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Appl. Sci. 2016, 6(9), 246;

Distinguishing Bovine Fecal Matter on Spinach Leaves Using Field Spectroscopy

School of Biosystems and Food Engineering, University College Dublin, Dublin 4, Ireland
Environmental Microbial and Food Safety Laboratory, US Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, Maryland, MD 20705, USA
Author to whom correspondence should be addressed.
Academic Editor: Kuanglin Kevin Chao
Received: 6 May 2016 / Revised: 29 July 2016 / Accepted: 23 August 2016 / Published: 30 August 2016
(This article belongs to the Special Issue Applications of Hyperspectral Imaging for Food and Agriculture)
Full-Text   |   PDF [1318 KB, uploaded 30 August 2016]   |  


Detection of fecal contaminants on leafy greens in the field will allow for decreasing cross-contamination of produce during and post-harvest. Fecal contamination of leafy greens has been associated with Escherichia coli (E. coli) O157:H7 outbreaks and foodborne illnesses. In this study, passive field spectroscopy measuring reflectance and fluorescence created by the sun’s light, coupled with numerical normalization techniques, are used to distinguish fecal contaminants on spinach leaves from soil on spinach leaves and uncontaminated spinach leaf portions. A Savitzky-Golay first derivative transformation and a waveband ratio of 710:688 nm as normalizing techniques were assessed. A soft independent modelling of class analogies (SIMCA) procedure with a 216 sample training set successfully predicted all 54 test set sample types using the spectral region of 600–800 nm. The ratio of 710:688 nm along with set thresholds separated all 270 samples by type. Application of these techniques in-field to avoid harvesting of fecal contaminated leafy greens may lead to a reduction in foodborne illnesses as well as reduced produce waste. View Full-Text
Keywords: fecal contaminants; leafy greens; in-field; field spectroscopy fecal contaminants; leafy greens; in-field; field spectroscopy

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Everard, C.D.; Kim, M.S.; O’Donnell, C.P. Distinguishing Bovine Fecal Matter on Spinach Leaves Using Field Spectroscopy. Appl. Sci. 2016, 6, 246.

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