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Open AccessArticle

Influence of Ozone on the Biochemical Composition of Birch Sap

1
Institute of Energy and Biotechnology Engineering, Agriculture Academy, Vytautas Magnus University, K. Donelaičio str. 58, 44248 Kaunas, Lithuania
2
Institute of Forest Biology and Silviculture, Agriculture Academy, Vytautas Magnus University, K. Donelaičio str. 58, 44248 Kaunas, Lithuania
3
Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kaunas str. 30, LT-54333 Babtai, Kaunas District, Lithuania
*
Author to whom correspondence should be addressed.
Academic Editor: Claudio Medana
Appl. Sci. 2021, 11(7), 2965; https://doi.org/10.3390/app11072965
Received: 4 March 2021 / Revised: 23 March 2021 / Accepted: 24 March 2021 / Published: 26 March 2021
(This article belongs to the Special Issue Biochemical Composition of Food)
Studies have shown that ozone is a good oxidizer and a strong disinfectant. There are many uses for ozone in the food industry, but there is relatively little information about the influence of ozone on biochemical composition and the capacity to reduce the number of microorganisms in birch sap. In this study, sap was ozonated at different intervals for 5 min (O3: 0.087 ± 0.009 mg L−1), 10 min, 15 min, 20 min, 25 min, or 30 min (O3: 0.99 ± 0.09 mg L−1). The parameters of the birch sap were studied immediately after the ozone treatment as well as during storage for seven days at 2 °C and for five days at 20 °C. The parameters of ozonated birch sap were compared with the parameters of fresh sap (control). The microbiological analysis included total bacterial count, lactic acid bacterial count, and yeast and mold count. Birch sap color, pH, titratable acidity, and ºBrix values were also determined. Evaluation of monosaccharides, sucrose, total sugars, and ascorbic acid was carried out in fresh sap as well as sap ozonated for 30 min, immediately after ozonation. The results show the statistical significance of the inactivation of microorganisms after treatment in most cases. The microorganism counts gradually reduced with increasing intervals of ozone treatment. The best results were obtained after 25 and 30 min of ozonation. Ozone treatment did not significantly influence the pH, titratable acidity, or °Brix statistically. Values of monosaccharides, sucrose, total sugars, and ascorbic acid were influenced within the margin of error. Ozone had a significant influence on the chroma and hue angle. View Full-Text
Keywords: Betula pendula; biochemical composition; birch sap; ozone treatment; microbiological contamination; quality Betula pendula; biochemical composition; birch sap; ozone treatment; microbiological contamination; quality
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MDPI and ACS Style

Paulikienė, S.; Mingaila, J.; Vilimas, V.; Bartkevičius, E.; Viskelis, P.; Raila, A. Influence of Ozone on the Biochemical Composition of Birch Sap. Appl. Sci. 2021, 11, 2965. https://doi.org/10.3390/app11072965

AMA Style

Paulikienė S, Mingaila J, Vilimas V, Bartkevičius E, Viskelis P, Raila A. Influence of Ozone on the Biochemical Composition of Birch Sap. Applied Sciences. 2021; 11(7):2965. https://doi.org/10.3390/app11072965

Chicago/Turabian Style

Paulikienė, Simona; Mingaila, Justas; Vilimas, Vladas; Bartkevičius, Edmundas; Viskelis, Pranas; Raila, Algirdas. 2021. "Influence of Ozone on the Biochemical Composition of Birch Sap" Appl. Sci. 11, no. 7: 2965. https://doi.org/10.3390/app11072965

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