DNA Barcoding and Molecular Phylogenetics Revealed a New Cryptic Bamboo Aphid Species of the Genus Takecallis (Hemiptera: Aphididae)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Specimen Collection and Identification
2.2. DNA Sequencing and Phylogenetic Analysis
2.3. Species Delimitation
3. Results
3.1. Molecular Phylogeny
3.2. Species Delimitation
3.3. Taxonomy
3.3.1. Differential Diagnosis
3.3.2. Species Description
3.3.3. Key to Species of the Genus Takecallis
- Antennae longer than body..................................................................................2
- –
- Antennae shorter than body.............................................................................8
- Ultimate rostral segment without any accessory setae. Abdominal tergites 1–7 with paired dark circular spots around spinal setae...............................................T. assumenta
- –
- Ultimate rostral segment with 3–4 accessory setae. Abdominal tergites 1–7 with or without dark spots....................................................................................3
- Antennal segment I with (4-) 5–7 setae. Antennal segments III–VI dark, except for basal part of antennal segment III. Antennal segment III with 4–15 secondary rhinaria................................................................................................4
- –
- Antennal segment I usually with 4, rarely with 3 or 5, setae. Antennal segments III–VI or IV–VI base usually pale basally and dark apically. Antennal segment III with 3–9 secondary rhinaria.....................................................................................6
- Antennal segment III with 10–15 secondary rhinaria, marginal seta on abdominal tergite VI not positioned on base of siphunculus.................................................T. affinis s. str.
- –
- Antennal segment III with 4–7 (often 5) secondary rhinaria, marginal seta on abdominal tergite VI positioned on base of siphunculus............................................5
- Antennae 1.3 times body length, antennal segment I with 5–7 setae, cauda with 18 setae, each lobe of anal plate with 15 setae, spinal abdominal setae on low elevations..........................................................T. affinis ssp. niitakayamensis
- –
- Antennae 0.95–1.14 times body length, antennal segment I with 4 setae, cauda with 8–11 setae, each lobe of anal plate with 5–9 setae, spinal abdominal setae barely visible................................................................ T. nigroantennatus sp. nov.
- Thorax and abdomen usually without dark dorsal markings, never with paired elongate dark abdominal patches. Cauda slightly dusky or blackish...............................................7
- –
- Thorax with variably-developed longitudinal dark stripes, and abdomen with a pair of elongate dark patches on each tergite. Cauda pale or dusky....................T. arundinariae
- Antennae 3.36–4.00 mm, secondary rhinaria densely concentrated on very short dark
- –
- Section of proximal third of antennal segment III...........................................T. alba
- –
- Antennae 2.36–2.51 mm, secondary rhinaria spread over longer dark section oc cupying most of proximal third of antennal segment III...........................................T. arundicolens
- Secondary rhinaria confined to basal third of antennal segment III. Each abdominal tergite bearing only 2 spinal setae .................................................................T. taiwana
- –
- Secondary rhinaria extending about half of length of antennal segment III. Each abdominal tergite bearing at least 4 setae besides the marginal ones...................T. sasae
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample ID | COI | EF1 | Reference | |
---|---|---|---|---|
Takecallis nigroantennatus sp. nov—isolate 1BAA | OR001758 | Present Study | OR039806 | Present Study |
Takecallis nigroantennatus sp. nov—isolate 2BAN | OR001759 | Present Study | OR039807 | Present Study |
Takecallis nigroantennatus sp. nov—isolate 3BAN | OR001760 | Present Study | OR039808 | Present Study |
Takecallis nigroantennatus sp. nov—isolate 4BAA | OR001761 | Present Study | OR039809 | Present Study |
Takecallis nigroantennatus sp. nov—isolate 5BAN | OR001762 | Present Study | OR039810 | Present Study |
