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Article

Characteristics of the BMP7 Promoter in Hu Sheep

1
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
2
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
3
Animal Science and Veterinary Medicine Bureau of Suzhou City, Suzhou 215200, China
4
Suzhou Stud Farm, Suzhou 215200, China
5
Small Ruminant Genomics Group, International Center for Agricultural Research in the Dry Areas (ICARDA), Addis Ababa P.O. BOX 5689, Ethiopia
*
Authors to whom correspondence should be addressed.
Animals 2019, 9(11), 874; https://doi.org/10.3390/ani9110874
Received: 28 June 2019 / Revised: 25 September 2019 / Accepted: 11 October 2019 / Published: 28 October 2019
(This article belongs to the Section Small Ruminants)
Bone morphogenetic protein 7 (BMP7) is one of the largest secretory signal conductive molecules and is in the TGF-β superfamily. It plays an important role in the growth and development of hair follicles. We cloned the proximal promoter of the BMP7 gene for bioinformatics analysis. Dual-luciferase reporter system and overexpression were used to analyze the key regions and transcription factor binding sites. There was high activity between −758 bp and −545 bp in the core region of the gene and a possible binding site for transcription factors SP1 and EGR1.
The BMP7 gene is involved in the growth and development of hair follicles but its regulation mechanism is unclear. We studied the regulation mechanism of the BMP7 promoter by cloning the proximal promoter of BMP7 for bioinformatics analysis. A series of missing vectors was then constructed for dual-fluorescein activity detection based on the bioinformatics analysis results. We tested transcription-factor binding-site mutations and transcription factor over-expression to analyze the transcriptional regulation principle of the BMP7 promoter region. The upstream transcriptional regulatory region of the BMP7 gene proximal promoter was predicted by bioinformatics. There were −1216 bp to −1166 bp and −632 bp to −582 bp transcription initiation sites in the upstream transcriptional regulatory region of the BMP7 gene proximal promoter. The CpG islands’ distribution showed that there were many CpG islands at −549 bp to 1 bp. A dual-luciferase assay revealed high activity between −758 bp and −545 bp in the core region and a possible binding site for transcription factors SP1 and EGR1. The transcriptional activity of BMP7 was significantly decreased in the transcriptional regulatory region of the BMP7 after EGR1 and SP1 mutation. Transcription was significantly enhanced by over expression of the EGR1 transcription factor, which strongly suggests that EGR1 and SP1 play important roles in BMP7 regulation. View Full-Text
Keywords: Hu sheep; BMP7; promoter; transcription factor Hu sheep; BMP7; promoter; transcription factor
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MDPI and ACS Style

Lv, X.; Sun, W.; Zou, S.; Chen, L.; Mwacharo, J.M.; Wang, J. Characteristics of the BMP7 Promoter in Hu Sheep. Animals 2019, 9, 874. https://doi.org/10.3390/ani9110874

AMA Style

Lv X, Sun W, Zou S, Chen L, Mwacharo JM, Wang J. Characteristics of the BMP7 Promoter in Hu Sheep. Animals. 2019; 9(11):874. https://doi.org/10.3390/ani9110874

Chicago/Turabian Style

Lv, Xiaoyang, Wei Sun, Shuangxia Zou, Ling Chen, Joram M. Mwacharo, and Jinyu Wang. 2019. "Characteristics of the BMP7 Promoter in Hu Sheep" Animals 9, no. 11: 874. https://doi.org/10.3390/ani9110874

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