Isolation and Molecular Characterisation of Respirovirus 3 in Wild Boar
Abstract
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample
2.2. Viral Isolation
2.3. Negative Staining Electron Microscopy (nsEM)
2.4. Immunofluorescence (IF) Test
2.5. Antigen Detection Enzyme-Linked Immunosorbent Assay (ELISA)
2.6. Hemagglutination Assay (HA)
2.7. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Sequencing
Phylogenetic Analysis of Partial M Gene
2.8. Whole-Genome Sequence
Phylogenetic Analysis
3. Results
3.1. Morphological and Biological Properties of Wild Boar Isolate
3.2. Molecular Characterisation RT-PCR and Sequencing
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Virus | Strain | F | HN | ||
---|---|---|---|---|---|
Nucleotide (%) | Amino Acid (%) | Nucleotide (%) | Amino Acid (%) | ||
180608_2004 | SPMV-Texas-81 | 100 | 100 | 99.8 | 99.8 |
SPMV-ISU-92 | 92 | 94.8 | 92 | 95.7 | |
BRV3a-SF | 100 | 100 | 99.8 | 99.7 | |
BRV3a-NC002161 | 92.3 | 95 | 92.2 | 95.9 | |
BRV3c-SD0835 | 82.5 | 86.6 | 80.2 | 84 | |
BRV3b-Q5592 | 82.4 | 84.4 | 82.2 | 86.3 | |
HRV3-14702 | 78.4 | 79.5 | 75.2 | 70 |
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Sozzi, E.; Lelli, D.; Barbieri, I.; Chiapponi, C.; Moreno, A.; Trogu, T.; Tosi, G.; Lavazza, A. Isolation and Molecular Characterisation of Respirovirus 3 in Wild Boar. Animals 2023, 13, 1815. https://doi.org/10.3390/ani13111815
Sozzi E, Lelli D, Barbieri I, Chiapponi C, Moreno A, Trogu T, Tosi G, Lavazza A. Isolation and Molecular Characterisation of Respirovirus 3 in Wild Boar. Animals. 2023; 13(11):1815. https://doi.org/10.3390/ani13111815
Chicago/Turabian StyleSozzi, Enrica, Davide Lelli, Ilaria Barbieri, Chiara Chiapponi, Ana Moreno, Tiziana Trogu, Giovanni Tosi, and Antonio Lavazza. 2023. "Isolation and Molecular Characterisation of Respirovirus 3 in Wild Boar" Animals 13, no. 11: 1815. https://doi.org/10.3390/ani13111815
APA StyleSozzi, E., Lelli, D., Barbieri, I., Chiapponi, C., Moreno, A., Trogu, T., Tosi, G., & Lavazza, A. (2023). Isolation and Molecular Characterisation of Respirovirus 3 in Wild Boar. Animals, 13(11), 1815. https://doi.org/10.3390/ani13111815