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Article

Effect of Young Plasma Therapy on Cognition, Oxidative Stress, miRNA-134, BDNF, CREB, and SIRT-1 Expressions and Neuronal Survey in the Hippocampus of Aged Ovariectomized Rats with Alzheimer’s

by
Parisa Habibi
1,2,
Siamak Shahidi
1,*,
Maryam Khajvand-Abedini
3,
Zahra Shahabi
1,
Nasser Ahmadiasl
4,
Mohammad Reza Alipour
5,
Mahdi Ramezani
6 and
Alireza Komaki
1
1
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan 651783873, Iran
2
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran 1461884513, Iran
3
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan 651783873, Iran
4
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz 5166616471, Iran
5
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz 5166616471, Iran
6
Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan 651783873, Iran
*
Author to whom correspondence should be addressed.
Brain Sci. 2024, 14(7), 656; https://doi.org/10.3390/brainsci14070656
Submission received: 5 May 2024 / Revised: 22 June 2024 / Accepted: 24 June 2024 / Published: 28 June 2024
(This article belongs to the Special Issue Animal Models of Neurological Disorders)
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Abstract

Menopause may increase the risk of Alzheimer’s disease (AD) dementia. This study aimed to use young plasma therapy (YPT) to improve dementia caused by AD in aged ovariectomized rats. Female Wistar rats were used in the following groups: (a) young (CY) (180–200 g, 2–3 months, n = 10) and (b) old groups (250–350 g, 22–24 months, n = 60). The old rats were randomly assigned to six sub-groups: (1) control, (2) sham, (3) ovariectomized group (OVX), (4) OVX + Alzheimer disease (OVX + AD), (5) OVX + AD+ 17β-Estradiol (OVX + AD + E), and (6) OVX + AD + young plasma (OVX + AD + YP). Cognitive behaviors were evaluated using NOR, MWM, and PAL tests. MiR-134a, SIRT-1, CREB, and BDNF expressions were measured using real-time PCR and western blot, respectively. Oxidative stress in hippocampal tissue was assayed using ELISA kits. OVX and AD caused significant cognitive impairment (p < 0.001), up-regulated miR-134a (p < 0.001), down-regulated SIRT-1, CREB, and BDNF protein expression (p < 0.001), and decreased antioxidant marker levels (p < 0.001) compared to the sham group. YPT significantly restored miR-134a (p < 0.001), SIRT-1 (p < 0.001), CREB (p < 0.001), and BDNF (p < 0.001) protein expression in OVX + AD rats. YPT, as much as or more than estrogen therapy (ERT), significantly improved oxidative stress and down-regulated miR-134a expression and the up-regulation of SIRT-1, CREB, and BDNF proteins in OVX + AD rats (p < 0.001). YPT significantly improved histological alteration compared to the OVX + AD group (p < 0.001). As a non-pharmacological treatment, YPT can improve the expression of miR-134a and SIRT-1, CREB, and BDNF proteins as much as or more than estrogen therapy, ameliorating AD-induced dementia in aged OVX rats.
Keywords: ovariectomy; Alzheimer; young plasma; estrogen; miR-134a ovariectomy; Alzheimer; young plasma; estrogen; miR-134a
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MDPI and ACS Style

Habibi, P.; Shahidi, S.; Khajvand-Abedini, M.; Shahabi, Z.; Ahmadiasl, N.; Alipour, M.R.; Ramezani, M.; Komaki, A. Effect of Young Plasma Therapy on Cognition, Oxidative Stress, miRNA-134, BDNF, CREB, and SIRT-1 Expressions and Neuronal Survey in the Hippocampus of Aged Ovariectomized Rats with Alzheimer’s. Brain Sci. 2024, 14, 656. https://doi.org/10.3390/brainsci14070656

AMA Style

Habibi P, Shahidi S, Khajvand-Abedini M, Shahabi Z, Ahmadiasl N, Alipour MR, Ramezani M, Komaki A. Effect of Young Plasma Therapy on Cognition, Oxidative Stress, miRNA-134, BDNF, CREB, and SIRT-1 Expressions and Neuronal Survey in the Hippocampus of Aged Ovariectomized Rats with Alzheimer’s. Brain Sciences. 2024; 14(7):656. https://doi.org/10.3390/brainsci14070656

Chicago/Turabian Style

Habibi, Parisa, Siamak Shahidi, Maryam Khajvand-Abedini, Zahra Shahabi, Nasser Ahmadiasl, Mohammad Reza Alipour, Mahdi Ramezani, and Alireza Komaki. 2024. "Effect of Young Plasma Therapy on Cognition, Oxidative Stress, miRNA-134, BDNF, CREB, and SIRT-1 Expressions and Neuronal Survey in the Hippocampus of Aged Ovariectomized Rats with Alzheimer’s" Brain Sciences 14, no. 7: 656. https://doi.org/10.3390/brainsci14070656

APA Style

Habibi, P., Shahidi, S., Khajvand-Abedini, M., Shahabi, Z., Ahmadiasl, N., Alipour, M. R., Ramezani, M., & Komaki, A. (2024). Effect of Young Plasma Therapy on Cognition, Oxidative Stress, miRNA-134, BDNF, CREB, and SIRT-1 Expressions and Neuronal Survey in the Hippocampus of Aged Ovariectomized Rats with Alzheimer’s. Brain Sciences, 14(7), 656. https://doi.org/10.3390/brainsci14070656

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