Inoculation with Glomus mosseae Improves the Growth and Salvianolic Acid B Accumulation of Continuously Cropped Salvia miltiorrhiza
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Mycorrhizal Inocula
2.3. Plant Material
2.4. Root Staining for Evaluation of AMF Root Colonization
2.5. Assessment of Plant Disease and Growth
2.6. Plant Nutrient Analysis
2.7. Quantitative Analysis of Phenolic Acids
2.8. Statistical Analysis
3. Results
3.1. AMF Root Colonization
3.2. Effects of AMF on Field Diseases and S. miltiorrhiza Growth
3.3. Effect of G. mosseae on Phenolic Acids in S. miltiorrhiza
3.4. Effect of AMF on Plant Mineral Nutrient Status
4. Discussion
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Treatments | Biomass of Shoot (g) | Biomass of Root (g) | Leaf Number | Crown Width (cm) | Plant Height (cm) | Stem Diameter (cm) | Radical Number | Root | ||
---|---|---|---|---|---|---|---|---|---|---|
Length (cm) | Diameter (cm) | Number | ||||||||
CK | 36.44 ± 1.70 b | 34.11 ± 3.19 b | 74.74 ± 11.99 a | 38.56 ± 1.62 b | 39.58 ± 2.67 b | 1.97 ± 0.09 b | 4.31 ± 1.89 a | 30.60 ± 0.79 b | 1.82 ± 0.16 a | 31.48 ± 2.47 a |
GM | 53.97 ± 3.87 a | 47.47 ± 2.87 a | 84.75 ± 4.14 a | 42.66 ± 1.04 a | 49.86 ± 1.72 a | 2.33 ± 0.08 a | 7.70 ± .93 a | 36.64 ± 2.23 a | 1.99 ± 0.19 a | 30.82 ± 0.43 a |
Treatments | Rosmarinic Acid (%) | Salvianolic Acid B (%) | |
---|---|---|---|
Shoot | CK | 0.89 ± 0.02 a | 1.14 ± 0.02 b |
GM | 0.63 ± 0.09 b | 1.39 ± 0.09 a | |
Root | CK | 0.33 ± 0.03 a | 0.76 ± 0.00 b |
GM | 0.34 ± 0.02 a | 0.83 ± 0.01 a |
Treatments | N (g/kg) | P (g/kg) | K (g/kg) | Ca (g/kg) | Mg (g/kg) | Cu (mg/kg) | Zn (mg/kg) | Fe (g/kg) | Mn (mg/kg) | |
---|---|---|---|---|---|---|---|---|---|---|
Shoot | CK | 16.40 ± 0.75 a | 4.31 ± 0.28 a | 26.08 ± 0.91 b | 2.37 ± 0.05 a | 5.03 ± 0.12 a | 16.41 ± 1.11 a | 75.03 ± 6.68 a | 1.48 ± 0.12 a | 65.93 ± 1.07 c |
GM | 18.26 ± 1.07 a | 3.24 ± 0.11 b | 32.41 ± 0.24 a | 2.48 ± 0.06 a | 4.29 ± 0.24 a | 17.87 ± 0.38 a | 74.28 ± 3.04 a | 1.20 ± 0.12 b | 127.49 ± 10.2 a | |
Root | CK | 15.21 ± 0.94 a | 2.87 ± 0.03 a | 10.35 ± 0.11 a | 0.53 ± 0.03 a | 2.67 ± 0.02 a | 18.01 ± 0.29 a | 44.01 ± 2.49 a | 0.76 ± 0.03 b | 33.8 ± 1.33 b |
GM | 13.88 ± 0.67 a | 2.51 ± 0.04 b | 10.07 ± 0.26 a | 0.54 ± 0.01 a | 2.45 ± 0.07 b | 21.03 ± 1.80 a | 42.97 ± 2.40 a | 1.33 ± 0.11 a | 55.82 ± 5.94 a |
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Chen, M.; Yang, G.; Liu, D.; Li, M.; Qiu, H.; Guo, L.; Huang, L.; Chao, Z. Inoculation with Glomus mosseae Improves the Growth and Salvianolic Acid B Accumulation of Continuously Cropped Salvia miltiorrhiza. Appl. Sci. 2017, 7, 692. https://doi.org/10.3390/app7070692
Chen M, Yang G, Liu D, Li M, Qiu H, Guo L, Huang L, Chao Z. Inoculation with Glomus mosseae Improves the Growth and Salvianolic Acid B Accumulation of Continuously Cropped Salvia miltiorrhiza. Applied Sciences. 2017; 7(7):692. https://doi.org/10.3390/app7070692
Chicago/Turabian StyleChen, Meilan, Guang Yang, Dahui Liu, Minhui Li, Hongyan Qiu, Lanping Guo, Luqi Huang, and Zhi Chao. 2017. "Inoculation with Glomus mosseae Improves the Growth and Salvianolic Acid B Accumulation of Continuously Cropped Salvia miltiorrhiza" Applied Sciences 7, no. 7: 692. https://doi.org/10.3390/app7070692