Dynamic of Hepatitis E Virus (HEV) Shedding in Pigs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Samplings
2.2. RNA Extraction and Quantitative Real-Time Reverse Transcription PCR
2.3. Statistical Analysis
2.4. Sequencing
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Batch | Sampling (T) | Age (Days) | No. of Days from the First Sampling (T1) | HEV-RNA | ||
---|---|---|---|---|---|---|
Total Tested | Positive | Prevalence % | ||||
A | 1 | 110 | 0 | 10 | 10 | 100 |
2 | 122 | 12 | 10 | 10 | 100 | |
3 | 131 | 21 | 10 | 10 | 100 | |
4 | 140 | 30 | 10 | 10 | 100 | |
5 | 153 | 43 | 9 | 8 | 88.9 | |
6 | 166 | 56 | 9 | 6 | 66.7 | |
7 | 181 | 71 | 9 | 0 | 0 | |
8 | 209 | 99 | 7 | 0 | 0 | |
9 | 235 | 125 | 6 | 0 | 0 | |
B | 1 | 90 | 0 | 13 | 13 | 100 |
2 | 117 | 27 | 13 | 13 | 100 | |
3 | 158 | 68 | 13 | 5 | 38.5 | |
4 | 187 | 97 | 13 | 1 | 7.7 |
Age of Animals (Days) | |||||
---|---|---|---|---|---|
Batch | Mean | Std. Dev. | Minimum | Maximum | Median |
A | 143.29 | 23.33 | 110 | 166 | 140.0 |
B | 138.00 | 37.60 | 90 | 187 | 137.5 |
Animals | GC/g 1 | ||||||||
---|---|---|---|---|---|---|---|---|---|
Batch | Sampling (T) | Age (Days) | No. Tested | HEV Positive | Mean 1 | Std. Deviation | Minimum | Maximum | Median |
A | 1 | 110 | 10 | 10 | 5.99 | 1.03 | 4.55 | 7.72 | 5.82 |
2 | 122 | 10 | 10 | 4.89 | 1.15 | 2.91 | 6.53 | 4.60 | |
3 | 131 | 10 | 10 | 4.09 | 0.44 | 3.23 | 4.72 | 4.16 | |
4 | 140 | 10 | 10 | 3.67 | 0.25 | 3.13 | 4.00 | 3.71 | |
5 | 153 | 9 | 8 | 3.14 | 1.25 | 0.00 | 3.97 | 3.73 | |
6 | 166 | 8 | 6 | 2.66 | 2.29 | 0.00 | 5.64 | 3.59 | |
7 | 181 | 9 | 0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
8 | 209 | 7 | 0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
9 | 235 | 6 | 0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
B | 1 | 90 | 13 | 13 | 5.89 | 1.08 | 4.67 | 9.04 | 5.67 |
2 | 117 | 13 | 13 | 4.63 | 0.65 | 3.08 | 5.69 | 4.80 | |
3 | 158 | 13 | 5 | 1.22 | 1.62 | 0.00 | 3.48 | 0.00 | |
4 | 187 | 13 | 1 | 0.25 | 0.90 | 0.00 | 3.25 | 0.00 |
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Ianiro, G.; Monini, M.; De Sabato, L.; Chelli, E.; Cerini, N.; Ostanello, F.; Di Bartolo, I. Dynamic of Hepatitis E Virus (HEV) Shedding in Pigs. Animals 2022, 12, 1063. https://doi.org/10.3390/ani12091063
Ianiro G, Monini M, De Sabato L, Chelli E, Cerini N, Ostanello F, Di Bartolo I. Dynamic of Hepatitis E Virus (HEV) Shedding in Pigs. Animals. 2022; 12(9):1063. https://doi.org/10.3390/ani12091063
Chicago/Turabian StyleIaniro, Giovanni, Marina Monini, Luca De Sabato, Eleonora Chelli, Natalino Cerini, Fabio Ostanello, and Ilaria Di Bartolo. 2022. "Dynamic of Hepatitis E Virus (HEV) Shedding in Pigs" Animals 12, no. 9: 1063. https://doi.org/10.3390/ani12091063
APA StyleIaniro, G., Monini, M., De Sabato, L., Chelli, E., Cerini, N., Ostanello, F., & Di Bartolo, I. (2022). Dynamic of Hepatitis E Virus (HEV) Shedding in Pigs. Animals, 12(9), 1063. https://doi.org/10.3390/ani12091063