Epigallocatechin-3-Gallate Attenuates Myocardial Dysfunction via Inhibition of Endothelial-to-Mesenchymal Transition
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Cell Culture and Treatment
2.3. Cell Viability Assay
2.4. Western Blot Assay
2.5. Wound Healing Analysis
2.6. ROS Measurement
2.7. Mitochondrial Membrane Potential Assay
2.8. LDL Uptake Assay
2.9. Permeability Assay
2.10. Real-Time PCR
2.11. MI Model and EchA Treatment
2.12. 2,3,5-Triphenyltetrazolium chloride (TTC) Staining
2.13. Histology and Immunofluorescence
2.14. Cardiac Catheterization
2.15. Statistical Analysis
3. Results
3.1. EGCG Inhibits EndMT to Maintain Endothelial Cell Properties
3.2. EGCG Inhibits Cell Migration by Affecting the RhoA GTPase Pathway
3.3. EGCG Reduces Oxidative Stress and Regulates NF-κB and SMAD Signaling Pathways
3.4. EGCG Regulates the EndMT Process in Ischemic Myocardium
3.5. EGCG Improves Cardiac Function by Controlling the EndMT Process in Ischemic Heart Tissue
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, S.; Lee, H.; Moon, H.; Kim, R.; Kim, M.; Jeong, S.; Kim, H.; Kim, S.H.; Hwang, S.S.; Lee, M.Y.; et al. Epigallocatechin-3-Gallate Attenuates Myocardial Dysfunction via Inhibition of Endothelial-to-Mesenchymal Transition. Antioxidants 2023, 12, 1059. https://doi.org/10.3390/antiox12051059
Kim S, Lee H, Moon H, Kim R, Kim M, Jeong S, Kim H, Kim SH, Hwang SS, Lee MY, et al. Epigallocatechin-3-Gallate Attenuates Myocardial Dysfunction via Inhibition of Endothelial-to-Mesenchymal Transition. Antioxidants. 2023; 12(5):1059. https://doi.org/10.3390/antiox12051059
Chicago/Turabian StyleKim, Sejin, Hyunjae Lee, Hanbyeol Moon, Ran Kim, Minsuk Kim, Seongtae Jeong, Hojin Kim, Sang Hyeon Kim, Soo Seok Hwang, Min Young Lee, and et al. 2023. "Epigallocatechin-3-Gallate Attenuates Myocardial Dysfunction via Inhibition of Endothelial-to-Mesenchymal Transition" Antioxidants 12, no. 5: 1059. https://doi.org/10.3390/antiox12051059
APA StyleKim, S., Lee, H., Moon, H., Kim, R., Kim, M., Jeong, S., Kim, H., Kim, S. H., Hwang, S. S., Lee, M. Y., Kim, J., Song, B.-W., & Chang, W. (2023). Epigallocatechin-3-Gallate Attenuates Myocardial Dysfunction via Inhibition of Endothelial-to-Mesenchymal Transition. Antioxidants, 12(5), 1059. https://doi.org/10.3390/antiox12051059