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Open AccessArticle

Blunted Reducing Power Generation in Erythrocytes Contributes to Oxidative Stress in Prepubertal Obese Children with Insulin Resistance

1
Inflammation, Nutrition, Metabolism and Oxidative Stress Study Group (INMOX), Biomedical Research and Innovation Institute of Cádiz (INiBICA), Research Unit, Puerta del Mar University Hospital, 11009 Cádiz, Spain
2
Pediatric Endocrinology and Diabetes, Department of Pediatrics, Puerta del Mar University Hospital, 11009 Cádiz, Spain
3
Clinical Analysis Department, Puerta del Mar University Hospital, 11009 Cádiz, Spain
4
Area of Pediatrics, Department of Child and Mother Health and Radiology, Medical School, University of Cádiz, 11003 Cádiz, Spain
5
Area of Biochemistry and Molecular Biology, Department of Biomedicine, Biotechnology and Public Health, University of Cádiz, 11519 Cádiz, Spain
*
Authors to whom correspondence should be addressed.
Both authors contributed equally.
Academic Editors: Mario Allegra and Josep A. Tur
Antioxidants 2021, 10(2), 244; https://doi.org/10.3390/antiox10020244
Received: 23 December 2020 / Revised: 29 January 2021 / Accepted: 1 February 2021 / Published: 5 February 2021
Childhood obesity, and specifically its metabolic complications, are related to deficient antioxidant capacity and oxidative stress. Erythrocytes are constantly exposed to multiple sources of oxidative stress; hence, they are equipped with powerful antioxidant mechanisms requiring permanent reducing power generation and turnover. Glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) are two key enzymes on the pentose phosphate pathway. Both enzymes supply reducing power by generating NADPH, which is essential for maintaining the redox balance within the cell and the activity of other antioxidant enzymes. We hypothesized that obese children with insulin resistance would exhibit blunted G6PDH and 6PGDH activities, contributing to their erythrocytes’ redox status imbalances. We studied 15 control and 24 obese prepubertal children, 12 of whom were insulin-resistant according to an oral glucose tolerance test (OGTT). We analyzed erythroid malondialdehyde (MDA) and carbonyl group levels as oxidative stress markers. NADP+/NADPH and GSH/GSSG were measured to determine redox status, and NADPH production by both G6PDH and 6PGDH was assayed spectrophotometrically to characterize pentose phosphate pathway activity. Finally, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) activities were also assessed. As expected, MDA and carbonyl groups levels were higher at baseline and along the OGTT in insulin-resistant children. Both redox indicators showed an imbalance in favor of the oxidized forms along the OGTT in the insulin-resistant obese group. Additionally, the NADPH synthesis, as well as GR activity, were decreased. H2O2 removing enzyme activities were depleted at baseline in both obese groups, although after sugar intake only metabolically healthy obese participants were able to maintain their catalase activity. No change was detected in SOD activity between groups. Our results show that obese children with insulin resistance present higher levels of oxidative damage, blunted capacity to generate reducing power, and hampered function of key NADPH-dependent antioxidant enzymes. View Full-Text
Keywords: antioxidants; childhood obesity; insulin resistance; oxidative stress; pentose phosphate pathway; reducing power antioxidants; childhood obesity; insulin resistance; oxidative stress; pentose phosphate pathway; reducing power
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MDPI and ACS Style

González-Domínguez, Á.; Visiedo, F.; Domínguez-Riscart, J.; Ruiz-Mateos, B.; Saez-Benito, A.; Lechuga-Sancho, A.M.; Mateos, R.M. Blunted Reducing Power Generation in Erythrocytes Contributes to Oxidative Stress in Prepubertal Obese Children with Insulin Resistance. Antioxidants 2021, 10, 244. https://doi.org/10.3390/antiox10020244

AMA Style

González-Domínguez Á, Visiedo F, Domínguez-Riscart J, Ruiz-Mateos B, Saez-Benito A, Lechuga-Sancho AM, Mateos RM. Blunted Reducing Power Generation in Erythrocytes Contributes to Oxidative Stress in Prepubertal Obese Children with Insulin Resistance. Antioxidants. 2021; 10(2):244. https://doi.org/10.3390/antiox10020244

Chicago/Turabian Style

González-Domínguez, Álvaro; Visiedo, Francisco; Domínguez-Riscart, Jesus; Ruiz-Mateos, Beatriz; Saez-Benito, Ana; Lechuga-Sancho, Alfonso M.; Mateos, Rosa M. 2021. "Blunted Reducing Power Generation in Erythrocytes Contributes to Oxidative Stress in Prepubertal Obese Children with Insulin Resistance" Antioxidants 10, no. 2: 244. https://doi.org/10.3390/antiox10020244

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