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Article

LDLR Gene Polymorphisms (rs5925 and rs1529729) Are Associated with Susceptibility to Coronary Artery Disease in a South Indian Population

1
Department of Human Genetics, Punjabi University, Punjab 147002, India
2
Department of Medical Lab Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia
3
Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia
4
Sri Jayadeva Institute of Cardiovascular Science and Research, Bangalore 560069, India
*
Author to whom correspondence should be addressed.
Med. Sci. 2019, 7(7), 80; https://doi.org/10.3390/medsci7070080
Received: 9 June 2019 / Revised: 7 July 2019 / Accepted: 8 July 2019 / Published: 15 July 2019
(This article belongs to the Section Cardiovascular Disease)
Cardiovascular diseases (CVD) are a major cause of death in India and worldwide. Atherosclerosis is caused by the interaction of environmental and genetic factors. Hypercholesterolemia is an example of a classical risk factor for CVD. The low-density lipoprotein receptor (LDLR) is one of the regulating mechanisms the liver uses for cholesterol homeostasis. Gene variations in the LDLR have been reported to cause hypercholesterolemia and consequently CVD. We investigated the association of polymorphisms in the LDLR (rs5925 and rs1529729) with coronary artery disease (CAD) in 200 coronary artery disease patients and 200 matched healthy controls using allele-specific PCR (AS-PCR). The results indicated that the CT genotype of the rs1529729 polymorphism was associated a decreased susceptibility to CAD with an odds ratio (OR) = 0.42 (95% confidence interval (CI), 0.23–0.77), risk ratio (RR) = 0.59 (0.39–0.89), P = 0.0047. The TT genotype of the rs1529729 polymorphism was also associated with decreased susceptibility to CAD with an OR = 0.19 (95% CI, 0.076–0.47), RR = 0.57 (0.47–0.69), P = 0.0003. The GA genotype of the rs5925 polymorphism was associated with decreased susceptibility to CAD with an OR = 0.45 (95% CI, 0.27–0.75), RR = 0.65 (0.47–0.88), P = 0.002. We concluded that the CT and TT genotypes of the rs1529729 polymorphism and the GA genotype of the rs5925 polymorphism are probably associated with decreased susceptibility to CAD. The simplicity of AS-PCR makes it particularly suitable for the rapid, large-scale screening of gene variabilities in the LDLR. AS-PCR could provide significant benefits in clinical applications with its ability to amplify a lower quantity of samples in a cost-saving manner. Nevertheless, these findings need to be validated in well-designed studies with larger sample sizes and in different populations. View Full-Text
Keywords: cardiovascular diseases (CVD); allele-specific-PCR; lipoprotein receptor (LDLR); atherosclerosis; SNPs rs5925 and rs1529729; hypercholesterolemia cardiovascular diseases (CVD); allele-specific-PCR; lipoprotein receptor (LDLR); atherosclerosis; SNPs rs5925 and rs1529729; hypercholesterolemia
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MDPI and ACS Style

K. Jha, C.; Mir, R.; Elfaki, I.; Banu, S.; Chahal, S.M.S. LDLR Gene Polymorphisms (rs5925 and rs1529729) Are Associated with Susceptibility to Coronary Artery Disease in a South Indian Population. Med. Sci. 2019, 7, 80. https://doi.org/10.3390/medsci7070080

AMA Style

K. Jha C, Mir R, Elfaki I, Banu S, Chahal SMS. LDLR Gene Polymorphisms (rs5925 and rs1529729) Are Associated with Susceptibility to Coronary Artery Disease in a South Indian Population. Medical Sciences. 2019; 7(7):80. https://doi.org/10.3390/medsci7070080

Chicago/Turabian Style

K. Jha, Chandan, Rashid Mir, Imadeldin Elfaki, Shaheena Banu, and S. M.S. Chahal. 2019. "LDLR Gene Polymorphisms (rs5925 and rs1529729) Are Associated with Susceptibility to Coronary Artery Disease in a South Indian Population" Medical Sciences 7, no. 7: 80. https://doi.org/10.3390/medsci7070080

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