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Article

Temporal Changes of Fish Diversity and Driver Factors in a National Nature Reserve, China

by
Dongmei Luo
1,
Ziwei Wan
1,
Chenchen Jia
1,
Yiqiu Zhu
2,
Qin Zou
3,
Fangfei Ye
3,
Yan Feng
3,
Mi Zhang
3,
Xiaoping Wu
1,
Xiongjun Liu
4,* and
Shan Ouyang
1,*
1
School of Life Sciences, Nanchang University, Nanchang 330031, China
2
Aquatic Biology Protection and Rescue Center of Jiangxi Province, Nanchang 330031, China
3
Lushan National Nature Reserve Administration of Jiangxi Province, Nanchang 330031, China
4
Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, School of Life Science, Jiaying University, Meizhou 514015, China
*
Authors to whom correspondence should be addressed.
Animals 2022, 12(12), 1544; https://doi.org/10.3390/ani12121544
Submission received: 19 May 2022 / Revised: 8 June 2022 / Accepted: 10 June 2022 / Published: 14 June 2022
(This article belongs to the Special Issue Aquatic Fauna Biodiversity and Conservation)
Browse Figures
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Abstract

:

Simple Summary

Freshwater-fish diversity declined rapidly due to multiple anthropogenic disturbances. The loss of fish diversity often manifested itself in taxonomic homogenization over time. Knowledge of multi-faceted diversity (i.e., species, functional, and phylogenetic diversity) perspectives is important for biodiversity assessment and conservation planning. The results showed that the diversity of fish has declined from 2008 to 2021, with five species lost over time. We found an overall homogenization trend in the fish fauna of the study area, with a 4% increase in the taxonomic similarity among the rivers. Additionally, we found that the community structure of fish was significantly different among the rivers, and environmental filtering was the main contributor to the phylogenetic diversity of fish in 2008 and 2021. This study provides new insight into the patterns and drivers of fish-biodiversity change in the broader Yangtze River basin and informs management efforts.

Abstract

Freshwater-fish diversity declined rapidly due to multiple anthropogenic disturbances. The loss of fish diversity often manifested itself in taxonomic homogenization over time. Knowledge of multi-faceted diversity (i.e., species, functional, and phylogenetic diversity) perspectives is important for biodiversity assessment and conservation planning. Here, we analyzed the change of the species diversity and phylogenetic diversity of fish in 2008 and 2021 as well as explored the driver factors of the biodiversity patterns in the Lushan National Nature Reserve. The results showed that the species diversity and phylogenetic diversity of fish have declined from 2008 to 2021, with five species lost over time. We found an overall homogenization trend in the fish fauna of the study area, with a 4% increase in taxonomic similarity among the rivers. Additionally, we found that community structure of fish was significantly different among the rivers, and environmental filtering was the main contributor to the phylogenetic diversity of fish in 2008 and 2021. This study provides new insight into the patterns and drivers of fish-biodiversity change in the broader Yangtze River basin and informs management efforts.

1. Introduction

Freshwater fish are one of the most diverse vertebrates and are also one of the most threatened groups globally [1,2,3]. Freshwater fish play major roles in aquatic ecosystems and are indicator organisms for detecting health of the river environment [4,5]. However, freshwater-fish biodiversity declined rapidly due to multiple anthropogenic disturbances, such as dam construction, water pollution, and overfishing [6,7,8]. More than 30% of freshwater-fish populations are declining in the current period [9]. Therefore, knowledge of fish biodiversity is crucial to inform conservation and management strategies.
Anthropogenic disturbances have put these natural environments at risk and affected biodiversity [3,8]. Assessing and quantifying the effect of anthropogenic disturbances on biodiversity is an important topic for ecologists [3,8]. Previous studies have focused on the level of species diversity (e.g., species richness, Shannon–Wiener diversity index) in assessing the effects of anthropogenic disturbances on ecological communities [10,11,12]. In contrast, study on phylogenetic diversity in assessing the effects of anthropogenic disturbances on ecological communities has not received much attention [13,14]. Species diversity reflects treating all species as equal, neglecting the differences in evolutionary relationships among species that may provide complex information of biodiversity [15,16]. Ecologists are increasingly recognizing this shortcoming, and several studies have highlighted the importance of incorporating multifaceted diversity in biodiversity management and conservation planning [17,18,19]. Recently, many studies have focused on phylogenetic diversity because of a few key reasons [19,20,21,22]. Phylogenetic diversity refers to the evolutionary relationships among species, indicating that it can reflect the evolutionary history on biological communities [18,19,20]. In addition, phylogenetic diversity emphasizes ecosystem degradation, compared with species diversity [18,19]. Therefore, knowledge of multi-faceted diversity (i.e., species, functional, and phylogenetic diversity) perspectives is important for biodiversity assessment and conservation planning [20,21,22].
Protected areas can prevent population decline of species and habitat loss, which, therefore, play an important role in protecting biodiversity and species resources [23]. The Lushan National Nature Reserve (29°30′ N–29°41′ N, 115°51′ E–116°07′ E) is located in Jiangxi Province, has abundant biodiversity, and is regarded as a key “biological refuge” in the middle and lower reaches of the Yangtze River in China [24,25]. It has also the titles of a World Heritage Site, 5A Scenic Area, and National Geopark [24,25]. The subtropical forest ecosystem, endangered wildlife species, and habitat are the primary conservation targets in this reserve [24,25]. However, fish habitats and biodiversity have been profoundly affected by human activities, such as tourism activities and water pollution (e.g., domestic sewage and tourist garbage) [25,26]. In the past, Huang et al. [27] have demonstrated the species diversity of fish in the Lushan National Nature Reserve, but few studies have explained the principal mechanisms of the interactions between the species diversity and phylogenetic diversity of fish and anthropogenic disturbances. Here, we aimed to analyze the patterns and drivers of species diversity and phylogenetic diversity of fish in 2008 and 2021 as well as to explore the driver factors of biodiversity patterns in the Lushan National Nature Reserve. This study will provide an important reference for the restoration and conservation of fish biodiversity.

