Comparison of Intestinal Bacteria of Procambarus clarkii Farmed in Various Rice Paddy Regions
by
, , , and
Chonghang Ding
1,†,
Rui Jia
2,†,
Yunfeng Wang
3,
Yiran Hou
2
,
Liqiang Zhang
2,
Bing Li
2,* and
Jian Zhu
1,2,* 1
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
2
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
3
Fishery Technology Extension Station of Yunnan, No. 25 Dianchi Road, Xishan District, Kunming 650034, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Animals 2024, 14(6), 935; https://doi.org/10.3390/ani14060935
Submission received: 26 February 2024
/
Revised: 13 March 2024
/
Accepted: 14 March 2024
/
Published: 19 March 2024
(This article belongs to the Special Issue Crustaceans Biology, Physiology, Reproduction and Development)
Simple Summary
Procambarus clarkii, an important freshwater species in China, is predominantly cultivated in an integrated rice–crayfish co-culture system. However, potential regional differences in the quality of P. clarkii have been scarcely reported. Therefore, the aim of this study was to assess the regional differences of P. clarkii through analyzing gut microbiota composition in specimens from ten major producing areas in China. Microbial sequencing analysis demonstrated that P. clarkii across various regions exhibit distinct microbial diversity and composition. These data would contribute to the assessment of the quality of P. clarkii aquaculture.
Abstract
The aim of this study was to assess the regional differences of Procambarus clarkii through analyzing gut microbiota in specimens from different areas in China. The P. clarkii were collected from ten integrated rice–crayfish farming systems locating across ten major producing areas as follows: Feixi (FX), Suqian (SQ), Yangzhou (YZ), Xuyi (XY), Qianjiang (QJ), Jianli (JL), Honghu (HH), Yueyang (YY), Changsha (CS), and Nanxian (NX). The composition of gut microbiota was assessed by analyzing 16S rRNA sequences. The PCoA results indicated significant differences in microbial community composition among the ten areas (R = 0.999, p = 0.001). The intestinal microbial diversity in P. clarkii cultured in rice fields from YY and CS exceeded that of other regions, with NX displaying the least diversity. At the phylum level, Proteobacteria were most abundant in HH, while Firmicutes showed increased relative abundances in FX and SQ, contrasted by lower relative abundances of Bacteroidetes in these areas. At the genus level, Ralstonia, Amedibacillus, Bacteroides, Anaerorhabdus, and Dysgonomonas were the dominant bacteria. The bacterial co-occurrence networks analysis revealed that the community structures in locations FX, SQ, XY, HH, and NX were comparatively simplistic, whereas those in the YZ, QJ, JL, YY, and CS regions displayed as more complex. In summary, the diversity and relative abundance of intestinal bacteria exhibits regional variability. These findings can offer theoretical data for evaluating the quality of P. clarkii aquaculture.
