Bioremediation of Crude Oil by Corynebacterium stationis CsPe-1: A Preliminary In Vitro Study
, Nélida Milly Otiniano 1, Walter Rojas-Villacorta 2, Segundo Rojas-Flores 1, Luis Cabanillas-Chirinos 1 and Michel Abanto Marin 3
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsComments attached
Comments for author File: 
Comments.pdf
I don't feel qualified to give feedback Quality of English Language
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsThe manuscript "Bioremediation of Crude Oil by Corynebacterium stationis: A preliminary in vitro study" describes the role of the bacterial strain in the degradation of crude oil under laboratory conditions. Although the study does not bring any major innovations in the field of bioremediation, the study is solid, the results are clear and adequately supported by statistical analyses. The text is well written, fluid and presents the results and discussion in an objective and clear manner. In Figure 1, the Y bar should be graduated up to 100%, this is a rule when dealing with percentages in statistics. In Figure 2 the names of the microorganisms should be written in italics.
You should bring a figure with an overview of the sequenced genome; it is customary to present the results that were proposed in material and methods. I suggest at least an overview of the bacterial chromosome next to a close strain.
Author Response
"Please see the attachment."
Author Response File: Author Response.pdf
Reviewer 3 Report
Comments and Suggestions for AuthorsThe manuscript " Bioremediation of Crude Oil by Corynebacterium stationis: A preliminary in vitro study" presents information on potential biodegradation on petroleum organic pollutants by C. stationis. The topic of the study is interesting and important. The topic of bioremediation of both soil and water environments is very important. New microorganisms with biodegradation potential of xenobiotics, including oil, are constantly being sought and described.
General comments:
Title: the strain number should be given in the title
The introduction lacks information on the scale of the problem, why is the topic important? In the introduction, the Authors write about the biodegradation of oil by specific microorganisms e.g. bacteria and fungi. But there are no information about enzymes they synthesize that participate in the enzymatic biodegradation of these compounds. Moreover, please provide information on the biodegradation potential of C. stationis bacteria, also in relation to other xenobiotics. This will emphasize the importance of this bacterium in environmental bioremediation.
If the strain C. stationis was from a laboratory of the Instituto y Centro de Investigación de la Universidad Cesar Vallejo (Trujillo, Peru) had it not been previously genetically identified? Please explain
Minor issues:
Line 36: please dilate cited literature repetition
Line 133: in System 1 inoculum appears twice: “10% inoculum” and again “50 ml of C. stationis” inoculum please explain
Line 226: The Author wrote that bacteria probably biodegradate using of enzymes
(oxydenases, peroxidases):, so it is probably an enzymatic transformation. Are there any specific enzymes known that participate in this process? and the products that are formed during enzymatic transformation of oil?
Author Response
"Please see the attachment."
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Comments and Suggestions for AuthorsAll observations were made and now the manuscript is of better quality.
