Systematical Characterization of the Cotton Di19 Gene Family and the Role of GhDi19-3 and GhDi19-4 as Two Negative Regulators in Response to Salt Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Sequence Identification and Phylogenetic Analysis
2.3. Sequence Characteristics and Chromosome Distribution of Di19s
2.4. Transcriptomic Data and Expression Patterns Analysis of Di19s
2.5. Protein Association Network of GhDi19s
2.6. Subcellular Localization of GhDi19-3 and GhDi19-4 in Tobacco
2.7. VIGS Vector Construction and Treatments
2.8. Detection and Analysis of Salt Stress-Related Physiological Parameters
3. Results
3.1. Identification and Characterization of Di19 Genes
3.2. Phylogenetics, Sequence Structure, and Conserved Motif of the Di19s
3.3. Conserved Cis-Elements and Expression Profiles of GhDi19 Genes
3.4. Protein Association Network
3.5. Subcellular Localization of GhDi19-3 and GhDi19-4 Proteins
3.6. Silencing of GhDi19-3 and GhDi19-4 Increases the Tolerance of Cotton to Salt Stress
3.7. Expression Profiling of Calcium Signaling-Related and ABA-Responsive Genes in Simulated Salt Stress Treatment of Control and GhDi19-3- and GhDi19-4-Silenced Cotton Plants
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, L.; Li, Y.; Li, Y.; Chen, W.; Yao, J.; Fang, S.; Lv, Y.; Zhang, Y.; Zhu, S. Systematical Characterization of the Cotton Di19 Gene Family and the Role of GhDi19-3 and GhDi19-4 as Two Negative Regulators in Response to Salt Stress. Antioxidants 2022, 11, 2225. https://doi.org/10.3390/antiox11112225
Zhao L, Li Y, Li Y, Chen W, Yao J, Fang S, Lv Y, Zhang Y, Zhu S. Systematical Characterization of the Cotton Di19 Gene Family and the Role of GhDi19-3 and GhDi19-4 as Two Negative Regulators in Response to Salt Stress. Antioxidants. 2022; 11(11):2225. https://doi.org/10.3390/antiox11112225
Chicago/Turabian StyleZhao, Lanjie, Youzhong Li, Yan Li, Wei Chen, Jinbo Yao, Shengtao Fang, Youjun Lv, Yongshan Zhang, and Shouhong Zhu. 2022. "Systematical Characterization of the Cotton Di19 Gene Family and the Role of GhDi19-3 and GhDi19-4 as Two Negative Regulators in Response to Salt Stress" Antioxidants 11, no. 11: 2225. https://doi.org/10.3390/antiox11112225