Takecallis nigroantennatus sp. nov—isolate 6BAA | OR001763 | Present Study | OR039811 | Present Study |
Takecallis nigroantennatus sp. nov—isolate 7BAN | OR001764 | Present Study | OR039812 | Present Study |
Takecallis taiwana | MH820965 | [30] | MT039369 | Direct submission |
MH820989 | [30] | |||
MH820958 | [30] | |||
MH820978 | [30] | |||
MH820985 | [30] | |||
MH820961 | [30] | |||
MH820975 | [30] | |||
MH820966 | [30] | |||
MH820962 | [30] | |||
MH820968 | [30] | |||
MH820980 | [30] | |||
MH820981 | [30] | |||
MH820996 | [30] | |||
MH820990 | [30] | |||
MH820973 | [30] | |||
MH820993 | [30] | |||
MH820974 | [30] | |||
MH820977 | [30] | |||
MH820970 | [30] | |||
MH820987 | [30] | |||
MH820964 | [30] | |||
MH820988 | [30] | |||
MH820979 | [30] | |||
MH820963 | [30] | |||
MH820984 | [30] | |||
MH820991 | [30] | |||
MH820967 | [30] | |||
MH820982 | [30] | |||
MH820976 | [30] | |||
MH820997 | [30] | |||
MH820992 | [30] | |||
MH820969 | [30] | |||
MH820995 | [30] | |||
MH820959 | [30] | |||
MH820951 | [30] | |||
Takecallis sasae | KY307057 | [6] | MT262445 | Direct submission |
KY307060 | [6] | |||
KY307059 | [6] | |||
KY307063 | [6] | |||
KY307061 | [6] | |||
KY307062 | [6] | |||
KY307058 | [6] | |||
KY307056 | [6] | |||
Takecallis aroundicolens | EU701915 | [1] | MT262446 | Direct submission |
KY307036 | [6] | |||
KY307039 | [6] | |||
KY307037 | [6] | |||
KY307038 | [6] | |||
KY307035 | [6] | |||
KY307047 | [6] | |||
KY307049 | [6] | |||
KY307051 | [6] | |||
KY307050 | [6] | |||
KY307034 | [6] | |||
KY307048 | [6] | |||
KY307029 | [6] | |||
KY307030 | [6] | |||
KY307043 | [6] | |||
KY307031 | [6] | |||
KY307053 | [6] | |||
KY307032 | [6] | |||
KY307028 | [6] | |||
KY307033 | [6] | |||
KY307046 | [6] | |||
KY307052 | [6] | |||
KY307045 | [6] | |||
KY307044 | [6] | |||
KY307040 | [6] | |||
KY307041 | [6] | |||
KY307042 | [6] | |||
KY307027 | [6] | |||
KY307022 | [6] | |||
KY307024 | [6] | |||
KY307025 | [6] | |||
KY307023 | [6] | |||
KY307026 | [6] | |||
KY307049 | [6] | |||
Takecallis arundinariae | KR039454 | [31] | MT262444 | Direct submission |
MH820948 | [30] | |||
MH820950 | [30] | |||
MH820955 | [30] | |||
MH820956 | [30] | |||
MH820947 | [30] | |||
MH820954 | [30] | |||
MH820957 | [30] | |||
MH820952 | [30] | |||
KR579007 | [32] | |||
MH820951 | [30] | |||
JX205217 | Direct submission | |||
KR034713 | [31] | |||
MH820953 | [30] | |||
KR034488 | [31] | |||
KY307054 | [6] | |||
KF639650 | Direct submission | |||
KY307055 | [6] | |||
KR043148 | [31] | |||
GU135641 | [33] | |||
Euceraphis lineata | KR033551 | [31] | ||
Calaphis magnoliae | KY306818 | [6] | MT262369 | [9] |
Glyphinaphis bambusae | - | MN167448 | Direct submission |
No. | Body | Antenna | Antennal Segments | Ultimate Rostral Segment | Second Segment of Hind Tarsus | |||
---|---|---|---|---|---|---|---|---|
III | IV | V | VIbase + VI Processus Terminalis | |||||
1 Holotype | 1.79 | 1.88 | 0.64 | 0.39 | 0.34 | 0.21 + 0.20 | 0.07 | 0.10 |
2 | 1.42 | 1.62 | 0.52 | 0.35 | 0.30 | 0.19 + 0.20 | 0.07 | 0.11 |
3 | 1.40 | 1.57 | 0.47 | 0.35 | 0.29 | 0.19 + 0.19 | 0.07 | 0.12 |
4 | 1.75 | 1.89 | 0.59 | 0.41 | 0.35 | 0.22 + 0.21 | 0.08 | 0.12 |
5 | 1.74 | 1.87 | 0.64 | 0.45 | 0.40 | 0.21 + 0.22 | 0.07 | 0.10 |
6 | 1.70 | 1.87 | 0.62 | 0.39 | 0.33 | 0.20 + 0.21 | 0.08 | 0.10 |
7 | 1.74 | 1.85 | 0.63 | 0.56 | 0.33 | 0.22 + 0.21 | 0.08 | 0.11 |
8 | 1.95 | 1.87 | 0.64 | 0.45 | 0.38 | 0.22 + 0.22 | 0.07 | 0.10 |
9 | 1.74 | 1.90 | 0.63 | 0.43 | 0.36 | 0.25 + 0.20 | 0.07 | 0.09 |
10 | 1.86 | 1.89 | 0.63 | 0.48 | 0.33 | 0.20 + 0.21 | 0.08 | 0.11 |
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Wieczorek, K.; Sawka-Gądek, N. DNA Barcoding and Molecular Phylogenetics Revealed a New Cryptic Bamboo Aphid Species of the Genus Takecallis (Hemiptera: Aphididae). Appl. Sci. 2023, 13, 7798. https://doi.org/10.3390/app13137798
Wieczorek K, Sawka-Gądek N. DNA Barcoding and Molecular Phylogenetics Revealed a New Cryptic Bamboo Aphid Species of the Genus Takecallis (Hemiptera: Aphididae). Applied Sciences. 2023; 13(13):7798. https://doi.org/10.3390/app13137798
Chicago/Turabian StyleWieczorek, Karina, and Natalia Sawka-Gądek. 2023. "DNA Barcoding and Molecular Phylogenetics Revealed a New Cryptic Bamboo Aphid Species of the Genus Takecallis (Hemiptera: Aphididae)" Applied Sciences 13, no. 13: 7798. https://doi.org/10.3390/app13137798