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Amygdala Low-Frequency Stimulation Reduces Pathological Phase-Amplitude Coupling in the Pilocarpine Model of Epilepsy

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Department of Physiology, Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, 540142 Târgu Mureș, Romania
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Department of Pharmacology and Clinical Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureș, 540142 Târgu Mureș, Romania
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Author to whom correspondence should be addressed.
Brain Sci. 2020, 10(11), 856; https://doi.org/10.3390/brainsci10110856
Received: 15 October 2020 / Revised: 31 October 2020 / Accepted: 11 November 2020 / Published: 13 November 2020
Temporal-lobe epilepsy (TLE) is the most common type of drug-resistant epilepsy and warrants the development of new therapies, such as deep-brain stimulation (DBS). DBS was applied to different brain regions for patients with epilepsy; however, the mechanisms of action are not fully understood. Therefore, we tried to characterize the effect of amygdala DBS on hippocampal electrical activity in the lithium-pilocarpine model in male Wistar rats. After status epilepticus (SE) induction, seizure patterns were determined based on continuous video recordings. Recording electrodes were inserted in the left and right hippocampus and a stimulating electrode in the left basolateral amygdala of both Pilo and age-matched control rats 10 weeks after SE. Daily stimulation protocol consisted of 4 × 50 s stimulation trains (4-Hz, regular interpulse interval) for 10 days. The hippocampal electroencephalogram was analyzed offline: interictal epileptiform discharge (IED) frequency, spectral analysis, and phase-amplitude coupling (PAC) between delta band and higher frequencies were measured. We found that the seizure rate and duration decreased (by 23% and 26.5%) and the decrease in seizure rate correlated negatively with the IED frequency. PAC was elevated in epileptic animals and DBS reduced the pathologically increased PAC and increased the average theta power (25.9% ± 1.1 vs. 30.3% ± 1.1; p < 0.01). Increasing theta power and reducing the PAC could be two possible mechanisms by which DBS may exhibit its antiepileptic effect in TLE; moreover, they could be used to monitor effectiveness of stimulation. View Full-Text
Keywords: temporal lobe epilepsy; pilocarpine; amygdala; deep brain stimulation; hippocampus; phase-amplitude coupling; interictal discharges temporal lobe epilepsy; pilocarpine; amygdala; deep brain stimulation; hippocampus; phase-amplitude coupling; interictal discharges
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MDPI and ACS Style

Mihály, I.; Orbán-Kis, K.; Gáll, Z.; Berki, Á.-J.; Bod, R.-B.; Szilágyi, T. Amygdala Low-Frequency Stimulation Reduces Pathological Phase-Amplitude Coupling in the Pilocarpine Model of Epilepsy. Brain Sci. 2020, 10, 856. https://doi.org/10.3390/brainsci10110856

AMA Style

Mihály I, Orbán-Kis K, Gáll Z, Berki Á-J, Bod R-B, Szilágyi T. Amygdala Low-Frequency Stimulation Reduces Pathological Phase-Amplitude Coupling in the Pilocarpine Model of Epilepsy. Brain Sciences. 2020; 10(11):856. https://doi.org/10.3390/brainsci10110856

Chicago/Turabian Style

Mihály, István, Károly Orbán-Kis, Zsolt Gáll, Ádám-József Berki, Réka-Barbara Bod, and Tibor Szilágyi. 2020. "Amygdala Low-Frequency Stimulation Reduces Pathological Phase-Amplitude Coupling in the Pilocarpine Model of Epilepsy" Brain Sciences 10, no. 11: 856. https://doi.org/10.3390/brainsci10110856

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