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Article

Enzymatic Transesterification of Waste Frying Oil from Local Restaurants in East Colombia Using a Combined Lipase System

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Agricultural and Environmental Sciences Department, Universidad Francisco de Paula Santander (UFPS), Cúcuta 12E-96, Colombia
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Grupo de Investigación Eureka UDES, Facultad de Ingenierías, Universidad de Santander, Avenida 4 No., Cúcuta 10N-61, Colombia
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Biotechnology and Bioengineering Department, CINVESTAV-IPN, Instituto Politécnico Nacional, Mexico City 07360, Mexico
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Nanomaterials and Computer-aided Process Engineering Research Group (NIPAC), Chemical Engineering Department, University of Cartagena, Avenida Consulado Cll. 30 No.48-152, Cartagena 130015, Colombia
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Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(10), 3566; https://doi.org/10.3390/app10103566
Received: 19 October 2019 / Revised: 22 February 2020 / Accepted: 1 March 2020 / Published: 21 May 2020
(This article belongs to the Section Chemical and Molecular Sciences)
The search for innovation and biotechnological strategies in the biodiesel production chain have become a topic of interest for scientific community owing the importance of renewable energy sources. This work aimed to implement an enzymatic transesterification process to obtain biodiesel from waste frying oil (WFO). The transesterification was performed by varying reaction times (8 h, 12 h and 16 h), enzyme concentrations of lipase XX 25 split (14%, 16% and 18%), pH of reaction media (6, 7 and 8) and reaction temperature (35, 38 and 40 °C) with a fixed alcohol–oil molar ratio of 3:1. The optimum operating conditions were selected to quantify the amount of fatty acid methyl esters (FAMEs) generated. The highest biodiesel production was reached with an enzyme concentration of 14%, reaction time of 8 h, pH of 7 and temperature of 38 °C. It was estimated a FAMEs production of 42.86% for the selected experiment; however, best physicochemical characteristics of biodiesel were achieved with an enzyme concentration of 16% and reaction time of 8 h. Results suggested that enzymatic transesterification process was favorable because the amount of methyl esters obtained was similar to the content of fatty acids in the WFO. View Full-Text
Keywords: biodiesel; transesterification; waste frying oil; biotechnology biodiesel; transesterification; waste frying oil; biotechnology
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MDPI and ACS Style

Ferreira Vela, M.A.; Acevedo-Páez, J.C.; Urbina-Suárez, N.; Rangel Basto, Y.A.; González-Delgado, Á.D. Enzymatic Transesterification of Waste Frying Oil from Local Restaurants in East Colombia Using a Combined Lipase System. Appl. Sci. 2020, 10, 3566. https://doi.org/10.3390/app10103566

AMA Style

Ferreira Vela MA, Acevedo-Páez JC, Urbina-Suárez N, Rangel Basto YA, González-Delgado ÁD. Enzymatic Transesterification of Waste Frying Oil from Local Restaurants in East Colombia Using a Combined Lipase System. Applied Sciences. 2020; 10(10):3566. https://doi.org/10.3390/app10103566

Chicago/Turabian Style

Ferreira Vela, Mary Angélica, Juan C. Acevedo-Páez, Nestor Urbina-Suárez, Yeily Adriana Rangel Basto, and Ángel Darío González-Delgado. 2020. "Enzymatic Transesterification of Waste Frying Oil from Local Restaurants in East Colombia Using a Combined Lipase System" Applied Sciences 10, no. 10: 3566. https://doi.org/10.3390/app10103566

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