ROS-Responsive and pH-Sensitive Aminothiols Dual-Prodrug for Radiation Enteritis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Characterization
2.3. Synthesis and Characterization of M1
2.4. Cell Culture and Cytotoxicity Evaluation
2.5. Irradiation Conditions
2.6. Sulfhydryl Group Detection
2.7. ROS Detection
2.8. Colony Formation Assay
2.9. Apoptosis Assays
2.10. Alkaline Comet Assay
2.11. Detection of γ-H2AX
2.12. Mice
2.13. Histology
2.14. Immunohistochemical Analysis
2.15. Statistical Analysis
3. Results
3.1. Solution Characterization of M1 on pH-Sensitive and ROS-Responsive
3.2. M1 Inhibits the Reduction in Clone Formation by Scavenging ROS
3.3. M1 Ameliorates Apoptosis Induced by Irradiation
3.4. M1 Attenuates Radiation-Induced DNA Damage
3.5. M1 Ameliorates Intestine Structural Injuries Induced by 13 Gy ABI
3.6. M1 Improves Cell Proliferation, Reduces Cell Apoptosis, and Enhances Epithelial Integrity in Intestinal Crypts after 13 Gy ABI
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chen, Y.; Yang, Y.; Tang, H.; Zhang, Z.; Zhou, X.; Xu, W. ROS-Responsive and pH-Sensitive Aminothiols Dual-Prodrug for Radiation Enteritis. Antioxidants 2022, 11, 2145. https://doi.org/10.3390/antiox11112145
Chen Y, Yang Y, Tang H, Zhang Z, Zhou X, Xu W. ROS-Responsive and pH-Sensitive Aminothiols Dual-Prodrug for Radiation Enteritis. Antioxidants. 2022; 11(11):2145. https://doi.org/10.3390/antiox11112145
Chicago/Turabian StyleChen, Yuanfang, Yuwei Yang, Haikang Tang, Ziqi Zhang, Xiaoliang Zhou, and Wenqing Xu. 2022. "ROS-Responsive and pH-Sensitive Aminothiols Dual-Prodrug for Radiation Enteritis" Antioxidants 11, no. 11: 2145. https://doi.org/10.3390/antiox11112145