2. Material and Methods

2.1. Study Area

The Lushan National Nature Reserve is an independent mountain in the middle and lower reaches of the Yangtze River, located at the confluence of the Yangtze River and Poyang Lake [26]. It covers a total area of 304.95 km2. Its annual average temperature is 11.4 °C, and annual precipitation is 1917 mm. The mountain stream in the reserve is radially arranged, with the streams on the east slope flowing into Poyang Lake, and the streams on the west slope flowing into Baili Lake. The substrate of the river bed is mainly sand and gravel [27].
The selection of sampling sites in this study considered the habitat variation and anthropogenic activities in the Lushan National Nature Reserve. In total, 13 streams (28 sampling sites) were chosen (Figure 1), including Taohuayuan River (THY), Guizong River (CZ), Xiufeng River (XF), Guanyinqiao River (GYQ), Haihui River (HH), Gaolong River (GL), Tongyuan River (TY), Jiandaoxia River (JDX), Lianhua River (LH), Weijia River (WJ), Shimenjian River (SMJ), Longmengou River (LMG), and Wuliqiao River (WLQ).

2.2. Samplings Methods

The fish-resource surveys were conducted in July and September in 2021. At each site, we first conducted interviews with local people to assess the potential presence of fish, using fish pictures from the Yangtze River. Each site was selected with similar average depths and similar capture efficiencies. Fish sampling was fully standardized using a portable electric fishing machine (CWB-2000 P, China; 12 V import, 250 V export), amounting to a total sampling area of 100 m2. Each site was conducted for 2 h. To enhance the species checklists at each section, we used ground cages (5 m in length, 0.5 m in height, 5 mm in mesh size), gillnets (50 m in length, 2 m in height, 1, 3, 5 cm in mesh size), and hand-held nets to collected fish samples. Fishing gear was exposed for 10 h. Fish samples were counted, measuring body length (cm) and weight (g) in the field. Live fish were released in the study area, and unidentified species were fixed in 10% formaldehyde solution and further identified in the laboratory of Nanchang University. All unidentified fish specimens were deposited at the museum in the School of Life Sciences, Nanchang University. In addition, we surveyed fish resource in the local township markets to enhance the species checklists at each sampling areas. All fish specimens were identified according to Chen (1998) [28], Chu et al. (1999) [29], and Yue (2000) [30], plus Fishbase (http://www.fishbase.org/search.php (accessed on 5 May 2022)) was used to correct the scientific names [31]. The historical fish-species presence and absence in 13 rivers of the Lushan National Nature Reserve were collected from Huang et al. (2008) [27]. Samplings methods and samplings areas of fish in 2008 were the similar to those in this study. The special fishing license required for scientific research has been obtained for this specimen collection, and the sampling has been completed with the assistance of the reserve staff and local fishers.

2.3. Data Analysis

The sampling completeness of fish for the study area was assessed based on abundance-based rarefaction [32]. The abundance-based rarefaction was implemented using confidence intervals (95%) in iNext online [32].
The index of relative importance (IRI: IRI = (%Ni + %Wi) × fi; %Ni and %Wi were percentage of number and percentage of weight, respectively, of species i in the total catches, and %fi was the occurrence frequency of species i) was used to measure the dominant species in the study area [33]. The relative abundance of each species at each sampling site was estimated (Pi = Ni/ ∑ j = 1 s   Nj; S: number of species; Ni and Nj were the counts of individual species in the sample). The Shannon–Weiner index (H: H = −∑(PilnPi)), Margalef diversity index (D: D = (S–1)/lnN), Simpson dominance index (F: F = 1 − ∑(Pi)2), and Pielou evenness index (J: J = H/lnS) were used to analyze fish diversity and richness in each sampled section [34,35]. We analyzed the beta diversity of fish using the Sørensen dissimilarity index (βsor), spatial turnover component (βsim), and nestedness component (βsne) between the fish communities of each pair of sampling sites [36,37]. The analysis of beta diversity was performed in R [38] using the BETAPART package [39] and the VEGAN package [40]. The assemblage structure of fish was analyzed using the non-metric multidimensional scaling (NMDS) and Bray–Curtis similarity index, based on species abundance data, as performed in PRIMER 6 [41]. Before cluster analysis, the original data were converted to the fourth power to lessen the impact of extreme data on the results and bring them closer to a normal distribution. Analysis of similarity (ANOSIM) was used to determine the significance of differences in fish compositions among sampling areas, based on species abundance data, and SIMPER tests were used to determine the contributions of each fish species, based on species abundance data [42].
We downloaded the mitochondrial DNA (mtDNA) Cytb (cytochrome b) sequences of 16 fish species in this study and 17 species in 2008 from NCBI (https://www.ncbi.nlm.nih.gov/ (accessed on 18 February 2022)), the length of the sequences was a 1071 bp sequence. The phylogenetic tree was constructed using the neighbor-joining (NJ) method performed by Megan X. The net relatedness index (NRI) was used to infer the phylogenetic diversity of fish in the Lushan National Nature Reserve. NRI refers to the standardized effect size of the average pairwise phylogenetic distance (MPDobs) of all species actually obtained in the study area, relative to the random value of the null model (MPDnull). NRI is the average value of the phylogenetic distance of all species in study area, and the formula was as follows [43]:
NRI = MPDobs −   mean ( MPDnull ) SD ( MPDnull )
where MPDobs represents the observed value of the average pairwise taxonomic phylogenetic distance, based on the phylogenetic tree branch-length distance matrix, mean(MPDnull) is the average of 999 random MPD values for each community generated by running 999 random assignments of species on the phylogenetic tree for 1000 iterations, and SD(MPDnull) is the standard deviation of these random values. When NRI > 0, the species are aggregated in the phylogenetic structure, the community is composed of closely related species, and the mechanism of community construction is the environmental filtering. When NRI < 0, the species are divergent in the phylogenetic structure, the community is composed of distant species, and the mechanism of community construction is the competition. The NRI was calculated by R [38].

