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Open AccessArticle

Different Diets Change the Expression of Bovine Serum Extracellular Vesicle-miRNAs

by Suyu Quan 1,2,†, Xuemei Nan 1,†, Kun Wang 1, Linshu Jiang 3, Junhu Yao 2,* and Benhai Xiong 1,*
1
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
College of Animal Science and Technology, Northwest A&F University, Yanglin 712100, China
3
Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Animals 2019, 9(12), 1137; https://doi.org/10.3390/ani9121137
Received: 2 November 2019 / Revised: 6 December 2019 / Accepted: 10 December 2019 / Published: 13 December 2019
(This article belongs to the Section Animal Physiology)
Studies over the last decade have shown that cells can communicate with neighboring or distant cells through complex packets stuffed with selected proteins, lipids, and nucleic acids, called extracellular vesicles. The wrapped macromolecules are miRNAs, which play a central role in mediating the signal communication of creatural patho/physiological systems. Extracellular vesicle-miRNAs vary among species and different body fluids, such as milk, urine, saliva, cerebrospinal fluid and blood, providing general and individual characters of the vesicles. Cow’s milk is significant in the supply of human nutrition. Therefore, the extracellular vesicle-related physiological process of dairy cows should be of concern. This study clarified the miRNA profiling of bovine serum and found their potential influence on immunity. Moreover, we found that different diets could affect miRNA expression. The results implied that people could implement effective dietary strategies to intervene in the physiological state of animals.
Cells can communicate with neighboring or distant cells using extracellular vesicles (EVs), mainly attributed to their containing miRNAs. Given that diets can change host circulatory miRNA profiling, and EVs are the major miRNA carriers in serum, we hypothesized that different diets could change bovine circulating EV-miRNA expression. We partly replaced alfalfa hay with whole cotton seed and soybean hull in the feed formula of the tested cows. Blood EVs were isolated using a polyethylene glycol precipitation kit. Particle size analysis revealed exosomes were dominant in bovine serum EVs. Small RNAs were enriched in bovine serum EVs, including miRNAs, snRNAs, tiRNAs, Cis-regulatory elements, piRNAs, etc. In total, 359 types of Bos taurus miRNAs were identified by Solexa sequencing. Each cow in the control group contained about 244 types of serum EV-miRNAs, compared to 246 types in the tested group. There were 15 immune-related miRNAs in the top 20 serum EV-miRNAs, accounting for about 80% of the total. Seven differently expressed known miRNAs were detected in responding to different diets. An analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) showed differently expressed miRNAs were related to hormone signal pathways and protein metabolism. Bovine serum EVs are abundant with miRNAs, most of which are immune-related. Different diets eventually change the miRNA profiling of bovine serum EVs. View Full-Text
Keywords: feed formula; nonforage fiber source; blood; exosome feed formula; nonforage fiber source; blood; exosome
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MDPI and ACS Style

Quan, S.; Nan, X.; Wang, K.; Jiang, L.; Yao, J.; Xiong, B. Different Diets Change the Expression of Bovine Serum Extracellular Vesicle-miRNAs. Animals 2019, 9, 1137.

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