Next Article in Journal
Addendum: Gosling, E.M., et al. The Status of Pet Rabbit Breeding and Online Sales in the UK: A Glimpse into an Otherwise Elusive Industry. Animals 2018, 8, 199
Previous Article in Journal
Increasing Maximum Penalties for Animal Welfare Offences in South Australia—Has It Caused Penal Change?
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Animals 2018, 8(12), 237; https://doi.org/10.3390/ani8120237

Influence of Liver Condition and Copper on Selective Parameters of Post-Mortem Dog Tissue Samples

1
Department of Grain Science & Industry, Kansas State University, Manhattan, KS 66506, USA
2
Nutrition Innovation, Hill’s Pet Nutrition, Topeka, KS 66601, USA
*
Author to whom correspondence should be addressed.
Received: 5 October 2018 / Revised: 3 December 2018 / Accepted: 5 December 2018 / Published: 13 December 2018
(This article belongs to the Section Companion Animals)
Full-Text   |   PDF [371 KB, uploaded 13 December 2018]   |  
  |   Review Reports

Simple Summary

The liver is a vital organ involved in numerous physiological functions. Maintaining its health is vital to the wellbeing of the dog. Copper is a transition metal that can cause cell oxidation when stored in excess. This surplus storage in the liver may happen due to breed genetics, or excess dietary copper consumption. The objective of this work was to determine relationship between hepatic copper and liver pathology severity with plasma metabolites, complete blood count, and blood chemistry. Copper accumulation was not related to either liver pathological condition nor to an increase in liver biomarkers in the selected dog population. However, an increasing copper concentration suggested oxidation and cell membrane stress. Liver pathology severity was related to increased liver enzymes, and some cholestasis. Further, liver neoplasia was correlated with biomarkers that suggest rapid cell division and increased energy metabolism.

Abstract

One of the liver functions is copper storage, which can be toxic when in excess. The objective of this retrospective study was to determine the relationship between hepatic copper and pathology conditions in stored samples from 55 post-mortem dogs (37 Beagles, 12 Labrador Retrievers, and 6 Labrador Mixes). The analyses evaluated data from blood chemistry and complete blood count (CBC) that were measured immediately before euthanasia, and liver biopsies which were harvested at necropsy and frozen at −80 °C. Slides for microscopic evaluation were prepared, and liver copper and plasma metabolites were measured. Hepatic copper was correlated (p ≤ 0.001) with monoacylglycerols, 13-HODE + 9-HODE (13-hydroxy-9,11-octadecadienoic acid + 9-hydroxy-10,12-octadecadienoic acid), and stearoyl-arachidonoyl-glycerophosphocholine. This indicates lipid metabolism modification and cell membrane oxidation. However, hepatic copper was not related to liver histopathology severity or altered liver biomarkers. The severity of liver pathology was positively correlated (p ≤ 0.05) with liver enzymes, bile salts, and glycerophosphocholines, suggesting cholestasis and altered lipid and amino acid metabolism. Liver neoplasia had increased (p ≤ 0.05) metabolites derived from nucleotides, along with an increase (p ≤ 0.05) in α-ketoglutarate from the energy and amino acid metabolism (p ≤ 0.05), suggesting rapid cell division. This study offers further insight regarding changes in metabolism due to hepatic tissue damage. View Full-Text
Keywords: dog; liver; copper; histopathology; cholestasis; oxidation dog; liver; copper; histopathology; cholestasis; oxidation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Corsato Alvarenga, I.; Aldrich, C.G.; Jewell, D.E. Influence of Liver Condition and Copper on Selective Parameters of Post-Mortem Dog Tissue Samples. Animals 2018, 8, 237.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Animals EISSN 2076-2615 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top