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Open AccessArticle

Detection of Bovine TMEM95 p.Cys161X Mutation in 13 Chinese Indigenous Cattle Breeds

by Sihuan Zhang 1,2,3,†, Kun Peng 3,†, Guoliang Zhang 1,2, Yang Cao 1,2, Meng Zhang 3, Hong Chen 3, Chuzhao Lei 3, Xianyong Lan 3,* and Yumin Zhao 1,2,*
1
Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences, Changchun 130033, China
2
Key Laboratory of Beef Cattle Genetics and Breeding in Ministry of Agriculture and Rural Affairs, Changchun 130033, China
3
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work as co-first author.
Animals 2019, 9(7), 444; https://doi.org/10.3390/ani9070444
Received: 11 May 2019 / Revised: 1 July 2019 / Accepted: 8 July 2019 / Published: 16 July 2019
(This article belongs to the Section Animal Genetics and Genomics)
Chinese indigenous cattle are economically important cattle breeds for animal husbandry development. The promotion and development of Chinese cattle breeds is essential. A previous study found that a nonsense mutation (rs378652941, c.483C > A, p.Cys161X) in bovine transmembrane protein 95 gene (TMEM95) seriously reduced reproductive performance in male Fleckvieh cattle; therefore, this locus was considered a candidate genetic marker in bovine marker-assisted selection (MAS) breeding. Until now, no study has identified this mutation in Chinese cattle breeds. Herein, we detected this c.483C > A mutation in 13 Chinese cattle breeds. Importantly, we found that this mutation did not exist at this locus in our analyzed breeds. Interestingly, we first identified a frameshift insertion/deletion (indel) mutation (NC_037346.1: g.27056998_27057000delCT) in the bovine TMEM95 gene in 11 cattle breeds. Together, the results of this study suggest that the mutation c.483C > A cannot serve as a genetic marker for molecular breeding among Chinese indigenous cattle breeds.
Chinese indigenous cattle breeds have abundant genetic resources, which are valuable for the molecular breeding of cattle around the world. Thus, identifying important candidate genes and their genetic markers is very important for cattle molecular breeding. A previous study found that a nonsense mutation (rs378652941, c.483C > A, p.Cys161X) in the bovine transmembrane protein 95 gene (TMEM95) seriously reduced the reproductive performance in bulls, but few studies have detected this mutation in Chinese indigenous cattle breeds. Since the mutation c.483C > A may serve as a potential genetic marker for selecting higher fertility bulls, in the present study, using tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR), forced PCR restriction fragment length polymorphism (forced PCR-RFLP), and DNA sequencing methods, the mutation c.483C > A was detected in 765 individuals from 13 Chinese cattle breeds. However, the results showed that this mutation did not exist at this locus in our analyzed breeds. Interestingly, we identified a newly frameshift insertion/deletion (indel) mutation (NC_037346.1: g.27056998_27057000delCT) in the bovine TMEM95 gene in 11 cattle breeds, which changed the location of the termination codon and changed the 16 amino acids in the C-terminal to 21 amino acids. Combined with previous studies, our study provides evidence that in Chinese cattle breeds the mutation c.483C > A cannot be used as a genetic marker in molecular breeding. View Full-Text
Keywords: Chinese indigenous cattle; molecular breeding; transmembrane protein 95 gene (TMEM95); male reproductive performance Chinese indigenous cattle; molecular breeding; transmembrane protein 95 gene (TMEM95); male reproductive performance
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Zhang, S.; Peng, K.; Zhang, G.; Cao, Y.; Zhang, M.; Chen, H.; Lei, C.; Lan, X.; Zhao, Y. Detection of Bovine TMEM95 p.Cys161X Mutation in 13 Chinese Indigenous Cattle Breeds. Animals 2019, 9, 444.

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