Toxocara canis Infection Alters mRNA Expression Profiles of Peripheral Blood Mononuclear Cells in Beagle Dogs at the Lung Infection Period
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Approval
2.2. Animal and Sample Collection
2.3. RNA Extraction and RNA Sequencing Analysis
2.4. GO Annotation and KEGG Pathway Enrichment Analysis
2.5. Quantitative Real Time RT-PCR (qRT-PCR)
3. Results
3.1. Confirmation of T. canis Infection and Overview of RNA Sequencing
3.2. GO Annotation and KEGG Pathway Enrichment Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Raw Reads | Clean Reads | Clean Bases | Q20 | Q30 | GC Content |
---|---|---|---|---|---|---|
AS96hC1 | 46,080,628 | 44,799,936 | 6.72G | 97.29% | 92.89% | 49.98% |
AS96hC2 | 46,016,190 | 44,600,408 | 6.69G | 97.58% | 93.58% | 52.14% |
AS96hC3 | 43,874,812 | 42,545,422 | 6.38G | 97.51% | 93.32% | 48.84% |
AS96hT1 | 44,745,214 | 43,474,512 | 6.52G | 97.47% | 93.31% | 48.98% |
AS96hT2 | 45,812,738 | 44,663,176 | 6.70G | 97.83% | 94.07% | 52.66% |
AS96hT3 | 46,227,832 | 44,959,256 | 6.74G | 97.49% | 93.26% | 49.18% |
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Cai, L.; Zou, Y.; Xu, Y.; Li, H.-Y.; Xie, S.-C.; Zhu, X.-Q.; Zheng, W.-B. Toxocara canis Infection Alters mRNA Expression Profiles of Peripheral Blood Mononuclear Cells in Beagle Dogs at the Lung Infection Period. Animals 2022, 12, 1517. https://doi.org/10.3390/ani12121517
Cai L, Zou Y, Xu Y, Li H-Y, Xie S-C, Zhu X-Q, Zheng W-B. Toxocara canis Infection Alters mRNA Expression Profiles of Peripheral Blood Mononuclear Cells in Beagle Dogs at the Lung Infection Period. Animals. 2022; 12(12):1517. https://doi.org/10.3390/ani12121517
Chicago/Turabian StyleCai, Lang, Yang Zou, Yue Xu, Hao-Yu Li, Shi-Chen Xie, Xing-Quan Zhu, and Wen-Bin Zheng. 2022. "Toxocara canis Infection Alters mRNA Expression Profiles of Peripheral Blood Mononuclear Cells in Beagle Dogs at the Lung Infection Period" Animals 12, no. 12: 1517. https://doi.org/10.3390/ani12121517