Genomic Identification and Expression Analysis of the Cathelicidin Gene Family of the Forest Musk Deer
Institute of Ecology, Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong 637009, China
Sichuan Wildlife Rehabilitation and Breeding Research Center, China West Normal University, Nanchong 637009, China
Chongqing Engineering Technology Research Center for GAP of Genuine Medicinal Materials, Chongqing Institute of Medicinal Plant Cultivation, Nanchuan 408435, China
State Key Laboratory for Quality and Safety of Agro-products (in prepared), Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Authors to whom correspondence should be addressed.
These authors are contributed equally to this work.
Received: 24 May 2019 / Revised: 21 July 2019 / Accepted: 22 July 2019 / Published: 24 July 2019
Cathelicidins are a group of host defense peptides in vertebrates with both antimicrobial and immunomodulatory activities. In the present study, we identified the entire repertoire of the cathelicidin gene family from the forest musk deer genome. Sequence comparison, phylogenetic topology, and gene and genomic organizations collectively suggest that all cathelicidin genes have already been fixed in the genome of forest musk deer before the split of moschidae and bovidae, while independent pseudogenization events have occurred after species divergence. In addition, real-time PCR analysis suggested that all functional cathelicidins play important roles in the immune system. The results of this study will be helpful for further evolutionary and functional studies.