3. Results

3.1. Species Composition of Fish

The species accumulation curves for fish in the study area were close to asymptotic, based on relatively high sampling completeness and estimating Chao I as more than 95% of the study area (Figure S1). In 2021, a total of 1742 individuals were collected in the 13 rivers in the Lushan National Nature Reserve, including 16 species belonging to 4 orders, 8 families, and 16 genera, which was lower than those in 2008 (a total of 1320 individuals, belonging to 4 orders, 8 families, 17 genera, and 17 species; Table S1). Cypriniformes were the most common order in 2008 and 2021 (Table S1). Five fish species were not found in 2021 (spotted steed (Hemibarbus maculatus), amur catfish (Silurus asotus), yellow catfish (Tachysurus fulvidraco), small snakehead (Channa asiatica), and Chinese minnow (Rhynchocypris oxycephala)), plus four fish species were newly recorded (Bamboo fish (Belligobio nummifer), huang tangding (Pseudobagrus ondon), snakehead (Channa argus), and tupo fish (Odontobutis sinensis)). The Chinese red-listed fish species showed that 15 and 13 of the species are of Least Concern (LC) and 2 and 3 of the species are Data Deficient (DD) in 2008 and 2021, respectively (Table S1). No Chinese red-listed fish species identified as threatened or near-threatened to extinction were recorded in 2008 and 2021 (Table S1). All fish are species native to China in 2008 and 2021 (Table S1).
The average body length of the 13 fish species in 2021 was lower than 10 cm, and 3 species were higher than 10 cm (Belligobio nummifer, Channa argus, Asian swamp eel (Monopterus albus)) (Table S2). The average weight of the 15 fish species was lower than 50 g, and 1 species was higher than 50 g (Channa argus) (Table S2). The percentage of number and weight of freshwater minnow (Zacco platypus), makou (Opsariichthys bidens), shrimp goby (Rhinogobius giurinus), and grouper (Acrossocheilus parallens) were higher than those in other species in 2021 (Table S2). The dominant species were Zacco platypus, Rhinogobius giurinus, Acrossocheilus parallens, and Opsariichthys bidens in 2021, based on IRI (Table S2).

3.2. Diversity of Fish

The diversity and abundance of fish in 2021 (H = 1.21, D = 2.01, F = 0.53, J = 0.44) were lower than those in 2008 (H = 1.72, D = 2.22, F = 0.32, J = 0.61; Figure 2). The diversity and abundance of fish in GYQ were higher than those in other rivers in 2021, and the diversity and abundance of fish in WLQ were higher than those in other rivers in 2008. The lowest diversity and abundance of fish were found in TY for 2021 and WJ for 2008. Beta diversity in 2021 (βsor = 0.78) was lower than in 2008 (βsor = 0.79). Changes in beta diversity were predominantly driven by spatial turnover (βsim = 0.67 in 2008; βsim = 0.62 in 2021), compared to species nestedness (βsne = 0.12 in 2008; βsne = 0.16 in 2021; Figure 3). Differences between 2008 and 2021 for the Sørensen dissimilarity index indicated an overall homogenization of species composition over time (Δβsor = 0. 02).
There were 10 rivers (THY, GZ, XF, GYQ, GL, TY, JDX, LH, SMJ, WLQ, and HH) in 2008 and 6 rivers (THY, XF, HH, GL, WJ, and WLQ) in 2021 with NRI > 0, indicating environmental filtering was the main contributor to the community construction of fish (Table 1). There was one river (OTM) in 2008 and six rivers (GYQ, TY, JDX, LH, SMJ, and LMG) in 2021 with NRI < 0, indicating competition was the main contributor to the community construction of fish (Table 1). The NRI of the Lushan National Nature Reserve in 2008 and 2021 was greater than zero, indicating environmental filtering was the main contributor to the community construction of fish (Table 1).

3.3. Community Structure of Fish

The NMDS and Bray–Curtis similarity results showed that the 13 rivers in 2008 and 2021 were both divided into three groups (Figure 4). The A group was WJ; the B group was LH, TY, GL, OTM, and GZ; and the remaining rivers were the C group in 2008. The A group was WJ, the B group was THY, GZ, GYQ, and GL, and the remaining rivers were the C group in 2021. ANOSIM results in 2008 and 2021 showed that community structure of fish among rivers were significantly different (R2 = 0.642 in 2008, R2 = 0.516 in 2021, p < 0.05). SIMPER tests showed that the average dissimilarity between the first group and the second group was the highest in 2021, and the average dissimilarity between the first group and the second group as well as the first group and the third group were both the highest in 2008 (Table 2). The contribution rate of Zacco platypus was the highest among the three groups in both 2008 and 2021 (Table 2).

4. Discussion

Multiple anthropogenic disturbances, such as dam construction, land use, water pollution, and overfishing, affected the diversity of fish in the freshwater ecosystems [3,8,44,45]. In this study, we found that the diversity of fish in the Lushan National Nature Reserve experienced a decline from 2008 to 2021. Five fish species were extirpated in 2021. The phylogenetic diversity of fish assemblages declined over time, which was usually recognized as a negative signal of biodiversity variation [17,18,19]. These results indicated that multiple anthropogenic disturbances have affected the composition and diversity of fish in the study area. Similar results were also reported for other large rivers in China (e.g., the Yangtze River and the Yellow River [14,46,47]) and globally [43,47]. Indeed, in the field investigation, small dams, land use, and water pollution were found in many rivers in the Lushan National Nature Reserve, resulting in the extirpation of fish in this study. Land use and water pollution was found in the Gaolong River, Guizong River, Tongyuan River, and Lianhua River, resulting in the extirpation of Hemibarbus maculatus and Rhynchocypris oxycephala. Fish assemblages are sensitive to the environmental change affected by multiple anthropogenic disturbances [7,8]. For example, the yield of Chinese paddlefish (Psephurus gladius) in the Yangtze River basin rapidly declined from 25 t in 1970s, with the timing of extinction likely by 2005 and no later than by 2010, due to being increasingly affected by various anthropogenic stressors [48]. Chinese sturgeon (Acipenser sinensis) and reeves shad (Tenualosa reevesii) were extirpated in the Yangtze River basin after construction of the Gezhouba Dam and the Three Gorges Dam [8]. Dam construction caused the hydrological disconnection of river networks, fragmented aquatic habitats, and changed hydrological conditions, resulting in changes of fish diversity [49,50,51]. Some consequences of damming have been highlighted to cause a decline in freshwater species richness in the river networks [10,52,53]. River ecosystems have experienced hydrological disconnection affected by dam constructions, resulting in fish fauna homogenization [8,54,55,56]. This study demonstrated an overall homogenization trend in the fish fauna of the Lushan National Nature Reserve, with a 4% increase in taxonomic similarity among rivers over time. In addition, water pollution has caused the deterioration of water quality (e.g., increased N and P concentrations) and indirectly affected fish diversity [8]. Domestic sewage and tourism garbage pollution has changed the water quality of the reserve, resulting in a decrease in the sensitive fish species in this study. Therefore, freshwater fish in this study area may be under multiple disturbances, exposing the species to greater risks, so study on the interactions between multiple stressors needs further attention [57,58,59,60].
Our results demonstrated that the community structure of fish was significantly different among rivers, and environmental filtering was more important than competition in affecting the fish diversity. Similarly, other studies also reported that environmental filtering was the main contributor to the community construction of fish [60,61]. The predominant role of environmental filtering can be attributed to the strong environmental characteristics across rivers. These rivers in the Lushan National Nature Reserve cover a wide range of habitat divergence, which further augments the importance of environmental filtering. Environmental characteristics are important for determining community structure of fish [62,63,64,65]. Several recent studies have also highlighted the deterministic role of environmental variables (e.g., nutrients) in affecting fish assemblages [66,67,68]. For example, some temperature-sensitive species showed different changes in distribution, based on bottom temperature variability [66], while some other species’ dispersal was based on changes in the ambient water temperature, and their dispersal was related to length and maturity levels [67]. The frequent discharge of warm or cold water, flow regime changes, and other environmental variables in the downstream areas affected by dams can establish a novel environmental filter, selecting species with corresponding traits for living in downstream sites [68]. Therefore, it is not surprising that environmental filtering appears to be the major determinants of fish diversity in the study area.

5. Conclusions

As human activities continue to transform global freshwater ecosystems, a key objective is to develop dynamic strategies for the conservation and management of fish diversity to adapt to changing environmental conditions, while maintaining a natural biogeographic pattern [62,69,70]. Multi-faceted diversity (i.e., species, functional, and phylogenetic diversity) over space and time is considered key to pinpointing the drivers of community variability, which can help inform conservation prioritization and planning [20,21,22]. Our results demonstrated that the species diversity and phylogenetic diversity of fish in the Lushan National Nature Reserve experienced a decline from 2008 to 2021, and the community structure of fish was significantly different among rivers, while environmental filtering was the main contributor to the community construction of fish. These findings provide new insight into biodiversity conservation and the restoration of river ecosystems in the reserve. Given that the fish species diversity and phylogenetic diversity indices were greatly impacted by multiple anthropogenic disturbances, we suggest related biodiversity conservation should be carried out, due to the need for environmental conservation in the reserve. We should appropriately manage small dams, land use, and water pollution, otherwise continuing environmental change will result in drastic losses of biodiversity, and local or global extinction may occur [3,8,44,45].

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ani12121544/s1, Figure S1: Species accumulation curves for fish at each area in the Lushan National Nature Reserve.Shaded areas represent the 95% confidence intervals; Table S1: The number of total, native and alien species in the Lushan National Nature Reserve in 2008 and 2021. The threatened species (i.e., CR, EN, VU or NT) are those according to threatened status based on Chinese Red-list results. Native species: Native to China; Alien species: Nonnative to China; Table S2: Composition of fish catch in the Lushan National Nature Reserve in 2021.

Author Contributions

Data curation, D.L.; Formal analysis, D.L., X.W., X.L. and S.O.; Funding acquisition, X.W., X.L. and S.O.; Investigation, D.L., Z.W., C.J., Y.Z., Q.Z., F.Y., Y.F., M.Z., X.W. and S.O.; Methodology, D.L., X.L. and S.O.; Resources, Q.Z., F.Y., Y.F. and M.Z.; Software, D.L., Y.Z. and X.L.; Writing—original draft, D.L., X.W., X.L. and S.O.; Writing—review and editing, X.W., X.L. and S.O. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding or This research was funded by the investigation and monitoring of aquatic biological resources in key waters of Jiangxi province, and the Educational Commission of Guangdong Province of China (2021ZDZX4054). And The APC was funded by the investigation and monitoring of aquatic biological resources in key waters of Jiangxi province.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

All data included in this study are available upon request by contact with the corresponding author.

Acknowledgments

Funding for this research project came from the investigation and monitoring of aquatic biological resources in key waters of Jiangxi province, and the Educational Commission of Guangdong Province of China (2021ZDZX4054). The authors alone are responsible for the content and writing of this article.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Bruton, M.N. Have fishes had their chips? The dilemma of threatened fishes. Environ. Biol. Fish. 1995, 43, 1–27. [Google Scholar] [CrossRef]
  2. Duncan, J.R.; Lockwood, J.L. Extinction in a field of bullets: A search for causes in the decline of the world’s freshwater fishes. Biol. Conserv. 2001, 102, 97–105. [Google Scholar] [CrossRef]
  3. Arthington, A.H.; Dulvy, N.K.; Gladstone, W.; Winfield, I.J. Fish conservation in freshwater and marine realms: Status, threats and management. Aquat. Conserv. 2016, 26, 838–857. [Google Scholar] [CrossRef] [Green Version]
  4. Oberdorff, T.; Pont, D.; Hugueny, B.; Porcher, J. Development and validation of a fish-based index for the assessment of ‘river health’ in France. Freshw. Biol. 2002, 47, 1720–1734. [Google Scholar] [CrossRef]
  5. Li, T.; Huang, X.; Jiang, X.; Wang, X. Assessment of ecosystem health of the Yellow River with fish index of biotic integrity. Hydrobiologia 2018, 814, 31–43. [Google Scholar] [CrossRef]
  6. Flecker, A. Biodiversity Conservation in Running WatersIdentifying the major factors that threaten destruction of riverine species and ecosystems. BioScience 1993, 43, 32–43. [Google Scholar]
  7. Fu, C.; Wu, J.; Chen, J.; Wu, Q.; Lei, G. Freshwater fish biodiversity in the Yangtze River basin of China: Patterns, threats and conservation. Biodivers. Conserv. 2003, 12, 1649–1685. [Google Scholar] [CrossRef]
  8. Liu, X.; Qin, J.; Xu, Y.; Ouyang, S.; Wu, X. Biodiversity decline of fish assemblages after the impoundment of the Three Gorges Dam in the Yangtze River Basin, China. Rev. Fish Biol. Fish. 2019, 29, 177–195. [Google Scholar] [CrossRef]
  9. Bongaarts, J. Summary for policymakers of the global assessment report on biodiversity and ecosystem services of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services. Popul. Dev. Rev. 2019, 45, 680–681. [Google Scholar] [CrossRef] [Green Version]
  10. Agostinho, A.A.; Bonecker, C.C.; Gomes, L.C. Effects of water quantity on connectivity: The case of the upper Paraná River floodplain. Ecohydrol. Hydrobiol. 2009, 9, 99–113. [Google Scholar] [CrossRef]
  11. Lévêque, C.; Oberdorff, T.; Paugy, D.; Stiassny, M.; Tedesco, P. Global diversity of fish (Pisces) in freshwater. Hydrobiologia 2007, 595, 545–567. [Google Scholar] [CrossRef]
  12. Cheng, S.T.; Herricks, E.E.; Tsai, W.P.; Chang, F.J. Assessing the natural and anthropogenic influences on basin-wide fish species richness. Sci. Total Environ. 2016, 572, 825–836. [Google Scholar] [CrossRef] [PubMed]
  13. Jiang, X.; Zheng, P.; Cao, L.; Pan, B. Effects of long-term floodplain disconnection on multiple facets of lake fish biodiversity: Decline of alpha diversity leads to a regional differentiation through time. Sci. Total Environ. 2020, 763, 144177. [Google Scholar] [CrossRef] [PubMed]
  14. Wang, J.; Chen, L.; Tang, W.; Heino, J.; Jiang, X. Effects of dam construction and fish invasion on the species, functional and phylogenetic diversity of fish assemblages in the Yellow River Basin. J. Environ. Manag. 2021, 293, 112863. [Google Scholar] [CrossRef]
  15. Brosse, S.; Grenouillet, G.; Gevrey, M.; Khazraie, K.; Tudesque, L. Small-scale gold mining erodes fish assemblage structure in small neotropical streams. Biodivers. Conserv. 2011, 20, 1013–1026. [Google Scholar] [CrossRef]
  16. Schmera, D.; Heino, J.; Podani, J.; Ers, T.; Dolédec, S. Functional diversity: A review of methodology and current knowledge in freshwater macroinvertebrate research. Hydrobiologia 2017, 787, 27–44. [Google Scholar] [CrossRef] [Green Version]
  17. Webb, C.O.; Ackerly, D.; Mcpeek, M.A.; Donoghue, M.J. Phylogenies and community ecology. Annu. Rev. Ecol. Syst. 2002, 33, 475–505. [Google Scholar] [CrossRef] [Green Version]
  18. Graham, C.H.; Fine, P. Phylogenetic beta diversity: Linking ecological and evolutionary processes across space in time. Ecol. Lett. 2010, 11, 1265–1277. [Google Scholar] [CrossRef]
  19. Winter, M.; Schweiger, O.; Klotz, S.; Nentwig, W.; Andriopoulos, P.; Arianoutsou, M.; Basnou, C.; Delipetrou, P.; Didziulis, V.; Hejda, M. Plant extinctions and introductions lead to phylogenetic and taxonomic homogenization of the European flora. Proc. Natl. Acad Sci. USA 2009, 106, 21721–21725. [Google Scholar] [CrossRef] [Green Version]
  20. Strecker, A.L.; Olden, J.D.; Whittier, J.B.; Paukert, C.P. Defining conservation priorities for freshwater fishes according to taxonomic, functional, and phylogenetic diversity. Ecol. Appl. 2011, 21, 3002–3013. [Google Scholar] [CrossRef] [Green Version]
  21. Pool, T.K.; Grenouillet, G.; Villéger, S.; Ricciardi, A. Species contribute differently to the taxonomic, functional, and phylogenetic alpha and beta diversity of freshwater fish communities. Divers. Distrib. 2015, 20, 1235–1244. [Google Scholar] [CrossRef]
  22. Su, G.; Logez, M.; Xu, J.; Tao, S.; Brosse, S. Human impacts on global freshwater fish biodiversity. Science 2021, 371, 835–838. [Google Scholar] [CrossRef] [PubMed]
