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Open AccessArticle

Electronic Emulator of Biological Tissue as an Electrical Load during Electroporation

Faculty of Electrical Engineering, University of Ljubljana, Tržaska 25, SI-1000 Ljubljana, Slovenia
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Appl. Sci. 2020, 10(9), 3103; https://doi.org/10.3390/app10093103
Received: 17 March 2020 / Revised: 22 April 2020 / Accepted: 23 April 2020 / Published: 29 April 2020
(This article belongs to the Special Issue Electroporation Systems and Applications)
Electroporation is an emerging technology, with great potential in many different medical and biotechnological applications, food engineering and biomass processing. Large variations of biological load characteristics, however, represent a great challenge in electroporator design, which results in different solutions. Because a clinical electroporator is a medical device, it must comply with medical device regulative and standards. However, none of the existing standards directly address the operation or electroporator’s performance requirements. In order to evaluate clinical, laboratory and prototype electroporation devices during the development process, or to evaluate their final performance considering at least from the perspective of output pulse parameters, we present a case study on the design of an electronic emulator of biological tissue as an electrical load during electroporation. The proposed electronic load emulator is a proof of concept, which enables constant and sustainable testing and unbiased comparison of different electroporators’ operations. We developed an analog electrical circuit that has equivalent impedance to the beef liver tissue in combination with needle electrodes, during high voltage pulse delivery and/or electroporation. Current and voltage measurements during electroporation of beef liver tissue ex vivo, were analyzed and parametrized to define the analog circuit equation. An equivalent circuit was simulated, built and validated. The proposed concept of an electronic load emulator can be used for “classical” electroporator (i.e., not nanosecond) performance evaluation and comparison of their operation. Additionally, it facilitates standard implementation regarding the testing protocol and enables quality assurance. View Full-Text
Keywords: electroporation; tissue conductivity change; standard nonlinear load; biological load; electroporation emulator electroporation; tissue conductivity change; standard nonlinear load; biological load; electroporation emulator
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Pirc, E.; Balosetti, B.; Miklavčič, D.; Reberšek, M. Electronic Emulator of Biological Tissue as an Electrical Load during Electroporation. Appl. Sci. 2020, 10, 3103.

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