B.; Kelly, M.E.; Bielinski, D.F.; Fisher, D.R. Tart Cherry Extracts Reduce Inflammatory and Oxidative Stress Signaling in Microglial Cells. Antioxidants 2016, 5, 33.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop