Next Article in Journal
Tanshinone IIA Stimulates Cystathionine γ-Lyase Expression and Protects Endothelial Cells from Oxidative Injury
Previous Article in Journal
Impact of Protein-Enriched Plant Food Items on the Bioaccessibility and Cellular Uptake of Carotenoids
Article

Mint Oils: In Vitro Ability to Perform Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and to Enhance Intestinal Barrier Integrity

1
Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, Via Trentacoste 2, 20134 Milan, Italy
2
Department of Animal Science, University of California, Davis, 4302 Meyer Hall, One Shields Ave, Davis, CA 95616, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Jose Manuel Lorenzo Rodriguez
Antioxidants 2021, 10(7), 1004; https://doi.org/10.3390/antiox10071004
Received: 24 May 2021 / Revised: 16 June 2021 / Accepted: 21 June 2021 / Published: 23 June 2021
The objectives of the study were to test the biological activities of peppermint and spearmint oils via (i) measuring in vitro anti-inflammatory effects with porcine alveolar macrophages (PAMs), (ii) determining the barrier integrity of IPEC-J2 by analyzing transepithelial electrical resistance (TEER), (iii) testing their antioxidant activities, and (iv) investigating the antimicrobial activity against enterotoxigenic Escherichia coli (ETEC) F18+. Briefly, (i) macrophages were seeded at 106 cells/mL and treated (24 h) with mint oils and lipopolysaccharide (LPS). The treatments were 2 (0 or 1 μg/mL of LPS) × 5 (0, 25, 50, 100, 200 µg/mL of mint oils). The supernatants were collected for TNF-α and IL-1β measurement by ELISA; (ii) IPEC-J2 cells were seeded at 5 × 105 cells/mL and treated with mint oils (0, 25, 50, 100, and 200 μg/mL). TEER (Ωcm2) was measured at 0, 24, 48, and 72 h; (iii) the antioxidant activity was assessed (0, 1, 50, 100, 200, 500, and 600 mg/mL) using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reducing power assays; (iv) overnight-grown ETEC F18+ were quantified (CFU/mL) after supplementing with peppermint and spearmint oils (0, 1.44, 2.87, 5.75, 11.50, and 23.00 mg/mL). All data were analyzed using the MIXED procedure. Both mint oils significantly inhibited (p < 0.05) IL-1β and TNF-α secretion from LPS-stimulated PAMs. Mint oil treatments did not affect TEER in IPEC-J2. Spearmint and peppermint oils exhibited (p < 0.05) strong antioxidant activities in DPPH and reducing power assays. Both mint oils also dose-dependently inhibited (p < 0.05) the growth of ETEC F18+ in vitro. The results of the study indicated that both mint oils are great candidate feed additives due to their in vitro anti-inflammatory, antioxidant, and antimicrobial effects. Further research is needed to evaluate their efficacy in vivo. View Full-Text
Keywords: anti-inflammatory; antioxidant; antimicrobial; mint oils; pigs; transepithelial electrical resistance anti-inflammatory; antioxidant; antimicrobial; mint oils; pigs; transepithelial electrical resistance
Show Figures

Figure 1

MDPI and ACS Style

Hejna, M.; Kovanda, L.; Rossi, L.; Liu, Y. Mint Oils: In Vitro Ability to Perform Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and to Enhance Intestinal Barrier Integrity. Antioxidants 2021, 10, 1004. https://doi.org/10.3390/antiox10071004

AMA Style

Hejna M, Kovanda L, Rossi L, Liu Y. Mint Oils: In Vitro Ability to Perform Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and to Enhance Intestinal Barrier Integrity. Antioxidants. 2021; 10(7):1004. https://doi.org/10.3390/antiox10071004

Chicago/Turabian Style

Hejna, Monika, Lauren Kovanda, Luciana Rossi, and Yanhong Liu. 2021. "Mint Oils: In Vitro Ability to Perform Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and to Enhance Intestinal Barrier Integrity" Antioxidants 10, no. 7: 1004. https://doi.org/10.3390/antiox10071004

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop