Leptin Gene G2548A Polymorphism among Mongolians with Metabolic Syndrome
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Subjects
2.2. Biochemical Parameters
2.3. Genotyping of Single-Nucleotide Polymorphisms
2.4. Statistical Analysis
3. Results
3.1. Clinical Data and Biochemical Parameters
3.2. Allele Frequency of Single-Nucleotide Polymorphisms
3.3. Association of Single-Nucleotide Polymorphisms with Biochemical Parameters
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Parameters | MetS (n = 160) | Control (n = 144) | p-Value |
---|---|---|---|
Age (years old) | 41.7 ± 11.3 | 41.2 ± 10.2 | 0.924 |
Gender (M/F) | 86/74 | 71/73 | 0.527 |
BMI (kg/m2) | 31.27 ± 4.23 | 26.64 ± 3.75 | <0.001 |
WC (cm) | 100.97 ± 1.10 | 89.01 ± 12.75 | <0.001 |
SBP (mmHg) | 128.75 ± 13.97 | 114.02 ± 14.44 | <0.001 |
DBP (mmHg) | 88.42 ± 9.92 | 77.95 ± 9.52 | <0.001 |
FBG (mg/dL) | 92.07 ± 66.57 | 71.69 ± 12.69 | 0.012 |
TG (mg/dL) 1 | 124.45 (83.74–179.37) | 66.37 (49.52–96.35) | <0.001 |
TC (mg/dL) | 157.72 ± 36.42 | 148.47 ± 36.73 | 0.123 |
HDL-C (mg/dL) | 32.04 ± 11.49 | 36.44 ± 15.63 | 0.048 |
LDL-C (mg/Dl) | 96.85 ± 40.38 | 95.75 ± 39.53 | 0.867 |
Insulin (ng/mL) 1 | 12.06 (0.29–113.53) | 8.53 (0.29–170.29) | 0.015 |
HOMA-IR 1 | 2.28 (0.05–38.99) | 1.43 (0.05–36.32) | 0.006 |
Adiponectin (ng/mL) 1 | 6.46 (0.06–19.51) | 6.18 (0.09–49.66) | 0.936 |
Leptin (ng/mL) 1 | 11.10 (2.30–56.30) | 4.5 (0.01–34.87) | <0.001 |
Gene | SNPs | Genotype | MetS Group % (n) | Control Group % (n) | p-Value |
---|---|---|---|---|---|
LEP | G2548A | GG | 61.2% (98) | 68.7% (99) | 0.035 |
GA | 33.12% (53) | 29.8% (43) | |||
AA | 5.6% (9) | 1.4% (2) | |||
G | 77.8% (249) | 83.7% (241) | 0.104 | ||
A | 21.2% (71) | 16.3% (47) | |||
LEPR | A668G (Gln223Arg) | AA | 69.4% (111) | 56.9% (82) | 0.053 |
AG | 26.8% (43) | 36.1% (52) | |||
GG | 3.7% (6) | 6.9% (10) | |||
A | 82.81% (265) | 75.0% (216) | 0.018 | ||
G | 17.19% (55) | 25.0% (72) |
Gene/SNP | Genotype | MetS Group % (n) | Control Group % (n) | OR (95% CI) 1 | OR (95% CI) 2 |
---|---|---|---|---|---|
LEP G2548A | GG | 61.2% (98) | 68.7% (99) | 1 | 1 |
GA | 33.12% (53) | 29.8% (43) | 1.22 (0.69–1.86) | 1.31 (0.62–1.61) | |
AA | 5.6% (9) | 1.4% (2) | 3.23 * (1.30–18.51) | 2.78 * (1.06–7.59) | |
GG/GA + AA | 38.7% (62) | 31.25% (45) | 2.18 (0.94–3.16) | 1.72 (0.63–2.13) | |
GG+GA/AA | 7.50% (9) | 1.39% (2) | 2.94 (1.08–17.25) | 2.16 (0.93–7.16) |
Combination | LepR G223A | Lep G2548A | Frequency % (n) | OR 1 (95% CI) | p-Value | |
---|---|---|---|---|---|---|
MetS (n = 160) | Control (n = 144) | |||||
1 | AA | GG | 43.7 (70) | 38.9 (55) | 1.03 (0.42–1.78) | 0.487 |
2 | AA | GA | 20 (31) | 0.16 (24) | 1.12 (0.38–1.82) | 0.245 |
