Next Article in Journal
Oxidative Stress Mediates Vascular Tortuosity
Next Article in Special Issue
The Multifaceted Role of Flavonoids in Cancer Therapy: Leveraging Autophagy with a Double-Edged Sword
Previous Article in Journal
Coenzyme Q10 Phytosome Formulation Improves CoQ10 Bioavailability and Mitochondrial Functionality in Cultured Cells
Previous Article in Special Issue
Apigenin and Luteolin Regulate Autophagy by Targeting NRH-Quinone Oxidoreductase 2 in Liver Cells
Article

Green Tea Polyphenol Catechins Inhibit Coronavirus Replication and Potentiate the Adaptive Immunity and Autophagy-Dependent Protective Mechanism to Improve Acute Lung Injury in Mice

1
Department of Life Science, School of Life Science, College of Science, National Taiwan Normal University, Taipei 11677, Taiwan
2
Office of Public Relation of Ministry of Health and Welfare, Taipei 115204, Taiwan
3
Center for General Education, Mackay College of Medicine, Nursing and Management, New Taipei City 11260, Taiwan
4
Department of Food Science, Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202301, Taiwan
5
Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan
6
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
7
Mackay Junior College of Medicine, Nursing and Management, New Taipei City 11260, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Gregory Quinlan
Antioxidants 2021, 10(6), 928; https://doi.org/10.3390/antiox10060928
Received: 21 April 2021 / Revised: 1 June 2021 / Accepted: 4 June 2021 / Published: 7 June 2021
Effective antiviral therapeutics are urgently required to fight severe acute respiratory syndrome (SARS) caused by a SARS coronavirus (SARS-CoV). Because polyphenol catechins could confer antioxidative, anti-inflammatory, antiviral, and antimicrobial activities, we assessed the therapeutic effects of catechins against SARS-CoV replication in Vero E6 cells, the preventive effect of catechins on CD25/CD69/CD94/CD8+ cytotoxic T lymphocytes-mediated adaptive immunity, and the protective effect on lipopolysaccharide-induced acute lung injury (ALI) in mice. We found that catechins containing 32.8% epigallocatechin gallate, 15.2% epicatechin gallate, 13.2 epicatechin, 10.8% epigallocatechin, 10.4% gallocatechin, and 4.4% catechin directly inhibited SARS-CoV replication at sub-micromolecular concentrations. Four-week catechins ingestion increased CD8+ T cell percentage, upregulated CD69+/CD25+/CD94-NKG2A/CD8+ T lymphocytes-mediated adaptive immunity, and increased type I cytokines release responding to ovalbumin/alum. Catechins significantly reduced lipopolysaccharide-induced cytokine storm and oxidative stress and ALI by inhibiting PI3K/AKT/mTOR signaling to upregulate Beclin-1/Atg5-Atg12/LC3-II-mediated autophagy mechanism. Pretreatment of autophagy inhibitor 3-Methyladenine reversed the inhibiting effects of catechins on the cytokines and oxidative stress levels and ALI. In conclusion, our data indicated that catechins directly inhibited SARS-CoV replication, potentiated the CD25/CD69/CD94/CD8+ T lymphocytes-mediated adaptive immunity and attenuated lipopolysaccharide-induced ALI and cytokine storm by PI3K/AKT/mTOR-signaling-mediated autophagy, which may be applied to prevent and/or treat SARS-CoV infection. View Full-Text
Keywords: acute lung injury; adaptive immunity; autophagy; catechins; cytokine storm; SARS-CoV replication acute lung injury; adaptive immunity; autophagy; catechins; cytokine storm; SARS-CoV replication
Show Figures

Graphical abstract

MDPI and ACS Style

Yang, C.-C.; Wu, C.-J.; Chien, C.-Y.; Chien, C.-T. Green Tea Polyphenol Catechins Inhibit Coronavirus Replication and Potentiate the Adaptive Immunity and Autophagy-Dependent Protective Mechanism to Improve Acute Lung Injury in Mice. Antioxidants 2021, 10, 928. https://doi.org/10.3390/antiox10060928

AMA Style

Yang C-C, Wu C-J, Chien C-Y, Chien C-T. Green Tea Polyphenol Catechins Inhibit Coronavirus Replication and Potentiate the Adaptive Immunity and Autophagy-Dependent Protective Mechanism to Improve Acute Lung Injury in Mice. Antioxidants. 2021; 10(6):928. https://doi.org/10.3390/antiox10060928

Chicago/Turabian Style

Yang, Chih-Ching, Chang-Jer Wu, Chen-Yen Chien, and Chiang-Ting Chien. 2021. "Green Tea Polyphenol Catechins Inhibit Coronavirus Replication and Potentiate the Adaptive Immunity and Autophagy-Dependent Protective Mechanism to Improve Acute Lung Injury in Mice" Antioxidants 10, no. 6: 928. https://doi.org/10.3390/antiox10060928

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop