Next Article in Journal
Agaricales Mushroom Lignin Peroxidase: From Structure–Function to Degradative Capabilities
Next Article in Special Issue
Salivary Oxidative Stress Markers’ Relation to Oral Diseases in Children and Adolescents
Previous Article in Journal
Mitochondrial Oxidation of the Cytoplasmic Reducing Equivalents at the Onset of Oxidant Stress in the Isoproterenol-Induced Rat Myocardial Infarction
Previous Article in Special Issue
The Influence of Oxidative Stress on Thyroid Diseases
Article

Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway

by 1,2,†, 3,4,5,†, 6 and 6,*
1
Department of Urology, E-Da Hospital, Kaohsiung 82445, Taiwan
2
Department of Medical Laboratory Science and Biotechnology, Chung-Hwa University of Medical Technology, Tainan 71703, Taiwan
3
Institute of Oral Sciences, Chung Shan Medical University, Taichung 40201, Taiwan
4
Department of Dentistry, Chung Shan Medical University Hospital, Taichung 40201, Taiwan
5
School of Dentistry, Chung Shan Medical University, Taichung 40201, Taiwan
6
Department of Anatomy, School of Medicine, China Medical University, Taichung 404333, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Soliman Khatib and Dana Atrahimovich Blatt
Antioxidants 2021, 10(9), 1445; https://doi.org/10.3390/antiox10091445
Received: 24 August 2021 / Revised: 7 September 2021 / Accepted: 9 September 2021 / Published: 12 September 2021
(This article belongs to the Special Issue Oxidative-Stress in Human Diseases)
Impaired wound healing often occurs in patients with diabetes and causes great inconvenience to them. Aside from the presence of prolonged inflammation, the accumulation of oxidative stress is also implicated in the delayed wound healing. In the present study, we tested the effect of verbascoside, a caffeoyl phenylethanoid glycoside, on the improvement of cell viability and wound healing capacity of gingival epithelial cells under high glucose condition. We showed that verbascoside attenuated the high glucose-induced cytotoxicity and impaired healing, which may be associated with the downregulation of oxidative stress. Our results demonstrated that verbascoside increased the activity of the antioxidant enzyme SOD and reduced the oxidative stress indicator, 8-OHdG, as well as apoptosis. Moreover, verbascoside upregulated the PGC1-α and NRF1 expression and promoted mitochondrial biogenesis, which was mediated by suppression of PKC/HMGB1/RAGE/NFκB signaling. Likewise, we showed the inhibitory effect of verbascoside on oxidative stress was via repression of PKC/HMGB1/RAGE/NFκB activation. Also, our data suggested that the PKC-mediated oxidative stress may lead to the elevated production of inflammatory cytokines, IL-6 and IL-1β. Collectively, we demonstrated that verbascoside may be beneficial to ameliorate impaired oral wound healing for diabetic patients. View Full-Text
Keywords: verbascoside; PKC; HMGB1; impaired wound healing; antioxidant activity verbascoside; PKC; HMGB1; impaired wound healing; antioxidant activity
Show Figures

Figure 1

MDPI and ACS Style

Hsieh, P.-F.; Yu, C.-C.; Chu, P.-M.; Hsieh, P.-L. Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway. Antioxidants 2021, 10, 1445. https://doi.org/10.3390/antiox10091445

AMA Style

Hsieh P-F, Yu C-C, Chu P-M, Hsieh P-L. Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway. Antioxidants. 2021; 10(9):1445. https://doi.org/10.3390/antiox10091445

Chicago/Turabian Style

Hsieh, Pei-Fang, Cheng-Chia Yu, Pei-Ming Chu, and Pei-Ling Hsieh. 2021. "Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway" Antioxidants 10, no. 9: 1445. https://doi.org/10.3390/antiox10091445

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop