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Article

Fructans with Varying Degree of Polymerization Enhance the Selective Growth of Bifidobacterium animalis subsp. lactis BB-12 in the Human Gut Microbiome In Vitro

1
ProDigest BV, Technologiepark 82, 9052 Ghent, Belgium
2
PepsiCo R&D, 555 W Monroe St., Chicago, IL 60661, USA
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PepsiCo R&D, Leningradsky Prospect 72 k 4, 125319 Moscow, Russia
4
Center of Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
Appl. Sci. 2021, 11(2), 598; https://doi.org/10.3390/app11020598
Received: 13 December 2020 / Revised: 29 December 2020 / Accepted: 5 January 2021 / Published: 9 January 2021
(This article belongs to the Special Issue Functional Food and Nutrition)
Synbiotics aim to improve gastrointestinal health by combining pre- and probiotics. This study evaluated combinations of Bifidobacterium animalis subsp. lactis BB-12 with seven fructans: oligofructoses (OF1-OF2; low degree of polymerization (DP)), inulins (IN1-IN2-IN3; high DP) and OF/IN mixtures (OF/IN1-OF/IN2). During monoculture incubations, all fructans were fermented by BB-12 as followed from increased BB-12 numbers and increased acetate and lactate concentrations, with most pronounced fermentation for low DP fructans (OF1-OF2). Further, short-term colonic incubations for three human donors revealed that also in presence of a complex microbiota, all fructans (particularly OF1) consistently selectively enhanced the growth of BB-12. While each fructan as such already increased Bifidobacteriaceae numbers with 0.94–1.26 log(cells/mL), BB-12 co-supplementation additionally increased Bifidobacteriaceae with 0.17–0.46 log(cells/mL). Further, when co-supplemented with fructans, BB-12 decreased Enterobacteriaceae numbers (significant except for IN1-IN3). At metabolic level, all fructans decreased pH due to increased acetate and lactate production, while OF/IN2-IN1-IN2-IN3 also stimulated propionate and butyrate production. BB-12 co-supplementation further increased propionate and butyrate for OF/IN2-IN3 and IN1-IN2, respectively. Overall, combinations of BB-12 with fructans are promising synbiotic concepts, likely due to intracellular consumption of low DP-fructans by BB-12 (either present in starting product or released upon fermentation by indigenous microbes), thereby enhancing effects of the co-administered fructan. View Full-Text
Keywords: prebiotic; synbiotic; microbiota; colon; intestine; oligofructose; inulin; Bifidobacteria; Faecalibacterium; Blautia prebiotic; synbiotic; microbiota; colon; intestine; oligofructose; inulin; Bifidobacteria; Faecalibacterium; Blautia
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MDPI and ACS Style

Van den Abbeele, P.; Duysburgh, C.; Ghyselinck, J.; Goltz, S.; Berezhnaya, Y.; Boileau, T.; De Blaiser, A.; Marzorati, M. Fructans with Varying Degree of Polymerization Enhance the Selective Growth of Bifidobacterium animalis subsp. lactis BB-12 in the Human Gut Microbiome In Vitro. Appl. Sci. 2021, 11, 598. https://doi.org/10.3390/app11020598

AMA Style

Van den Abbeele P, Duysburgh C, Ghyselinck J, Goltz S, Berezhnaya Y, Boileau T, De Blaiser A, Marzorati M. Fructans with Varying Degree of Polymerization Enhance the Selective Growth of Bifidobacterium animalis subsp. lactis BB-12 in the Human Gut Microbiome In Vitro. Applied Sciences. 2021; 11(2):598. https://doi.org/10.3390/app11020598

Chicago/Turabian Style

Van den Abbeele, Pieter, Cindy Duysburgh, Jonas Ghyselinck, Shellen Goltz, Yulia Berezhnaya, Thomas Boileau, Anke De Blaiser, and Massimo Marzorati. 2021. "Fructans with Varying Degree of Polymerization Enhance the Selective Growth of Bifidobacterium animalis subsp. lactis BB-12 in the Human Gut Microbiome In Vitro" Applied Sciences 11, no. 2: 598. https://doi.org/10.3390/app11020598

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