3 | AA | AA | 6.25 (10) | 0.01 (2) | 2.86 (1.0–20.79) | 0.051 |
4 | AG | GG | 0.15 (24) | 0.23 (35) | 0.51 (0.29–1.47) | 0.062 |
5 | AG | GA | 0.10 (16) | 0.12 (18) | 0.64 (0.31–1.50) | 0.845 |
6 | AG | AA | 0.025 (3) | - | - | - |
7 | GG | GG | 0.025 (4) | 0.05 (8) | 0.36 (0.09–1.32) | 0.285 |
8 | GG | GA | 0.01 (1) | 0.01 (2) | 0.74 (0.05–1.84) | 0.113 |
9 | GG | AA | - | - | - | - |
Clinical Features | Model 1 | Model 2 | ||||
---|---|---|---|---|---|---|
GG | GA + AA | p-Value | GG + GA | AA | p-Value | |
BMI (kg/M2) | 31.13 ± 4.00 | 31.49 ± 4.56 | 0.608 | 30.85 ± 4.04 | 36.52 ± 2.49 | <0.001 |
WC (cm) | 99.30 ± 10.37 | 102.03 ± 11.41 | 0.130 | 100.53 ± 11.24 | 106.41 ± 6.94 | 0.077 |
BP, systolic (mmHg) | 129.69 ± 15.17 | 127.25 ± 11.65 | 0.283 | 128.64 ± 13.90 | 130.0 ± 14.77 | 0.748 |
BP, diastolic (mmHg) | 87.65 ± 10.89 | 89.64 ± 7.98 | 0.216 | 88.17 ± 10.05 | 91.50 ± 7.22 | 0.264 |
TC (mg/dL) | 156.70 ± 39.08 | 159.29 ± 31.78 | 0.663 | 157.76 ± 36.75 | 157.20 ± 36.56 | 0.960 |
TG (mg/dL) 1 | 129.20 (89.61–183.1) | 105.50 (81.09–174.8) | 0.828 | 128.95 (86.74–174.80) | 101.76 (75.45–185.40) | 0.697 |
HDL (mg/dL) | 33.89 ± 13.26 | 30.86 ± 10.05 | 0.104 | 34.02 ± 13.82 | 33.27 ± 10.75 | 0.681 |
LDL (mg/dL) | 96.90 ± 36.95 | 93.97 ± 43.18 | 0.650 | 96.12 ± 38.78 | 96.05 ± 32.98 | 0.957 |
FBG (ng/mL) 1 | 75.37 (60.50–80.57) | 77.95 (68.13–95.46) | 0.014 | 75.59 (65.91–88.40) | 80.65 (70.78–107.90) | 0.228 |
Adiponectin (ng/mL) 1 | 6.33 (0.060–19.51) | 6.51 (1.34–12.91) | 0.572 | 6.30 (4.48–8.93) | 8.26 (6.36–10.61) | 0.067 |
Leptin (ng/mL) 1 | 9.5 (2.3–38.0) | 16.1 (3.5–56.3) | 0.011 | 9.7 (6.7–21.6) | 43.65 (36.0–45.8) | 0.001 |
Insulin 1 (µIU/mL) | 12.06 (0.29–84.71) | 12.94 (1.18–113.53) | 0.861 | 11.76 (7.35–1.71) | 13.97 (13.24–95.0) | 0.120 |
HOMA-IR 1 | 2.37 (0.05–38.99) | 2.20 (0.20–25.31) | 0.344 | 2.24 (1.19–4.0) | 8.4 (1.64–22.77) | 0.108 |
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Dagdan, B.; Chuluun-Erdene, A.; Sengeragchaa, O.; Malchinkhuu, M.; Janlav, M. Leptin Gene G2548A Polymorphism among Mongolians with Metabolic Syndrome. Med. Sci. 2019, 7, 3. https://doi.org/10.3390/medsci7010003
Dagdan B, Chuluun-Erdene A, Sengeragchaa O, Malchinkhuu M, Janlav M. Leptin Gene G2548A Polymorphism among Mongolians with Metabolic Syndrome. Medical Sciences. 2019; 7(1):3. https://doi.org/10.3390/medsci7010003
Chicago/Turabian StyleDagdan, Batnaran, Ariunbold Chuluun-Erdene, Orgil Sengeragchaa, Munkhzol Malchinkhuu, and Munkhtsetseg Janlav. 2019. "Leptin Gene G2548A Polymorphism among Mongolians with Metabolic Syndrome" Medical Sciences 7, no. 1: 3. https://doi.org/10.3390/medsci7010003