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AtTPR10 Containing Multiple ANK and TPR Domains Exhibits Chaperone Activity and Heat-Shock Dependent Structural Switching

Division of Applied Life Science (BK21+) and Plant Molecular Biotechnology Research Center, Gyeongsang National University, Jinju 52828, Korea
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Appl. Sci. 2020, 10(4), 1265; https://doi.org/10.3390/app10041265
Received: 13 January 2020 / Revised: 8 February 2020 / Accepted: 10 February 2020 / Published: 13 February 2020
Among the several tetratricopeptide (TPR) repeat-containing proteins encoded by the Arabidopsis thaliana genome, AtTPR10 exhibits an atypical structure with three TPR domain repeats at the C-terminus in addition to seven ankyrin (ANK) domain repeats at the N-terminus. However, the function of AtTPR10 remains elusive. Here, we investigated the biochemical function of AtTPR10. Bioinformatic analysis revealed that AtTPR10 expression is highly enhanced by heat shock compared with the other abiotic stresses, suggesting that AtTPR10 functions as a molecular chaperone to protect intracellular proteins from thermal stresses. Under the heat shock treatment, the chaperone activity of AtTPR10 increased significantly; this was accompanied by a structural switch from the low molecular weight (LMW) protein to a high molecular weight (HMW) complex. Analysis of two truncated fragments of AtTPR10 containing the TPR and ANK repeats showed that each domain exhibits a similar range of chaperone activity (approximately one-third of that of the native protein), suggesting that each domain cooperatively regulates the chaperone function of AtTPR10. Additionally, both truncated fragments of AtTPR10 underwent structural reconfiguration to form heat shock-dependent HMW complexes. Our results clearly demonstrate that AtTPR10 functions as a molecular chaperone in plants to protect intracellular targets from heat shock stress. View Full-Text
Keywords: ankyrin; tetratricopeptide; heat shock tolerant; holdase chaperone; structural switching ankyrin; tetratricopeptide; heat shock tolerant; holdase chaperone; structural switching
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Paeng, S.K.; Kang, C.H.; Chi, Y.H.; Chae, H.B.; Lee, E.S.; Park, J.H.; Wi, S.D.; Bae, S.B.; Phan, K.A.T.; Lee, S.Y. AtTPR10 Containing Multiple ANK and TPR Domains Exhibits Chaperone Activity and Heat-Shock Dependent Structural Switching. Appl. Sci. 2020, 10, 1265.

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