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Appl. Sci. 2016, 6(5), 139;

Single Cell Chemotactic Responses of Helicobacter pylori to Urea in a Microfluidic Chip

BioNano Laboratory, School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada
Author to whom correspondence should be addressed.
Academic Editor: Donglei (Emma) Fan
Received: 23 February 2016 / Revised: 6 April 2016 / Accepted: 25 April 2016 / Published: 7 May 2016
(This article belongs to the Special Issue Nanomanufacturing of Biomedical Systems and Devices)
Full-Text   |   PDF [908 KB, uploaded 7 May 2016]   |  


Helicobacter pylori is a spiral-shaped bacterium that grows in the human digestive tract; it infects ~50% of the global population. H. pylori induce inflammation, gastroenteritis, and ulcers, which is associated with significant morbidity and may be linked to stomach cancer in certain individuals. Motility is an essential virulence factor for H. pylori, allowing it to migrate toward and invade the epithelial lining of the stomach to shelter it from the harsh environment of the stomach. H. pylori senses pH gradients and use polar flagella to move towards the epithelium where the pH approaches neutrality. However, its chemotaxis behaviors are incompletely understood. Previous in vitro tests examining the response of H. pylori to chemical gradients have been subjected to substantial limitations. To more accurately mimic/modulate the cellular microenvironment, a nanoporous microfluidic device was used to monitor the real time chemotactic activity of single cell of H. pylori in response to urea. The results showed that microfluidic method is a promising alternative for accurate studying of chemotactic behavior of H. pylori, the application of which may also be extended in the studies of other bacteria. View Full-Text
Keywords: Helicobacter pylori; chemotaxis; microfluidics; urea; cell motility Helicobacter pylori; chemotaxis; microfluidics; urea; cell motility

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Weng, X.; Neethirajan, S.; Vogt, A. Single Cell Chemotactic Responses of Helicobacter pylori to Urea in a Microfluidic Chip. Appl. Sci. 2016, 6, 139.

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