  23. Xun, G.; Quanan, W. The function and significance of reservation in the conserving biological diversity. Guihaia 1993, 4, 359–366. [Google Scholar]
  24. Liu, X.; Wang, L. Biodiversity Study and Research in Mount Lu Nature Reserve, Jiangxi Province; Science Press: Beijing, China, 2010. [Google Scholar]
  25. Jin, T.; Zhao, M.; Wu, J.; Li, J.; Li, H. Distribution characteristics and influencing factors of glomalin-related soil protein in the rhizosphere of common tree species in evergreen broad-leaved forest of Lushan. Chin. J. Ecol. 2021, 40, 11. [Google Scholar]
  26. Zhang, L.; Li, B.; Yang, W. Forest vegetation classification and its variation in Lushan Nature Reserve using Proba-V vegetation products. Prog. Geogr. 2021, 40, 10. [Google Scholar] [CrossRef]
  27. Huang, L.; Wu, Z.; Hu, M.; Li, Q.; Zong, D.S.; Wan, Z.Q.; Zhao, W.Q. Species Diversity of Fish of Lushan Nature Reserve in Jiangxi Province. J. Nanchang Univ. Nat. Sci. 2008, 32, 161–164. [Google Scholar]
  28. Chen, Y.Y. Fauna Sinica, Osteichthyes; Science Press: Beijing, China, 1998. [Google Scholar]
  29. Chu, X.; Zheng, B.; Dai, D. Fauna Sinica: Osteichthyes Siluriformes; Science Press: Beijing, China, 1999. [Google Scholar]
  30. Yue, P. Fauna Sinica: Osteichthyes Cypriniformes III; Science Press: Beijing, China, 2000. [Google Scholar]
  31. Froese, R.; Pauly, D.; FishBase. World Wide Web Electronic Publication. 2014. Available online: http://www.fishbase.org (accessed on 5 May 2022).
  32. Chao, A.; Ma, K.H.; Hsieh, T.C. iNEXT (iNterpolation and EXTrapolation) Online: Software for Interpolation and Extrapolation of Species Diversity. Program and User’s Guide. 2016. Available online: http://chao.stat.nthu.edu.tw/wordpress/software_download/ (accessed on 1 March 2022).
  33. Pianka, E.R. Ecology of the Agamid Lizard Amphibolurus isolepis in Western Australia. Copeia 1971, 3, 527–536. [Google Scholar] [CrossRef]
  34. Magurran, A.E. Ecological Diversity and Its Measurement; Princeton University Press: London, UK, 1988. [Google Scholar]
  35. Peet, R.K. The Measurement of Species Diversity. Annu. Rev. Ecol. Syst. 1974, 5, 285–307. [Google Scholar] [CrossRef]
  36. Baselga, A. Partitioning the turnover and nestedness components of beta diversity. Glob. Ecol. Biogeogr. 2010, 19, 134–143. [Google Scholar] [CrossRef]
  37. Carvalho, J.C.; Cardoso, P.; Gomes, P. Determining the relative roles of species replacement and species richness differences in generating beta-diversity patterns. Glob. Ecol. Biogeogr. 2012, 21, 760–771. [Google Scholar] [CrossRef]
  38. R Core Team. R: A Language and Environment for Statistical Computing; R Foundation for Statistical Computing: Vienna, Austria, 2009. [Google Scholar]
  39. Baselga, A.; Orme, C. betapart: An R package for the study of beta diversity. Methods Ecol. Evol. 2012, 3, 808–812. [Google Scholar] [CrossRef]
  40. Oksanen, J.; Blanchet, F.G.; Kindt, R.; Legendre, P. Vegan: Community Ecology Package. R Package Version 2.3-2. 2011. Available online: https://cran.r-project.org (accessed on 15 February 2022).
  41. Clarke, K.R.; Gorley, R.N. PRIMER v6: User Manual/Tutorial; PRIMER-E: Plymouth, UK, 2006. [Google Scholar]
  42. Clarke, K.R. Non-parametric multivariate analyses of changes in community structure. Austral. Ecol. 2010, 18, 117–143. [Google Scholar] [CrossRef]
  43. Webb, C.O. picante: R tools for integrating phylogenies and ecology. Bioinformatics 2010, 26, 1463–1464. [Google Scholar]
  44. Barbarossa, V.; Schmitt, R.; Huijbregts, M.; Zarfl, C.; Schipper, A.M. Impacts of current and future large dams on the geographic range connectivity of freshwater fish worldwide. Proc. Natl. Acad. Sci. USA 2020, 117, 3648–3655. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Díaz, G.; Górski, K.; Heino, J.; Arriagada, P. The Longest Fragment Drives Fish Beta Diversity in Fragmented River Networks: Implications for River Management And Conservation. Sci. Total Environ. 2021, 766, 144323. [Google Scholar] [CrossRef] [PubMed]
  46. Kang, B.; He, D.; Perrett, L.; Wang, H.; Hu, W.; Deng, W.; Wu, Y. Fish and fisheries in the Upper Mekong: Current assessment of the fish community, threats and conservation. Rev. Fish Biol. Fish. 2009, 19, 465. [Google Scholar] [CrossRef]
  47. Liu, X.; Qin, J.; Xu, Y.; Zhou, M.; Wu, X.; Ouyang, S. Biodiversity pattern of fish assemblages in Poyang Lake Basin: Threat and conservation. Ecol. Evol. 2019, 9, 11672–11683. [Google Scholar] [CrossRef]
  48. Zhang, H.; Jarić, I.; Roberts, D.L.; He, Y.; Du, H.; Wu, J.; Wang, C.; Wei, Q. Extinction of one of the world’s largest freshwater fishes: Lessons for conserving the endangered Yangtze fauna. Sci. Total Environ. 2020, 710, 136242. [Google Scholar] [CrossRef]
  49. Bunn, S.E.; Arthington, A.H. Basic Principles and Ecological Consequences of Altered Flow Regimes for Aquatic Biodiversity. Environ. Manag. 2002, 30, 492–507. [Google Scholar] [CrossRef] [Green Version]
  50. Reidy, L.C.; Christer, N.; James, R.; Ng, R.Y. Implications of dam obstruction for global freshwater fish diversity. BioScience 2012, 62, 539–548. [Google Scholar]
