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Extending the Collection Duration of Breath Samples for Enteric Methane Emission Estimation Using the SF6 Tracer Technique
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Methane Emission and Milk Production of Dairy Cows Grazing Pastures Rich in Legumes or Rich in Grasses in Uruguay

Facultad de Agronomía, Universidad de la República, Av. Garzón 780, Montevideo 12900, Uruguay
IFAS, Facultad de Ciencias Exactas, UNCPBA, CONICET, Pinto 399, Tandil 7000, Argentina
INFQC, Universidad de Córdoba, CONICET, Av. Haya de la Torre s/n, Ciudad Universitaria, Córdoba 5000, Argentina
Author to whom correspondence should be addressed.
Animals 2012, 2(2), 288-300;
Received: 9 May 2012 / Revised: 31 May 2012 / Accepted: 6 June 2012 / Published: 8 June 2012
(This article belongs to the Special Issue Climate Change and Livestock Management)
PDF [88 KB, uploaded 8 June 2012]

Simple Summary

GHGs emissions are relevant in evaluating environmental impact of farming systems. Methane (CH4) produced by enteric fermentation accounts for half of all anthropogenic emissions of GHGs in Uruguay, where ruminant production is based on year round grazing of forages. Here we compared milk production and CH4 emissions by dairy cows grazing two contrasting mixed pastures (rich in legumes or rich in grasses) using the SF6 tracer technique adapted to collect breath samples over 5-days periods. There were no differences in milk or CH4 production between the contrasting pastures, probably because of the high herbage allowance that enabled selective grazing by cows.


Understanding the impact of changing pasture composition on reducing emissions of GHGs in dairy grazing systems is an important issue to mitigate climate change. The aim of this study was to estimate daily CH4 emissions of dairy cows grazing two mixed pastures with contrasting composition of grasses and legumes: L pasture with 60% legumes on Dry Matter (DM) basis and G pasture with 75% grasses on DM basis. Milk production and CH4 emissions were compared over two periods of two weeks during spring using eight lactating Holstein cows in a 2 × 2 Latin square design. Herbage organic matter intake (HOMI) was estimated by chromic oxide dilution and herbage organic matter digestibility (OMD) was estimated by faecal index. Methane emission was estimated by using the sulfur hexafluoride (SF6) tracer technique adapted to collect breath samples over 5-day periods. OMD (0.71) and HOMI (15.7 kg OM) were not affected by pasture composition. Milk production (20.3 kg/d), milk fat yield (742 g/d) and milk protein yield (667 g/d) were similar for both pastures. This may be explained by the high herbage allowance (30 kg DM above 5 cm/cow) which allowed the cows to graze selectively, in particular in grass sward. Similarly, methane emission expressed as absolute value (368 g/d or 516 L/d) or expressed as methane yield (6.6% of Gross Energy Intake (GEI)) was not affected by treatments. In conclusion, at high herbage allowance, the quality of the diet selected by grazing cows did not differ between pastures rich in legumes or rich in grasses, and therefore there was no effect on milk or methane production. View Full-Text
Keywords: dairy cows; grazing; intake; digestibility; methane; SF6 dairy cows; grazing; intake; digestibility; methane; SF6
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Dini, Y.; Gere, J.; Briano, C.; Manetti, M.; Juliarena, P.; Picasso, V.; Gratton, R.; Astigarraga, L. Methane Emission and Milk Production of Dairy Cows Grazing Pastures Rich in Legumes or Rich in Grasses in Uruguay. Animals 2012, 2, 288-300.

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