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Article

Towards Bimodal Optical Monitoring of Photodynamic Therapy with Targeted Nanoconstructs: A Phantom Study

1
Institute of Applied Physics of the Russian Academy of Science, 603950 Nizhny Novgorod, Russia
2
Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
3
Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA
*
Author to whom correspondence should be addressed.
Appl. Sci. 2019, 9(9), 1918; https://doi.org/10.3390/app9091918
Received: 4 April 2019 / Revised: 5 May 2019 / Accepted: 7 May 2019 / Published: 10 May 2019
Increase of the efficiency of photodynamic therapy (PDT) requires the development of advanced protocols employing both novel photosensitizer (PS) carriers and aids for online monitoring. Nanoconstructs may be comprised of a photosensitizer, chemotherapy drugs, or inhibitors of molecular pathways that support cancer growth. In this paper, we analyze the efficiency of a bimodal approach involving fluorescence and optoacoustic imaging in monitoring drug distribution and photobleaching. The study evaluates typical sensitivities of these techniques to the presence of the two key moieties of a nanoconstruct: benzoporphyrin derivatives (BPD) serving as a PS, and IRDye800 acting as a contrast agent. Both imaging modalities employ dual-wavelength probing at the wavelengths corresponding to absorption peaks of BPD and IRDye800, which enables their separate detection. In an experiment on a tissue-mimicking phantom with inclusions containing separate BPD and IRDye800 solutions, fluorescence imaging demonstrated higher contrast as compared to optoacoustic imaging for both components, though strong light scattering in the surrounding media restricted accurate localization of the markers. It was also sensitive to photobleaching, which is a measure of PDT efficiency. Optoacoustic imaging demonstrated sufficient sensitivity to both components, though less than that of fluorescence imaging, however, it enabled depth-resolved detection of an absorber and estimation of its relative content. Employment of the bimodal approach in monitoring of PS photobleaching adds to its potential in intraprocedural PDT monitoring. View Full-Text
Keywords: photodynamic therapy; photosensitizer; optoacoustic imaging; fluorescence imaging photodynamic therapy; photosensitizer; optoacoustic imaging; fluorescence imaging
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MDPI and ACS Style

Kurakina, D.; Kirillin, M.; Perekatova, V.; Plekhanov, V.; Orlova, A.; Sergeeva, E.; Khilov, A.; Nerush, A.; Subochev, P.; Mallidi, S.; Turchin, I.; Hasan, T. Towards Bimodal Optical Monitoring of Photodynamic Therapy with Targeted Nanoconstructs: A Phantom Study. Appl. Sci. 2019, 9, 1918. https://doi.org/10.3390/app9091918

AMA Style

Kurakina D, Kirillin M, Perekatova V, Plekhanov V, Orlova A, Sergeeva E, Khilov A, Nerush A, Subochev P, Mallidi S, Turchin I, Hasan T. Towards Bimodal Optical Monitoring of Photodynamic Therapy with Targeted Nanoconstructs: A Phantom Study. Applied Sciences. 2019; 9(9):1918. https://doi.org/10.3390/app9091918

Chicago/Turabian Style

Kurakina, Daria, Mikhail Kirillin, Valeriya Perekatova, Vladimir Plekhanov, Anna Orlova, Ekaterina Sergeeva, Aleksandr Khilov, Anastasiya Nerush, Pavel Subochev, Srivalleesha Mallidi, Ilya Turchin, and Tayyaba Hasan. 2019. "Towards Bimodal Optical Monitoring of Photodynamic Therapy with Targeted Nanoconstructs: A Phantom Study" Applied Sciences 9, no. 9: 1918. https://doi.org/10.3390/app9091918

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