  51. Lansac-Tôha, F.M.; Heino, J.; Quirino, B.A.; Moresco, G.A.; Peláez, O.; Meira, B.R.; Rodrigues, L.C.; Jati, S.; Lansac-Tôha, F.A.; Velho, L.F.M. Differently dispersing organism groups show contrasting beta diversity patterns in a dammed subtropical river basin. Sci. Total Environ. 2019, 691, 1271–1281. [Google Scholar] [CrossRef] [PubMed]
  52. Bonecker, C.C.; Simões, N.; Minte-Vera, C.V.; Lansac-Tôha, F.A.; Velho, L.F.M.; Agostinho, A. Temporal changes in zooplankton species diversity in response to environmental changes in an alluvial valley. Limnol. Ecol. Manag. Inl. Waters 2013, 43, 114–121. [Google Scholar] [CrossRef]
  53. Winemiller, K.O.; Mcintyre, P.B.; Castello, L.; Fluet-Chouinard, E.; Saenz, L. Balancing Hydropower and Biodiversity in the Amazon, Congo, and Mekong. Science 2016, 351, 128–129. [Google Scholar] [CrossRef] [Green Version]
  54. Vitule, J.R.S.; Skora, F. Homogenization of freshwater fish faunas after the elimination of a natural barrier by a dam in Neotropics. Divers. Distrib. 2012, 18, 111–120. [Google Scholar] [CrossRef]
  55. Petesse, M.L.; Petrere, M. Tendency towards homogenization in fish assemblages in the cascade reservoir system of the Tietê river basin, Brazil. Ecol. Eng. 2012, 48, 109–116. [Google Scholar] [CrossRef]
  56. Daga, V.S.; Skóra, F.; Padial, A.A.; Abilhoa, V.; Gubiani, É.A.; Vitule, J. Homogenization dynamics of the fish assemblages in Neotropical reservoirs: Comparing the roles of introduced species and their vectors. Hydrobiologia 2015, 746, 327–347. [Google Scholar] [CrossRef]
  57. Couto, T.B.; Olden, J.D. Global proliferation of small hydropower plants—Science and policy. Front. Ecol. Environ. 2018, 6, 91–100. [Google Scholar] [CrossRef]
  58. Grill, G.; Lehner, B.; Thiem, M.; Geenen, B. Mapping the world’s free-flowing rivers. Nature 2019, 569, 215–221. [Google Scholar] [CrossRef]
  59. Reid, A.J.; Carlson, A.K.; Creed, I.F.; Eliason, E.J.; Cooke, S.J. Emerging threats and persistent conservation challenges for freshwater biodiversity. Biol. Rev. 2018, 94, 849–873. [Google Scholar] [CrossRef] [Green Version]
  60. Tickner, D.; Opperman, J.J.; Abell, R.; Acreman, M.; Young, L. Bending the Curve of Global Freshwater Biodiversity Loss—An Emergency Recovery Plan. BioScience 2020, 70, 330–342. [Google Scholar] [CrossRef]
  61. Gthe, E.; Pedersen, A.B.; Larsen, P.W.; Graeber, D.; Kristensen, E.A.; Friberg, N. Environmental and spatial controls of taxonomic versus trait composition of stream biota. Freshw. Biol. 2017, 62, 397–413. [Google Scholar] [CrossRef]
  62. Heino, J.; Tolonen, K.T. Ecological drivers of multiple facets of beta diversity in a lentic macroinvertebrate metacommunity. Limnol. Oceanogr. 2017, 62, 2431–2444. [Google Scholar] [CrossRef]
  63. Mouchet, M.A.; Burns, M.; Garcia, A.M.; Vieira, J.P.; Mouillot, D. Invariant scaling relationship between functional dissimilarity and co-occurrence in fish assemblages of the Patos Lagoon estuary (Brazil): Environmental filtering consistently overshadows competitive exclusion. Oikos 2013, 122, 247–257. [Google Scholar] [CrossRef]
  64. Sá-Oliveira, J.C.; Hawes, J.E.; Isaac-Nahum, V.J.; Peres, C.A. Upstream and downstream responses of fish assemblages to an eastern Amazonian hydroelectric dam. Freshw. Biol. 2015, 60, 2037–2050. [Google Scholar] [CrossRef]
  65. Guo, Q.; Liu, X.; Ao, X.; Qin, J.; Wu, X.; Ouyang, S. Fish diversity in the middle and lower reaches of the Ganjiang River of China: Threats and conservation. PLoS ONE 2018, 13, e205116. [Google Scholar] [CrossRef] [Green Version]
  66. Methratta, E.T.; Link, J.S. Ontogenetic variation in habitat association for four groundfish species in the Gulf of Maine—Georges Bank region. Mar. Ecol. Prog. Ser. 2007, 338, 169–181. [Google Scholar] [CrossRef]
  67. Jones, J.B.; Campana, S.E. Stable Oxygen Isotope Reconstruction of Ambient Temperature during the Collapse of a Cod (Gadus Morhua) Fishery. Ecol. Appl. 2009, 19, 1500–1514. [Google Scholar] [CrossRef] [Green Version]
  68. Bruno, D.; Belmar, O.; Maire, A.; Morel, A.; Dumont, B.; Datry, T. Structural and functional responses of invertebrate communities to climate change and flow regulation in alpine catchments. Glob. Chang. Biol. 2019, 25, 1612–1628. [Google Scholar] [CrossRef] [Green Version]
  69. Olden, J.D.; Kennard, M.K.; Leprieur, F.; Tedesco, P.A.; Winemiller, K.O.; García-Berthou, E. Conservation biogeography of freshwater fishes: Past progress and future directions. Divers. Distrib. 2010, 16, 496–513. [Google Scholar] [CrossRef]
  70. Soininen, J.; Heino, J.; Wang, J. A meta-analysis of nestedness and turnover components of beta diversity across organisms and ecosystems. Glob. Ecol. Biogeogr. 2018, 27, 96–109. [Google Scholar] [CrossRef] [Green Version]
Animals 12 01544 g001 550
Figure 1. Map showing the study area of the Lushan National Nature Reserve. Black stars represent the sampling sites.
Figure 1. Map showing the study area of the Lushan National Nature Reserve. Black stars represent the sampling sites.
Animals 12 01544 g001
Animals 12 01544 g002 550
Figure 2. Temporal and spatial change in the diversity of fish in the Lushan National Nature Reserve in 2008 (A) and 2021 (B). River codes are the same as in Table 1. Blue, orange, red, and purple circles represent the H, J, D, and F, respectively. H: Shannon–Weiner index; D: Margalef diversity index; F: Simpson dominance index; J: Pielou evenness index.
