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Special Issue on the Environmental Fate of Emerging Organic Micro-Contaminants
Open AccessArticle

Distinct Bacterial Consortia Established in ETBE-Degrading Enrichments from a Polluted Aquifer

1
Université Lyon1, CNRS, UMR5557, Ecologie Microbienne, INRA, 69622 Villeurbanne CEDEX, France
2
Biology Centre of the Czech Academy of Sciences, Institute of Soil Biology, 37005 České Budějovice, Czech Republic
3
University of Groningen, Groningen Institute for Evolutionary Life Sciences, 9747 AG Groningen, The Netherlands
4
SERPOL, 69200 Vénissieux, France
5
IFP Energies Nouvelles, 92852 Rueil-Malmaison, France
*
Author to whom correspondence should be addressed.
Appl. Sci. 2019, 9(20), 4247; https://doi.org/10.3390/app9204247
Received: 16 August 2019 / Revised: 27 September 2019 / Accepted: 2 October 2019 / Published: 11 October 2019
(This article belongs to the Special Issue Environmental Fate of Emerging Organic Micro-Contaminants)
Ethyl tert-butyl ether (ETBE) is a gasoline additive that became an important aquifer pollutant. The information about natural bacterial consortia with a capacity for complete ETBE degradation is limited. Here we assess the taxonomical composition of bacterial communities and diversity of the ethB gene (involved in ETBE biodegradation) in ETBE-enrichment cultures that were established from a gasoline-polluted aquifer, either from anoxic ETBE-polluted plume water (PW), or from an upstream non-polluted water (UW). We used a 16S rRNA microarray, and 16S rRNA and ethB gene sequencing. Despite the dissimilar initial chemical conditions and microbial composition, ETBE-degrading consortia were obtained from both PW and UW. The composition of ETBE-enrichment cultures was distinct from their initial water samples, reflecting the importance of the rare biosphere as a reservoir of potential ETBE degraders. No convergence was observed between the enrichment cultures originating from UW and PW, which were dominated by Mesorhizobium and Hydrogenophaga, respectively, indicating that distinct consortia with the same functional properties may be present at one site. Conserved ethB genes were evidenced in both PW and UW ETBE-enrichment cultures and in PW water. Our results suggest that the presence of ethB genes rather than the taxonomical composition of in situ bacterial communities indicate the potential for the ETBE degradation at a given site. View Full-Text
Keywords: ethyl tert-butyl ether; ETBE biodegradation; bacterial community; polluted aquifer; fuel oxygenates; ethB gene ethyl tert-butyl ether; ETBE biodegradation; bacterial community; polluted aquifer; fuel oxygenates; ethB gene
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Kyselková, M.; Falcão Salles, J.; Dumestre, A.; Benoit, Y.; Grundmann, G.L. Distinct Bacterial Consortia Established in ETBE-Degrading Enrichments from a Polluted Aquifer. Appl. Sci. 2019, 9, 4247.

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