Figure 2. Temporal and spatial change in the diversity of fish in the Lushan National Nature Reserve in 2008 (A) and 2021 (B). River codes are the same as in Table 1. Blue, orange, red, and purple circles represent the H, J, D, and F, respectively. H: Shannon–Weiner index; D: Margalef diversity index; F: Simpson dominance index; J: Pielou evenness index.
Animals 12 01544 g002
Animals 12 01544 g003 550
Figure 3. Fish species compositional dissimilarity for 2008 and 2021, quantified by the Sørensen dissimilarity index (βsor), its spatial turnover component (βsim), and its nestedness component (βsne), in the Lushan National Nature Reserve.
Figure 3. Fish species compositional dissimilarity for 2008 and 2021, quantified by the Sørensen dissimilarity index (βsor), its spatial turnover component (βsim), and its nestedness component (βsne), in the Lushan National Nature Reserve.
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Animals 12 01544 g004 550
Figure 4. The Bray–Curtis resemblance matrix and the non-metric multidimensional scaling (NMDS) ordination for the community structure of fish in 2008 (A,B) and 2021 (C,D) in Lushan National Nature Reserve. River codes are the same as in Table 1.
Figure 4. The Bray–Curtis resemblance matrix and the non-metric multidimensional scaling (NMDS) ordination for the community structure of fish in 2008 (A,B) and 2021 (C,D) in Lushan National Nature Reserve. River codes are the same as in Table 1.
Animals 12 01544 g004
Table
Table 1. The phylogenetic distance (MPD) randomization and observed, standard deviation of random values (SD) and net relatedness index (NRI) of fish in the Lushan National Nature Reserve in 2008 and 2021.
Table 1. The phylogenetic distance (MPD) randomization and observed, standard deviation of random values (SD) and net relatedness index (NRI) of fish in the Lushan National Nature Reserve in 2008 and 2021.
RiverCodes20082021
MPD RandomizationMPD ObservedSDNRIMPD RandomizationMPD ObservedSDNRI
TaohuayuanTHY0.0610.0600.0080.1730.1220.1090.0190.658
GuizongGZ0.1590.1490.0170.5870.1020.1010.0140.076
XiufengXF0.2100.1890.0210.9800.2000.1960.0200.230
GuanyinqiaoGYQ0.1720.1550.0200.8190.2200.2230.021−0.145
GaolongGL0.2010.1760.0181.3340.1370.1230.0170.812
TongyuanTY0.0800.0630.0101.6940.0100.0110.002−0.554
JiandaoxiaJDX0.0980.0960.0120.1800.0910.0980.013−0.536
LianhuaLH0.1150.1140.0140.1150.0510.0560.008−0.676
WeijiaWJNANANANA0.2150.2100.0220.211
ShimenjianSMJ0.0830.0760.0110.6150.1010.1060.013−0.426
WuliqiaoWLQ0.2220.2070.0190.8370.1160.1120.0130.285
HaihuiHH0.1780.1640.0220.6240.1460.1190.0191.372
ShanshangOTM0.1250.1380.017−0.823————
LongmengouLMG————0.0290.0310.006−0.337
Total0.1880.1710.0171.0020.1310.1240.0140.510
Table
Table 2. Dissimilarity analysis (SIMPER) between fish assemblages in the Lushan National Nature Reserve in 2008 and 2021.
Table 2. Dissimilarity analysis (SIMPER) between fish assemblages in the Lushan National Nature Reserve in 2008 and 2021.
2008
SpeciesA group–B group
(average dissimilarity = 100)		A group–C group
(average dissimilarity = 100)		B group–C group
(average dissimilarity = 78.12)
Average dissimilarityContribution rate
(%)		Average dissimilarityContribution rate
(%)		Average dissimilarityContribution rate
(%)
Zacco platypus46.5146.5145.1645.1633.4542.82
Rhynchocypris oxycephala11.4111.41——9.0811.63
Acrossocheilus parallens4.744.7414.1614.165.116.54
Rhinogobius giurinus15.5615.56——8.5010.89
Rhodeus ocellatus——14.5114.515.577.13
Misgurnus anguillicaudatus8.318.313.773.774.685.99
Hemibarbus maculatus3.403.407.147.143.594.59
Opsariichthys bidens3.263.26——2.953.78
Liobagrus anguillicauda——5.465.46——
2021
SpeciesA group–B group
(average dissimilarity = 87.2)		A group–C group
(average dissimilarity = 86.74)		B group–C group
(average dissimilarity = 49.94)
Average dissimilarityContribution rate
(%)		Average dissimilarityContribution rate
(%)		Average dissimilarityContribution rate
(%)
Zacco platypus34.5739.6447.4354.6826.7353.53
Opsariichthys bidens14.6316.7711.7013.492.354.71
Acrossocheilus parallens10.4511.99——7.9916.01
Rhodeus ocellatus7.949.108.419.70——
Rhinogobius giurinus7.208.264.765.492.785.57
Misgurnus anguillicaudatus5.336.116.797.831.843.69
Liobagrus anguillicauda————1.482.96
Monopterus albus————1.232.47
Odontobutis sinensis————1.192.39
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Luo, D.; Wan, Z.; Jia, C.; Zhu, Y.; Zou, Q.; Ye, F.; Feng, Y.; Zhang, M.; Wu, X.; Liu, X.; et al. Temporal Changes of Fish Diversity and Driver Factors in a National Nature Reserve, China. Animals 2022, 12, 1544. https://doi.org/10.3390/ani12121544

AMA Style

Luo D, Wan Z, Jia C, Zhu Y, Zou Q, Ye F, Feng Y, Zhang M, Wu X, Liu X, et al. Temporal Changes of Fish Diversity and Driver Factors in a National Nature Reserve, China. Animals. 2022; 12(12):1544. https://doi.org/10.3390/ani12121544

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Luo, Dongmei, Ziwei Wan, Chenchen Jia, Yiqiu Zhu, Qin Zou, Fangfei Ye, Yan Feng, Mi Zhang, Xiaoping Wu, Xiongjun Liu, and et al. 2022. "Temporal Changes of Fish Diversity and Driver Factors in a National Nature Reserve, China" Animals 12, no. 12: 1544. https://doi.org/10.3390/ani12121544

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